Evaluation of the genetic stability of Leptospira reference strains maintained under two conservation methods

Evaluation of the genetic stability of Leptospira reference strains maintained under two conservation methods

Publicado 2022-12-19

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Vol. 20 Núm. 39 (2022): Julio - Diciembre
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Patiño Burbano, R. E., Beltran, O. G., Torres Higuera, L. D., & Rodríguez Bautista, J. L. (2022). Evaluation of the genetic stability of Leptospira reference strains maintained under two conservation methods. REVISTA NOVA , 20(39), 65-79. https://doi.org/10.22490/24629448.6585
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https://doi.org/10.22490/24629448.6585
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NOVA por http://www.unicolmayor.edu.co/publicaciones/index.php/nova se distribuye bajo una Licencia Creative Commons Atribución-NoComercial-SinDerivar 4.0 Internacional.

Así mismo,  los autores mantienen sus derechos de propiedad intelectual sobre los artículos.  

Rocio Esperanza Patiño Burbano
Corporación colombiana de investigación agropecuaria AGROSAVIA
    https://orcid.org/0000-0003-2752-2434

    Oscar Gabriel Beltran
    Corporación colombiana de investigación agropecuaria AGROSAVIA
      https://orcid.org/0000-0002-1026-7177

      Ligia Denise Torres Higuera
      Corporación colombiana de investigación agropecuaria AGROSAVIA
        https://orcid.org/0000-0002-2731-6529

        Jose Luis Rodríguez Bautista
        Universidade Federal Rural do Rio de Janeiro
          https://orcid.org/0000-0002-9407-171X

          Mostrar biografía de los autores

          Las metodologías para el mantenimiento de cepas de referencia de Leptospira spp., requieren técnicas de conservación capaces de garantizar la viabilidad, evitar la contaminación y mantener las características genéticas originales de las cepas. La diversidad en los procesos de reorganización genética de Leptospira spp., hacen necesario el desarrollo de metodologías moleculares que permitan el seguimiento de la estabilidad genética de las cepas, bajo los diferentes métodos de conservación. Este estudio determinó el grado de conservación de los genes 16S rRNA y ompL1 de 10 serovares de referencia de la colección de Leptospira spp., pertenecientes al Sistema de Bancos de Germoplasma de la nación para la Alimentación y la Agricultura (SBGNAA); lo que permitió corroborar que estos no han sufrido cambios considerables, puesto que se evidenciaron similitudes superiores al 99,69% para 16S rRNA y de 99,02% para ompL1, en los alineamientos pareados, lo que comprueba la estabilidad genética y la pureza de las cepas de referencia de Leptospira spp., mantenidas en criopreservación en nitrógeno líquido a -196 °C y a temperatura ambiente durante ocho años aproximadamente

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