Culture xerophile microorganisms from the Tatacoa semiarid zone (Colombia).

Microorganismos xerófilos cultivables de la zona semiárida de la Tatacoa (Colombia).

Introduction. Xerophilic microorganisms have become more relevant for conducting research related to their adaptive mechanisms against water stress, as well as the characterization and identification of their habitats. In Colombia, semi-arid and desert areas such as the Tatacoa desert have been little studied at the microbial level. Objective. Isolation and characterization of cultivable xerophilic microorganisms from the soil of the semi-arid zone of Tatacoa, in the department of Huila (Colombia). Material and methods. For this, samples were collected in the sectors locally called Cuzco and La Victoria, which were processed for the isolation of xerophilic microorganisms in selective M40Y medium for subsequent macro and microscopic characterization, as well as evaluation by biochemical tests for the use of substrates. Results. 29 morphotypes were isolated among which it was possible to differentiate: Gram-positive bacilli and cocci present exclusively in the Cuzco sector and branched filamentous bacteria (Actinobacteria) only in the La Victoria sector. The presence of the genera Streptomyces, Micrococcus and Corynebacterium was established. Conclusions. The presence of microorganisms related to the mentioned genera will allow us to understand the possible interactions that occur in this ecosystem, which will contribute to the development of this place as a possible analogue of studies for the search for life on other planets such as Mars. In addition, promote more detailed studies where microorganisms that are useful for different biotechnological processes can be recovered.

1. Gargaud M. Encyclopedia of astrobiology, Berlin: Springer Science & Business Media; 2011. https://www.springer.com/gp/book/9783642112744
2. Scott W. Water Relations of Food Spoilage Microorganisms. Advances in Food Research. 1957;(7): 83-127. https://doi.org/10.1016/S0065-2628(08)60247-5
3. Pitt J, Hocking A. Fungi and food Spoilage. Verlag: Springer. 2009. https://www.springer.com/gp/book/9780387922065
4. Petrovič U, Gunde-Cimerman N, Zalar P. Xerotolerant mycobiota from high altitude anapurna soil, nepal. FEMS microbiology letters. 2000; 128(2): 339- 342. https://doi.org/10.1111/j.1574-6968.2000.tb08918.x
5. Sharma A,Sharma R, Devi T. Life at extreme conditions: Extremophiles and their biocatalytic potential. En: 3rd International conference on recent advances in engineering science and management. Pune: 2017. https://www.semanticscholar.org/paper/Life-at-extreme-conditions%3A-Extremophiles-and-their-Sharma-Sharma/4ebc1e2814fe20fe66396856bdd8d130704b5762
6. Srivastava A, Rai A, Kumar S, Kashyap P, Arora D. Extremophiles: Potential Sources of Biomolecules. En: Prakash S, Sharma R, Kunwar R. Recent advances in microbiology. Nueva York: NOVA; 2013. p.551-564. https://www.researchgate.net/publication/249314487_Extremophiles_Potential_Sources_of_Biomolecules
7. Dion P. Soil Biology and Agriculture in the Tropics. Berlin: Springer; 2010. https://www.springer.com/gp/book/9783642050756
8. Schulze-Makuch D, Houtkooper J. A perchlorate strategy for extreme xerophilic life on Mars. En: EPSC Abstracts. Rome; 2010. https://meetings.copernicus.org/epsc2010/abstracts/EPSC2010-308.pdf
