Efecto antimicrobiano In vitro de propóleos argentinos, colombianos y cubano sobre Streptococcus mutans ATCC 25175

Efecto antimicrobiano In vitro de propóleos argentinos, colombianos y cubano sobre Streptococcus mutans ATCC 25175

It was evaluated the antimicrobial activity of four propolis extracts from Argentina, five from Colombia and onefrom Cuba, using Streptococcus mutans ATCC 25175. The samples were kept in a 96% ethylic alcohol solution,remaining a concentration of 3%. The bactericidal and bacteriostatic activity was measured by the minimun inhibitoryconcentration among a range of 0.02 and 15mg/ml. All of the analyzed samples showed activity against StreptococcusMutans when they are used to concentrations 15 a 3.75 mg/ml.

The propolis that presented the highest effect bactericidal was number 2 and 3 (sample from Colombia) after 48hours of incubation. The highest effect bacteriostatic was presented by the sample 2 (propolis from Colombia) after24 hours of incubation. 70% of the propóleo samples increased their activity after a time of incubation of 48 hourscompare to the effect shown to the 24 hours.

All the Colombian samples showed a superior effect, when the bacteria were exposed to the propóleo for alonger period of time; in two of these cases until of five dilutions, samples 2 and 3, this effect was presented in amoderate way, using the argentine samples 6 and 10 while the other samples (30%) remained steady.

1. T anzer J, Livingston J, Thompson A. The Microbiology of Primary Dental Caries in Humans. J Dent Educ. 2001;65:1028-1037.
2. Ministerio de Salud. Galviz RV. III Estudio Nacional de Salud Bucal - ENSAB III, II Estudio Nacional de Factores de Riesgo de Enfermedades Crónicas. ENFRECII; Tomo VII Estudio Nacional de Salud Bucal, Colombia 1999.
3. Martínez T, Figueroa J, Martinez N. Actividad antimicrobiana de muestras de propóleo Colombiano. Espectro antimicrobiano del propóleo proveniente de apiarios de la zona norte del Valle del Cauca y Sur del Quindío. En Trabajo de grado Facultad de Medicina Veterinaria y de Zootecnia Universidad Nacional de Colombia, 2003.
4. Farré R, Frasquet I, Sánchez A. El propolis y la salud. Ars Pharama. 2004;45:21-43.
5. Dobrowolski J, Vohora S, Sharma K, Shah S, Naqvi S, Dandiya P. Antibacterial, antifungal, antiamoebic, anti-inflammatory and antipyretic studies on propolis bee products. J Ethnopharmacol.1991;35:77-82.
6. Koo H, Rosalen P, Cury J, Park Y, Bowen W. Effects of compounds found in propolis on Streptococcus mutans growth and on glucosyltransferase activity. Antimicrob Agents Chemother. 2002;46:1302-1309.
7. Duarte S. Rosalen P, Hayacibara M, Cury J, Bowen W, Marquis R, Rehder V, Sartoratto A, Ikegaki M, Koo H. The influence of a novel propolis on mutans streptococci biofilms and caries development in rats. Arch Oral Biol. 2006;51:15-22.
8. Pinto M, Rodríguez MC, Median R. Prueba “in vitro” del líquido de la cáscara de la nuez de marañon (Anacardium occidentale) en el Streptococcus mutans. Trabajo de Pregrado Facultad de Odontología Bogotá D.C. 1993, Distinción meritoria. Mención de honor XI Encuentro de Investigación en Odontología ACFO Medellín 2000.
9. T anzer JM, Börjesson AC, Laskowski L, Testa M. Glucosesucrose-potassium tellurite-bacitracin agar, an alternative to mitis salivarius-bacitracin agar for enumeration of Streptococcus mutans. J Clin Microbiol. 1984;20:653-659.
10. Tolosa L, Cañizares E. Obtención, caracterización y evaluación de la actividad antimicrobiana de extractos de propóleos de Campeche. Ars Pharmac. 2002;43:187-204.
11. -----------------------------------------------------------------------------------
12. DOI: http://dx.doi.org/10.22490/24629448.376