Determinación de metabolitos secundarios a partir de Bacillus subtilis efecto biocontrolador sobre Fusarium sp.
Artículo barra lateral
PDF
FLIP
HTML
Sección: Artículo Original
Cómo citar
Ariza, Y. (2012). Determinación de metabolitos secundarios a partir de Bacillus subtilis efecto biocontrolador sobre Fusarium sp. NOVA, 10(18). https://doi.org/10.22490/24629448.1003
Formatos de citación
Contenido principal del artículo
Autores
Yesid Ariza
Resumen
El uso de agentes de control biológico es una alternativa eficaz para el tratamiento de las enfermedades en plantas, contribuyendo en la disminución del uso de pesticidas químicos. Microorganismos del género Bacillus se han convertido en el centro de interés por su producción de metabolitos de tipo secundario, con propiedades antifúngicas, supresores efectivos contra diversos Fitopatógenos como Fusarium, Pythium, Phytophthora y Rhizoctonia. El objetivo del presente estudio fue identificar la presencia de metabolitos secundarios obtenidos por fermentación en estado líquido a partir de una cepa de Bacillus subtilis, los cuales fueron analizados mediante cromatografía de alta resolución (HPLC). Se confirmó su efecto biocontrolador sobre Fusarium sp por pruebas de excavación en placa. Se identificó el antibiótico Iturina A, con una concentración aproximada de 151.805 mg/l y las pruebas de antagonismo in vitro expresaron un porcentaje de 70 a 100% de inhibición de crecimiento sobre Fusarium sp. Se espera a futuro identificar la presencia de otros metabolitos de tipo secundario, de importancia en fitosanidad, como la producción de surfactina y fengicina.
Palabras clave:
Detalles del artículo
Licencia
NOVA por http://www.unicolmayor.edu.co/publicaciones/index.php/nova se distribuye bajo una Licencia Creative Commons Atribución-NoComercial-SinDerivar 4.0 Internacional.
Así mismo, los autores mantienen sus derechos de propiedad intelectual sobre los artículos.
Referencias
1. S. Mizumoto . M. Hirai . M. Shoda. Production of lipopeptide antibiotic iturin A using soybean curd residue cultivated with Bacillus subtilis in solid-state fermentation. Appl Microbiol Biotechnol. 2006; 72: 869–875.
2. Naorska, K., Bikowski, M. Multicellular behaviour and production of a wide variety of toxic substances support usage of Bacillus subtilis as a powerful biocontrol agent. Acta Biochimica
Polonica. 2007; 54: 495-508.
3. Bais, H.P., Fall, R., Vivanco, J.M., Biocontrol of Bacillus subtilis against infection of Arabidopsis roots by Pseudomonas syringae is facilitated by biofilm formation and surfactin production. Plant Physiology. 2004; 134: 307–319.
4. Muaaz Mutaz Al-Ajlani, Muhammad Abid Sheikh, Zeeshan Ahmad and Shahida Hasnain. Production of surfactin from Bacillus subtilis MZ-7 grown on pharmamedia commercial medium. Microbial Cell Factories. 2007; 6:17.
5. Rodrigues, L.; Banat, I.M.; Teixeira, J.; Oliveira, R. Biosurfactants: potential applications in medicine. J. Antimicrob. Chemother. 2006; 57: 609–618.
6. Mireles, J.R.; Toguchi, A.; Harshey, R.M. Salmonella enterica serovar typhimurium swarming mutants with altered biofilmforming abilities: surfactin inhibits biofilm formation. J. Bacteriol.
2001; 183: 5848–5854.
7. Niran Roongsawang, Kenji Washio and Masaaki Morikawa. Review Diversity of Nonribosomal Peptide Synthetases Involved in the Biosynthesis of Lipopeptide Biosurfactants, International Journal of Molecular Sciences ISSN 1422-0067, Int. J. Mol. Sci. 2011; 12: 141-172.
8. Roongsawang, N.; Thaniyavarn, J.; Thaniyavarn, S.; Kameyama, T.; Haruki, M.; Imanaka, T.; Morikawa, M.; Kanaya, S. Isolation and characterization of a halotolerant Bacillus subtilis BBK-1 which produces three kinds of lipopeptides: bacillomycin L, plipastatin, and surfactin. Extremophiles. 2002; 6: 499–506.