9. Stevenson A, Burkhardt J, Cockell C, Cray J, Dijksterhuis J, Fox-Powell M, et al. Multiplication of microbes below 0.690 eater activity: Implications for terrestrial and extraterrestrial life. Environmental microbiology. 2015; 17(2): 257-277. https://doi.org/10.1111/1462-2920.12598
10. Lebre P, De Maayer P,Cowan D. Xerotolerant bacteria: surviving through a dry spell. Nature Reviews Microbiology. 2017; 15(5): 285-296. https://doi.org/10.1038/nrmicro.2017.16
11. Okoro C, Brown R, Jones A, Andrews B, Asenjo J, Goodfellow M, et al. Diversity of culturable actinomycetes in hyper-arid soils of the Atacama Desert, Chile,Antonie Van Leeuwenhoek. 2009; 95(2): 121-133. https://pubmed.ncbi.nlm.nih.gov/19052913/
12. Santhanam R, Okoro C, Rong C, Huang Y, Bull A, Weon H, et al. Streptomyces atacamensis sp. nov., isolated from and extreme hyper-arid soil of the Atacama Desert, Chile. International journal of systematic and evolutionary microbiology. 2012; 62(11): 2680-2684. https://doi.org/10.1099/ijs.0.038463-0
13. Santhanam R, Rong X, Huang Y, Andrews B, Asenjo J, Goodfellow M. Streptomyces bullii sp. nov., isolated from a hyper-arid Atacama Desert soil. Antonie Van Leeuwenhoek. 2013; 103(2): 367- 373. https://doi.org/10.1007/s10482-012-9816-x
14. Azua-Bustos A, Urrejola C, Vicuña R. Life at the dry edge: microorganisms of the Atacama Desert. FEBS Letters. 2012; 586(18): 2939-2945. https://doi.org/10.1016/j.febslet.2012.07.025
15. Piubeli F, De Lourdes M, Kishi L, Henrique-Silva F, García M, Mellado E. Phylogenetic profiling and diversity of bacterial communities in the death valley, an extreme habitat in the atacama desert. Indian journal of microbiology. 2015; 55(4): 392- 399. https://doi.org/10.1007/s12088-015-0539-3
16. Drees K, Neilson J, Betancourt J, Quade J, Henderson D, Pryor B, et al. Bacterial community structure in the hyperarid core of the Atacama Desert, Chile,Applied and Environmental Microbiology. 2006; 72(12): 7902-7908. https://aem.asm.org/content/72/12/7902
17. Direito S, Ehrenfreund P, Marees A, Staats M, Foing B, Roling W. A wide variety of putative extremophiles and large beta-diversity at the mars desert research station (utah). International Journal of Astrobiology. 2011; 10(3): 191-207. https://doi.org/10.1017/S1473550411000012
18. Montero-Calasanz M, Goker M, Potter G, Rohde M, Sproer C, Schumann P, et al. Geodermatophilus arenarius sp. nov., a xerophilic actinomycete isolated from Saharan desert sand in Chad. Extremophiles. 2012; 16(6): 903-909. https://doi.org/10.1007/s00792-012-0486-4
19. Mohammadipanah F, Wink J. Actinobacteria from arid and desert habitats: diversity and biological activity. Frontiers in microbiology. 2016; 6: 1541. https://doi.org/10.3389/fmicb.2015.01541
20. Kurapova A, Zenova G, Sudnitsyn I. Thermotolerant and thermophilic actinomycetes from soils of Mongolia desert steppe zone,Microbiology. 2012;81: 98-108. https://doi.org/10.1134/S0026261712010092
21. Taketani R, Kavamura V, Dos Santos S. Diversity and Technological Aspects of Microorganisms from Semiarid Environments. Diversity and Benefits of Microorganisms from the Tropics. 2017: 3-19. https://link.springer.com/chapter/10.1007/978-3-319-55804-2_1
22. Cowan DA, Hopkins DW, Jones BE, Maggs-Kölling G, Majewska R, Ramond J-B. Microbiomics of Namib Desert habitats. Extremophiles [Internet]. 2020;24(1):17–29. https://doi.org/10.1007/s00792-019-01122-7
23. Belov A, Cheptsov V, Vorobyova EA, Manucharova NA. Culturable Bacterial Communities Isolated from Cryo-Arid Soils : Phylogenetic Culturable Bacterial Communities Isolated from Cryo-Arid Soils : Phylogenetic and Physiological Characteristics. 2020;54(8): 95-104. http://dx.doi.org/10.1134/S0031030120080043
