Detection of dominants antigens to diagnosis of cisticercosis for immunoelectrotransference (EITB)

Detección de antígenos dominantes para el diagnóstico de cisticercosis por inmunoelectrotransferencia (Eitb)

It is employed lyophilized cyst of Cisticerco cellulosae to prepare antigenic extract of which it is used in the identification of Taenia solliumantigens recognized for IgG antibodies of patients with cisticercosis, for immunoelectrotransfer assay (EITB). It is analyze 193 samples (105 cerebrospinal fluid and 88 sera) from patients with diagnosis of cisticercosis for finding clinic, epidemiologic and inmunienzimatic assay (ELISA). The analysis of the antigenic proteins for electrofiretic, allowed to identify polypeptides of 29, 45, 66, 96 and 116 kDa. The frecuency greates in sera and LCR was 29, 45 and 66 kDa. In conclusion, this immunidominants, will power employ in epidemiologic studies and cisticercosis clinics for Western blot. 

---

Article visits 313 | PDF visits 111

---

Downloads

Download data is not yet available.

1. Sarti E. La teniosis y cisticercosis por Taenia solium. Revista de Salud Pública de México 1998; 39(3):1-13.
2. Pinilla G, Navarrete J, Almonacid C, Bermúdez M. Cisticercosis: zoonosis y sociedad; aspectos inmunológicos, de diagnóstico y control. Impreso universitario. Bogotá: Facultad de Ciencias de la Salud, Universidad Colegio Mayor de Cundinamarca; 2003.
3. Allan JC,Velásquez M, Torres R, et al. Field trial of diagnosis of Taenia solium taeniasis by coproantigen enzyme linked inmunosorbent assay. Am J Trop Med Hyg 1996;54:352-6.
4. Chapman A, Vallejo V, Mossie KG, et al. A. Isolation and characterization of species-specific DNA probes from Taenia soliumandTaenia saginata and their use in egg detection assay. J Clin Microbiol 1995;33(5):1283-8.
5. Pinilla G, Navarrete J, Serrano ML. Generaciones diagnósticas en cisticercosis: del microscopio a la biología molecular. Acovez 1998;23(2):20-4.
6. González LM. PCR tool for the differential diagnosis of Taenia saginataandTaenia soliumtaeniasis / cisticercosis from different geographical locations. Diagn Microbiol Infect Dis 2002;2 (4):243-9.
7. Feldman M, Plancarte A, Sandoval M, et al. Comparison of two assays (EIA and EITB) and two samples (saliva and serum) for the diagnosis of neurocysticercosis. Trans Roy Soc Trop Med Hyg 1990;84:559-62.
8. Sloan L, Schneider S, Rosenblatt J. Evaluation of enzyme-linked immnunoassay for serological diagnosis of cysticercosis. J of Clin Micro 1995;33:3124-8
9. Garcia H, Harrison LJ, Parkhouse RM, et al.Aspecific antigendetection ELISA for the diagnosis of human neurocysticercosis. The cysticercosis working group in Peru. Trans Soc Trop Med Hyg 1998;92(4):411-4.
10. Winograd E, Rojas A. Identification of two nonglycosylated polypetides of Taenia solium recognized by immunoglobulins from patients with neurocysticercosis. Parasitol Res 1999;85:513-7.
11. Bradford M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein- die binding. Anal Biochem 1976;72:248-54.
12. Laemmly YU. Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 1970;227:680-5.
13. Maniatis F, Sambrok J. Molecular Cloning Manual Laboratory. 2ª ed. New York: Col Spring Harbor Laboratory Press; 1989.
14. Pinilla G, Navarrete J, Almonacid C, Bermúdez M. Análisis de métodos de revelado para electroforesis PAGE-SDS y western blot en el diagnóstico de cisticercosis. Bogotá: Universidad Colegio Mayor de Cundinamarca, Facultad de Ciencias de la Salud, Programa de Bacteriología; 1999.
15. Flisser A, Williams K, Laclette JP, et al. Biochemical and immunologycal characterization of antigen B purified from cysticerci of Taenia solium. In: Cysticercosis: Present state of knowledge and perspectives. NewYork: Academic Press; 1982. p. 437-51.
16. Tsang, V, Brand J, Boyer A. An Enzyme linked immonuelectrotransfer bot assay and glicoprotein antigens for diagnosis human cysticercosis (Taenia solium). J Infect Dis 1989;159(1):50-9.
17. Ev LV, Maia AA, Pianetti G, Nascimento N. Immunologycal evaluation of 26 kDa antigen from Taenia solium larvae for especific immunodiagnosis of human neurocysticercosis. Parasitol Res 1999;85(2):98-102.
18. Flisser A, Plancarte A, Correa D, et al. New approaches in the diagnosis of Taenia solium cysticercosis and taeniasis. Ann Parasitol Hum Comp 1990;65(Suppl 1):95-8.
19. Giraldo JC, Piragauta MA, Vargas C, et al. Valoración de la reactividad antigénica con sueros de porcino de las fracciones de 95, 45 y 36 kDa. del metacéstodo de Taenia solium. Revista Científica de UNIINCA 2001;7(1):23-33.
20. -------------------------------------------------------------------------------
21. DOI: http://dx.doi.org/10.22490/24629448.1053