9. Isogai, I.; Takayama, S.; Murakoshi, S.; Suzuki, A. Structure of β-amino acids in antibiotics iturin A. Tetrahedron Lett. 1982, 23: 3065–3068.
10. Leclere, V.; Marti, R.; Bechet, M.; Fickers, P.; Jacques, P. The lipopeptides mycosubtilin and surfactin enhance spreading of Bacillus subtilis strains by their surface-active properties. Arch.
Microbiol. 2006; 186: 475–483.
11. Moyne, A.L.; Cleveland, T.E.; Tuzun, S. Molecular characterization and analysis of the operon encoding the antifungal lipopeptide bacillomycin D. FEMS Microbiol. Lett. 2004; 234: 43–49.
12. Tsuge, K.; Akiyama, T.; Shoda, M. Cloning, sequencing, and characterization of the iturin A operon. J. Bacteriol. 2001, 183, 6265–6273.
13. Hansen, D.B.; Bumpus, S.B.; Aron, Z.D.; Kelleher, N.L.; Walsh, C.T. The loading module of mycosubtilin: an adenylation domain with fatty acid selectivity. J. Am. Chem. Soc. 2007; 129: 6366–6367.
14. Wu, C.Y.; Chen, C.L.; Lee, Y.H.; Cheng, Y.C.; Wu, Y.C.; Shu, H.Y.; Gotz, F.; Liu, S.T. Nonribosomal synthesis of fengycin on an enzyme complex formed by fengycin synthetases. J.
Biol. Chem. 2007, 282: 5608–5616.
15. Gonzalez, M., I. Torres y H. Guzman. 2002. Búsqueda de resistencia natural contra patógenos de raíz Phytophthora capsici, Fusarium solani y Fusarium oxysporum en colectas de Chile. Proceedings of the 16th Internacional Pepper Conference. Tampico y Tamaulipas, México.
16. Juan Guillermo Cubillos Hinojosa1; Alberto Paez Redondo y Lauris Mejia Doria. Evaluation of the Biocontrol Capacity of Trichoderma harzianum Rifai against Fusarium solani (Mart.) Sacc. Associated to the Complex “Dryer” in Passion Fruit Under Greenhouse Conditions. Int. J. Mol. Sci. 2010; 11: 4526-4538.
17. Sá nchez L, Corrales L, Lancheros A. Evaluación in vivo de potencial biocontrolador de morfotipos bacterianos nativos contra fusarium oxysporum y caracterización por PCR de los microorganismos mas eficientes. Grupo Ceparium UCMC. 2008. Literatura gris.
18. Mitidieri, L. Control Biologico de hongos del suelo con Trichoderma. IDIA. 1988; 44:45-49.
19. Whipps, JM. 1987. Effect of media of growth and interactions between a range of soil-borne glass-house pathogens and antagonistic fungi. New Phytol.,107: 127-142.
20. S. Mizumoto & M. Hirai & M. Shoda. Enhanced iturin A production by Bacillus subtilis and its effect on suppression of the plant pathogen Rhizoctonia solani. Appl Microbiol Biotechnol (2007) 75:1267–1274.
21. Georgiou, G.; Lin, S.C.; Sharma, M.M. Surface-active compounds from microorganisms. Biotechnology (NY) 1992, 10: 60–65.
22. Arima, K.; Kakinuma, A.; Tamura, G. Surfactin, a crystalline peptidelipid surfactant produced by Bacillus subtilis: isolation, characterization and its inhibition of fibrin clot formation. Biochem. Biophys. Res. Commun. 1968; 31: 488–494.
23. Eppelmann, K.; Stachelhaus, T.; Marahiel, M.A. Exploitation of the selectivity-conferring code of nonribosomal peptide synthetases for the rational design of novel peptide antibiotics. Biochemistry 2002, 41: 9718–9726.
24. Kim, P.I.; Ryu, J.; Kim, Y.H.; Chi, Y.T. Production of biosurfactant lipopeptides iturin A, fengycin and surfactin A from Bacillus subtilis CMB32 for control of Colletotrichum gloeosporioides. J. Microbiol. Biotechnol. 2010; 20: 138–145.
