Inespecificidad persistente en amplificación isotérmica mediada por loop para diagnóstico de malaria

Persistent non-specificity in loop-mediated insothermal amplication for malaria diagnosis

Objetivo. Describir el ensayo realizado para la estandarización de la técnica de diagnóstico para malaria, denominada amplificación isotérmica mediada por loop (LAMP). Métodos. Se extrajeron 48 muestras divididas en 22 muestras para P. falciparum, 20 de P. vivax y 6 negativas para las mismas especies. Resultados. Se observaron falsos positivos, amplificaciones específicas que evidencian la inespecificidad de la técnica. Se propone estudiar los amplicones obtenidos por medio de restricción de fragmentos y secuenciación para elucidar el origen del problema.

1. World Health Organization. World Malaria Report 2015. Geneva, Switzerland; 2015. 280 p.
2. World Health Organization. Guidelines For The Treatment of Malaria. 2015.
3. SIVIGILA. Vigilancia rutinaria por eventos departamental a semana 52 [Internet]. Bogotá; 2015. Available from: http://www. ins.gov.co/lineas-de-accion/Subdireccion-Vigilancia/sivigila/ Paginas/vigilancia-rutinaria.aspx (Agosto de 2015).
4. SIVIGILA. Vigilancia Rutinaria por evento Departamental a semana 23, 2016 [Internet]. Bogotá; 2016. Available from: http://www.ins.gov.co/lineas-de-accion/Subdireccion-Vigilancia/ sivigila/Paginas/vigilancia-rutinaria.aspx (Junio de 2016)
5. Programa Mundial de Paludismo. Estratégica técnica mundial para o paludismo 2016–2030. Genebra,Suíça; 2016.
6. Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, et al. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 2000; 28(12): E63.
7. Mori Y, Nagamine K, Tomita N, Notomi T. Detection of loopmediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem Biophys Res Commun. 2001;289(1): 150–4.
8. Ferrara M, Perrone G, Gallo A, Epifani F, Visconti A., Susca
9. A. Development of loop-mediated isothermal amplification (LAMP) assay for the rapid detection of Penicillium nordicum in dry-cured meat products. Int J Food Microbiol [Internet]. Elsevier B.V.; 2015;202:42–7. Available from: http://linkinghub.elsevier. com/retrieve/pii/S0168160515001014
10. Diribe O, North S, Sawyer J, Roberts L, Fitzpatrick N, La Ragione R. Design and application of a loop-mediated isothermal amplification assay for the rapid detection of Staphylococcus pseudintermedius. J Vet Diagn Invest [Internet]. 2014;26(1):42–8. Available from: http://www.ncbi.nlm.nih.gov/ pubmed/24398904
11. Hsieh K, Mage PL, Csordas AT, Eisenstein M, Soh HT. Simultaneous elimination of carryover contamination and detection of DNA with uracil-DNA-glycosylase-supplemented loop-mediated isothermal amplification (UDG-LAMP). Chem Commun (Camb) [Internet]. 2014;50(28):3747–9. Available from: http://www.ncbi.nlm.nih.gov/pubmed/24577617
12. Kong Q-M, Lu S-H, Tong Q-B, Lou D, Chen R, Zheng B, et al. Loop-mediated isothermal amplification (LAMP): Early detection of Toxoplasma gondii infection in mice. Parasit Vectors [Internet]. BioMed Central Ltd; 2012;5(1):2. Available from: http://www.parasitesandvectors.com/content/5/1/2
13. Senarath KD, Usgodaarachchi RB, Navaratne V, Nagahawatte a., Wijayarathna CD, Alvitigala J, et al. Non Specific Amplification with the LAMP Technique in the Diagnosis of Tuberculosis in Sri Lankan Settings. J Tuberc Res [Internet]. 2014;02(04):168–72.
14. Available from: http://file.scirp.org/Html/3-1130069_51522. htm
15. Zhang X, Lowe SB, Gooding JJ. Brief review of monitoring methods for loop-mediated isothermal amplification (LAMP). Biosens Bioelectron [Internet]. Elsevier; 2014;61C:491–9. Available from: http://www.ncbi.nlm.nih.gov/pubmed/24949822
16. Yongkiettrakul S, Jaroenram W, Arunrut N, Chareanchim W, Pannengpetch S, Suebsing R, et al. Application of loop-mediated isothermal amplification assay combined with lateral flow dipstick for detection of Plasmodium falciparum and Plasmodium vivax. Parasitol Int [Internet]. Elsevier Ireland Ltd; 2014;63(6):777–84. Available from: http://dx.doi.org/10.1016/j.parint.2014.06.004
