Persistent non-specificity in loop-mediated insothermal amplication for malaria diagnosis

Inespecificidad persistente en amplificación isotérmica mediada por loop para diagnóstico de malaria

Published 2016-12-31

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Vol. 14 No. 26 (2016)
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Artículo Original Producto de Investigación
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Pachón Meza, L. C., & Barrera Ayala, S. M. (2016). Persistent non-specificity in loop-mediated insothermal amplication for malaria diagnosis. NOVA, 14(26), 29-34. https://doi.org/10.22490/24629448.1748
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https://doi.org/10.22490/24629448.1748
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Laura Cristina Pachón Meza
Instituto Nacional de Salud
    Sandra Milena Barrera Ayala
    Instituto Nacional de Salud
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      Objetive. The present study describes the assay performed to standardize a malaria diagnostic DNA test, called loop-mediated isothermal amplification (LAMP). Methods. Extractions from 48 Plasmodium -infected blood samples (22P. falciparum-positive samples, 20 P. vivax-positive samplesand 6 negative control samples). Results. The data show false positives, unspecific amplifications that demonstrate the low specificity of the test. We propose to study the amplicons obtained by restriction fragment and sequencing to investigate the underlying cause of the problem.

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      35. DOI: http://dx.doi.org/10.22490/24629448.1748
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