25. Maget-Dana R, Peypoux F. Iturins, a special class of poreforming lipopeptides: biological and physicochemical properties. Toxicology. 1994;87: 151–174.
26. Mohammad Shahedur Rahman∗, Takashi Ano, Makoto Shoda, Second stage production of iturin A by induced germination of Bacillus subtilis RB14, Journal of Biotechnology. 2006;125:513–515.
27. Francois Coutte & Didier Lecouturier, Production of surfactin and fengycin by Bacillus subtilis in a bubbleless membrane bioreactor, Appl Microbiol Biotechnol. 2010; 87:499–507.
28. Jing Li, Qian Yang, Li-hua Zhao, Shu-mei Zhang, Yu-xia Wang, and Xiao-yu Zhao. Purification and characterization of a novel antifungal protein from Bacillus subtilis strain B29. J Zhejiang Univ Sci B. 2009; 10(4): 264–272.
-----------------------------------------------------------------------------------
DOI: http://dx.doi.org/10.22490/24629448.1003
2. Naorska, K., Bikowski, M. Multicellular behaviour and production of a wide variety of toxic substances support usage of Bacillus subtilis as a powerful biocontrol agent. Acta Biochimica
Polonica. 2007; 54: 495-508.
3. Bais, H.P., Fall, R., Vivanco, J.M., Biocontrol of Bacillus subtilis against infection of Arabidopsis roots by Pseudomonas syringae is facilitated by biofilm formation and surfactin production. Plant Physiology. 2004; 134: 307–319.
4. Muaaz Mutaz Al-Ajlani, Muhammad Abid Sheikh, Zeeshan Ahmad and Shahida Hasnain. Production of surfactin from Bacillus subtilis MZ-7 grown on pharmamedia commercial medium. Microbial Cell Factories. 2007; 6:17.
5. Rodrigues, L.; Banat, I.M.; Teixeira, J.; Oliveira, R. Biosurfactants: potential applications in medicine. J. Antimicrob. Chemother. 2006; 57: 609–618.
6. Mireles, J.R.; Toguchi, A.; Harshey, R.M. Salmonella enterica serovar typhimurium swarming mutants with altered biofilmforming abilities: surfactin inhibits biofilm formation. J. Bacteriol.
2001; 183: 5848–5854.
7. Niran Roongsawang, Kenji Washio and Masaaki Morikawa. Review Diversity of Nonribosomal Peptide Synthetases Involved in the Biosynthesis of Lipopeptide Biosurfactants, International Journal of Molecular Sciences ISSN 1422-0067, Int. J. Mol. Sci. 2011; 12: 141-172.
8. Roongsawang, N.; Thaniyavarn, J.; Thaniyavarn, S.; Kameyama, T.; Haruki, M.; Imanaka, T.; Morikawa, M.; Kanaya, S. Isolation and characterization of a halotolerant Bacillus subtilis BBK-1 which produces three kinds of lipopeptides: bacillomycin L, plipastatin, and surfactin. Extremophiles. 2002; 6: 499–506.
9. Isogai, I.; Takayama, S.; Murakoshi, S.; Suzuki, A. Structure of β-amino acids in antibiotics iturin A. Tetrahedron Lett. 1982, 23: 3065–3068.
10. Leclere, V.; Marti, R.; Bechet, M.; Fickers, P.; Jacques, P. The lipopeptides mycosubtilin and surfactin enhance spreading of Bacillus subtilis strains by their surface-active properties. Arch.
Microbiol. 2006; 186: 475–483.
11. Moyne, A.L.; Cleveland, T.E.; Tuzun, S. Molecular characterization and analysis of the operon encoding the antifungal lipopeptide bacillomycin D. FEMS Microbiol. Lett. 2004; 234: 43–49.
12. Tsuge, K.; Akiyama, T.; Shoda, M. Cloning, sequencing, and characterization of the iturin A operon. J. Bacteriol. 2001, 183, 6265–6273.
13. Hansen, D.B.; Bumpus, S.B.; Aron, Z.D.; Kelleher, N.L.; Walsh, C.T. The loading module of mycosubtilin: an adenylation domain with fatty acid selectivity. J. Am. Chem. Soc. 2007; 129: 6366–6367.