24. Lombana A. Suelos Colombianos: Una mirada desde la academia. Bogotá: Universidad Jorge Tadeo Lozano; 2004. https://www.utadeo.edu.co/es/publicacion/libro/editorial/235/suelos-colombianos-una-mirada-desde-la-academia
25. Florez M, Parra L, Jaramillo D, Jaramillo J. Paleosuelos del mioceno en el desierto de la tatacoa. Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales. 2013; 37(143): 229-244. https://doi.org/10.18257/raccefyn.6
26. Méndez YN. Identificación de bacterias nativas del desierto de Candelaria y Tatacoa (Colombia), sometidas a simulación de la radiación UV de Marte. Bogotá: Departamento de Ciencias Biológicas Universidad de los Andes; 2013. http://biblioteca.uniandes.edu.co/acepto22013220.php?id=3425.pdf
27. Guerrero J. Stratigraphy and sedimentary environments of the Honda Group in the La Venta area. Miocene uplift of the Colombian Andes. En: Kay R, Madden R, Cifelli R, Flynn J. A history of Neotropical Fauna: Vertebrate Paleobiology of the Miocene of Tropical South America,, Washington D.C: Smithsonian Institution Press; 1994. p. 592. https://www.researchgate.net/publication/313608799_Stratigraphy_Sedimentary_Environments_and_the_Miocene_Uplift_of_the_Colombian_Andes
28. Hermelin M. The Tatacoa Desert. En: Hermelin M. Landscapes and landforms of Colombia. New York: Springer; 2016. p. 127-138. https://www.springer.com/de/book/9783319117997
29. Goodfellow M, Peter K, Busse H, Trujillo M, Ludwig W, Suzuki K. Part A. Bergey’s Manual of Systematic Bacteriology: Volume 5: The Actinobacteria. New York: Springer; 2012. https://www.springer.com/gp/book/9780387950433
30. Winn W, Koneman E, Koneman W, Procop E, Schreckenberger G, Woods P, et al. Koneman diagnostico microbiológico: texto y atlas en color. Bogotá D.C.: Editorial Médica Panamericana; 2008. https://books.google.com.co/books/about/Koneman_Diagnostico_Microbiologico_Micro.html?hl=es&id=jyVQueKro88C&redir_esc=y
31. Carroll K, Jorgensen J, Pfaller M. Manual of Clinical Microbiology, Washington D.C.: ASM Press; 2015. https://www.asmscience.org/content/book/10.1128/9781555817381
32. Bull A, Asenjo J. Microbiology of hyper-arid environments: recent insights from the Atacama Desert, Chile. Antonie Van Leeuwenhoek. 2013; 103(6): 1173-1179. https://doi.org/10.1007/s10482-013-9911-7
33. Santos SN, Gacesa R, Taketani RG, Long PF, Melo IS. Genome Sequence of Streptomyces caatingaensis CMAA 1322, a New Abiotic Stress-Tolerant Actinomycete Isolated from Dried Lake Bed Sediment in the Brazilian Caatinga Biome. Genome announcements. 2015; 3(5): 1015-1020. https://mra.asm.org/content/3/5/e01020-15.short
34. Stevenson A, Hallsworth J. Water and temperature relations of soil actinobacteria. Environmental microbiology reports. 2014; 6(6): 744-755. https://doi.org/10.1128/genomea.01020-15
35. Zvyagintsev D, Zenova G, Doroshenko E, Gryadunova A, Gracheva T, Sudnitsyn I. Actinomycete growth in conditions of low moisture. Biology Bulletin. 2007; 34(3): 242-247. https://doi.org/10.1134/S1062359007030053
36. Anandan R, Dharumadurai D, Manogaran GP. An introduction to actinobacteria. En: Dharumadurai D, Jiang Y. Actinobacteria-Basics and Biotechnological Applications. InTech; 2016. https://www.intechopen.com/books/actinobacteria-basics-and-biotechnological-applications/an-introduction-to-actinobacteria