17. Lee P-W, Ji D-D, Liu C-T, Rampao HS, do Rosario VE, Lin I-F, et al. Application of loop-mediated isothermal amplification for malaria diagnosis during a follow-up study in São Tomé. Malar J [Internet]. 2012;11(1):408. Available from: http://www.pubmedcentral.nih.gov/articlerender. fcgi?artid=3528453&tool=pmcentrez&rendertype=abstract
18. Surabattula R, Vejandla MP, Mallepaddi PC, Faulstich K, Polavarapu R. Simple, rapid, inexpensive platform for the diagnosis of malaria by loop mediated isothermal amplification (LAMP). Exp Parasitol [Internet]. Elsevier Inc.; 2013;134(3):333–40.Availablefrom: http://dx.doi.org/10.1016/j.exppara.2013.03.031
19. Karthik K, Rathore R, Thomas P, Arun TR, Viswas KN, Dhama K, et al. New closed tube loop mediated isothermal amplification assay for prevention of product cross-contamination. MethodsX [Internet]. Elsevier B.V.; 2014;1:e137–43. Available from: http:// dx.doi.org/10.1016/j.mex.2014.08.009
20. Lucchi NW, Ljolje D, Silva-Flannery L, Udhayakumar V. Use of Malachite Green-Loop Mediated Isothermal Amplification for Detection of Plasmodium spp. Parasites. PLoS One [Internet]. 2016;11(3):e0151437. Available from: http://journals.plos.org/ plosone/article?id=10.1371/journal.pone.0151437
21. Goto M, Honda E, Ogura A, Nomoto A, Hanaki KI. Colorimetric detection of loop-mediated isothermal amplification reaction by using hydroxy naphthol blue. Biotechniques. 2009;46(3):167–72.
22. Britton S, Cheng Q, Sutherland CJ, McCarthy JS. A simple, high-throughput, colourimetric, field applicable loopmediated isothermal amplification (HtLAMP) assay for malaria elimination. Malar J [Internet]. BioMed Central; 2015;14:335.
23. Available from: http://www.pubmedcentral.nih.gov/articlerender. fcgi?artid=4552465&tool=pmcentrez&rendertype=abstract
24. Lau Y-L, Fong M-Y, Mahmud R, Chang P-Y, Palaeya V, Cheong F-W, et al. Specific, sensitive and rapid detection of human Plasmodium knowlesi infection by loop-mediated isothermal amplification (LAMP) in blood samples. Malar J [Internet]. 2011;10:197.
25. Available from: http://www.pubmedcentral.nih.gov/articlerender. fcgi?artid=3156799&tool=pmcentrez&rendertype=abstract
26. Lu F, Gao Q, Zhou H, Cao J, Wang W, Lim CS, et al. Molecular test for vivax malaria with loop-mediated isothermal amplification method in central China. Parasitol Res. 2012;110(6):2439–44.
27. Snounou G, Viriyakosol S, Xin Ping Zhu, Jarra W, Pinheiro L, do Rosario VE, et al. High sensitivity of detection of human malaria parasites by the use of nested polymerase chain reaction. Mol Biochem Parasitol. 1993;61(2):315–20.
28. Han ET, Watanabe R, Sattabongkot J, Khuntirat B, Sirichaisinthop J, Iriko H, et al. Detection of four Plasmodium species by genus- and species-specific loop-mediated isothermal amplification for clinical diagnosis. J Clin Microbiol. 2007;45(8):2521–8.
29. Tanner N a., Evans TC. Loop-mediated isothermal amplification for detection of nucleic acids. Curr Protoc Mol Biol. 2014;2014(January):15.14.1–15.14.14.
30. Dieffenbach CW, Dveksler GS. Setting up a PCR laboratory. Genome Res. 1993;3(2):5–14.
31. Pelt V. Ensuring PCR Quality – Laboratory Organization, PCR Optimization and Controls. In: Principles PCR. 2008.
32. Yongkiettrakul S, Jaroenram W, Arunrut N, Chareanchim W, Pannengpetch S, Suebsing R, et al. Application of loop-mediated isothermal amplification assay combined with lateral flow dipstick for detection of Plasmodium falciparum and Plasmodium vivax. Parasitol Int [Internet]. Elsevier Ireland Ltd; 2014;63(6):777–84. Available from: http://dx.doi.org/10.1016/j.parint.2014.06.004
33. Millan J, Yunda L. An Open-Access Web-based medical image atlas for collaborative Medical image sharing, processing, Web Semantic searching and analysis with uses in medical training, research and second opinion of cases. Nova. 2014;12(22):143-50
34. ==========================================
35. DOI: http://dx.doi.org/10.22490/24629448.1748