14. Wu, C.Y.; Chen, C.L.; Lee, Y.H.; Cheng, Y.C.; Wu, Y.C.; Shu, H.Y.; Gotz, F.; Liu, S.T. Nonribosomal synthesis of fengycin on an enzyme complex formed by fengycin synthetases. J.
Biol. Chem. 2007, 282: 5608–5616.
15. Gonzalez, M., I. Torres y H. Guzman. 2002. Búsqueda de resistencia natural contra patógenos de raíz Phytophthora capsici, Fusarium solani y Fusarium oxysporum en colectas de Chile. Proceedings of the 16th Internacional Pepper Conference. Tampico y Tamaulipas, México.
16. Juan Guillermo Cubillos Hinojosa1; Alberto Paez Redondo y Lauris Mejia Doria. Evaluation of the Biocontrol Capacity of Trichoderma harzianum Rifai against Fusarium solani (Mart.) Sacc. Associated to the Complex “Dryer” in Passion Fruit Under Greenhouse Conditions. Int. J. Mol. Sci. 2010; 11: 4526-4538.
17. Sá nchez L, Corrales L, Lancheros A. Evaluación in vivo de potencial biocontrolador de morfotipos bacterianos nativos contra fusarium oxysporum y caracterización por PCR de los microorganismos mas eficientes. Grupo Ceparium UCMC. 2008. Literatura gris.
18. Mitidieri, L. Control Biologico de hongos del suelo con Trichoderma. IDIA. 1988; 44:45-49.
19. Whipps, JM. 1987. Effect of media of growth and interactions between a range of soil-borne glass-house pathogens and antagonistic fungi. New Phytol.,107: 127-142.
20. S. Mizumoto & M. Hirai & M. Shoda. Enhanced iturin A production by Bacillus subtilis and its effect on suppression of the plant pathogen Rhizoctonia solani. Appl Microbiol Biotechnol (2007) 75:1267–1274.
21. Georgiou, G.; Lin, S.C.; Sharma, M.M. Surface-active compounds from microorganisms. Biotechnology (NY) 1992, 10: 60–65.
22. Arima, K.; Kakinuma, A.; Tamura, G. Surfactin, a crystalline peptidelipid surfactant produced by Bacillus subtilis: isolation, characterization and its inhibition of fibrin clot formation. Biochem. Biophys. Res. Commun. 1968; 31: 488–494.
23. Eppelmann, K.; Stachelhaus, T.; Marahiel, M.A. Exploitation of the selectivity-conferring code of nonribosomal peptide synthetases for the rational design of novel peptide antibiotics. Biochemistry 2002, 41: 9718–9726.
24. Kim, P.I.; Ryu, J.; Kim, Y.H.; Chi, Y.T. Production of biosurfactant lipopeptides iturin A, fengycin and surfactin A from Bacillus subtilis CMB32 for control of Colletotrichum gloeosporioides. J. Microbiol. Biotechnol. 2010; 20: 138–145.
25. Maget-Dana R, Peypoux F. Iturins, a special class of poreforming lipopeptides: biological and physicochemical properties. Toxicology. 1994;87: 151–174.
26. Mohammad Shahedur Rahman∗, Takashi Ano, Makoto Shoda, Second stage production of iturin A by induced germination of Bacillus subtilis RB14, Journal of Biotechnology. 2006;125:513–515.
27. Francois Coutte & Didier Lecouturier, Production of surfactin and fengycin by Bacillus subtilis in a bubbleless membrane bioreactor, Appl Microbiol Biotechnol. 2010; 87:499–507.
28. Jing Li, Qian Yang, Li-hua Zhao, Shu-mei Zhang, Yu-xia Wang, and Xiao-yu Zhao. Purification and characterization of a novel antifungal protein from Bacillus subtilis strain B29. J Zhejiang Univ Sci B. 2009; 10(4): 264–272.
-----------------------------------------------------------------------------------
DOI: http://dx.doi.org/10.22490/24629448.1003
Descargas
La descarga de datos todavía no está disponible.
Horario: Lunes a Viernes: 8:00 a.m. a 5:00 p.m
