Inespecificidad persistente en amplificación isotérmica mediada por loop para diagnóstico de malaria

Persistent non-specificity in loop-mediated insothermal amplication for malaria diagnosis

Publicado 2016-12-31

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Vol. 14 Núm. 26 (2016)
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Artículo Original Producto de Investigación
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Pachón Meza, L. C., & Barrera Ayala, S. M. (2016). Inespecificidad persistente en amplificación isotérmica mediada por loop para diagnóstico de malaria. REVISTA NOVA , 14(26), 29-34. https://doi.org/10.22490/24629448.1748
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https://doi.org/10.22490/24629448.1748
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Licencia Creative Commons
NOVA por http://www.unicolmayor.edu.co/publicaciones/index.php/nova se distribuye bajo una Licencia Creative Commons Atribución-NoComercial-SinDerivar 4.0 Internacional.

Así mismo,  los autores mantienen sus derechos de propiedad intelectual sobre los artículos.  

Laura Cristina Pachón Meza
Instituto Nacional de Salud
    Sandra Milena Barrera Ayala
    Instituto Nacional de Salud
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      Objetivo. Describir el ensayo realizado para la estandarización de la técnica de diagnóstico para malaria, denominada amplificación isotérmica mediada por loop (LAMP). Métodos. Se extrajeron 48 muestras divididas en 22 muestras para P. falciparum, 20 de P. vivax y 6 negativas para las mismas especies. Resultados. Se observaron falsos positivos, amplificaciones específicas que evidencian la inespecificidad de la técnica. Se propone estudiar los amplicones obtenidos por medio de restricción de fragmentos y secuenciación para elucidar el origen del problema.

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      34. ==========================================
      35. DOI: http://dx.doi.org/10.22490/24629448.1748
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