37. Adhya TK, Kumar N, Reddy G, Podile AR, Bee H, Samantaray B. Microbial mobilization of soil phosphorus and sustainable P management in agricultural soils. Curr Sci. 2015;108:1280–1287. https://www.jstor.org/stable/24905489
38. Ávila I, Rodríguez M, Franco M, Pedroza A, Gutierrez I. Use of agricultural wastes for biomass production of the plant growth promoter actinobacteria, streptomyces sp. mcr26. Journal of Experimental Biology and Agricultural Sciences. 2014;2(5): 460- 472. https://www.intechopen.com/books/actinobacteria-basics-and-biotechnological-applications/an-introduction-to-actinobacteria
39. Sivaraman G, Siva V. Microbiological spoilage of dried dishes. SSRN. 2015;2709070: 1-5. https://dx.doi.org/10.2139/ssrn.2709070
40. Dilbaghi N, Sharma S. Food spoilage, food infections and intoxications caused by microorganisms and methods for their detection. 2007. https://www.semanticscholar.org/paper/Food-spoilage%2C-food-infections-and-intoxications-by-Dilbaghi-Sharma/bf1979600620a-165f83e3e27433c6121531aeb9f
41. Hocking A, Isolation and identication of xerophilic fungi in stored commodities. En: Champ B, Highley E, Hocking A, Pitt J. Fungi and Mycotoxins in Stored Products, Canberra: Australian Centre for International Agricultural Research; 1991. p. 266. https://ageconsearch.umn.edu/record/134649/files/PR036.pdf#page=62
42. Nicolaus B, Manca M, Lama L, Esposito E, Gambacorta A. Lipid modulation by environmental stresses in two models of extremophiles isolated from Antarctica. Polar Biology. 2001; 24(1): 1-8. https://doi.org/10.1007/s003000000156
43. Satyanarayana T, Raghukumar C, Shivaji S. Extremophilic microbes: Diversity and perspectives. Current Science. 2005: 78-90. https://www.researchgate.net/publication/298223942_Extremophilic_microbes_Diversity_and_perspectives
44. Fredsgaard C, Moore D, Al Soudi D, Crisler D, Chen F, Clark B, et al. Relationships between sucretolerance and salinotolerance in bacteria from hypersaline environments and their implications for the exploration of mars and the icy worlds. International Journal of Astrobiology.2016: 1-7. https://doi.org/10.1017/S1473550416000240
45. Rillig M, Lehmann A, Aguilar-Trigueros CA, Antonovics J, Caruso T, Hempel S, et al. J. Soil microbes and community coalescence. Pedobiología. 2016; 59(1): 37-40. https://doi.org/10.1016/j.pedobi.2016.01.001
46. Tse C, Ma K. Growth and metabolism of extremophilic microorganisms. En: P. Rampelotto. Biotechnology of Extremophiles. Springer; 2016. p. 1-46. https://link.springer.com/chapter/10.1007/978-3-319-13521-2_1
47. Huang W, Ertekin E, Wang T, Cruz L, Dailey M, DiRuggiero J, et al. Mechanism of water extraction from gypsum rock by desert colonizing microorganisms. Proc Natl Acad Sci U S A. 2020;117(20):10681–10687. https://doi.org/10.1073/pnas.2001613117
48. Narvaez-Reinaldo J, Barba I, Gonzalez-Lopez J, Tunnacliffe MM. Rapid method for isolation of desiccation-tolerant strains and xeroprotectants. Applied and environmental microbiology. 2010; 76(15): 5254-5262. https://aem.asm.org/content/76/15/5254
49. Vilchez S, Manzanera M. Biotechnological uses of desiccation-tolerant microorganisms for the rhizoremediation of soils subjected to seasonal drought. Applied microbiology and biotechnology. 2011; 91(5): 1297-1304. https://doi.org/10.1007/s00253-011-3461-6
50. Chen G, XR J. Next generation industrial biotechnology based on extremophilic bacteria. Current opinion in biotechnology. 2018; 50(94): 94-100. https://doi.org/10.1016/j.copbio.2017.11.016