Polimorfismos del sistema HLA (loci A*, B* y DRB1*) en población colombiana

Polimorfismos del sistema HLA (loci A*, B* y DRB1*) en población colombiana

The objective of this work was to determine the allelic frequencies of system HLA-A*, B* and DRB1* in asample of 981 individuals of the Colombian population. The sample size was purified from the data base of theColombian Network of transplantation from which 981 individuals were taken to apply the statistical analysis tothem by means of program ARLEQUIN version 2000.

A total diversity of 66 alleles was found. They represent 87% of world-wide allelic diversity level of loci A*, B*and DRB1*. 19 alleles for locus HLA-A*, 33 for locus HLA-B* and 14 for the DRB1* were found. Alleles HLA-A*02 and HLA-A*24 respectively displayed a frequency of 0.2696 and 0.2706, that correspond to 54% of theanalyzed sample. Alleles HLA-B*35 and HLA-B*51 were the most frequent of loci B* were 0,1926 and 0.1314,respectively. For the DRB1*, the DRB1*04 presents a high frequency solely: 0.238. The left over alleles showinferior frequencies 12%. The results of this work confirm, at a molecular level, the genetic diversity of the Colombianpopulation given that Colombia has 87% of polymorphisms HLA (loci A*, B* and DRB1*) found in the world.

1. MHC Sequencing Consortium. Complete sequence and gene map of a human major histocompatibility complex. MHC Sequencing Consortium. Nature. 1999;401:921-923.
2. Hardy H. Mendelian Proportions in a mixed population. Science. 1908;28:49-50.
3. Benjamin A, Bradley T. Pronostic assays for rejection and tolerance in organ transplantation. Transplant Immunol. 2005;14:193-201.
4. Ober C, Weitkamp L, Cox N, Dytch H, Kostyu D, Elias S. HLA and mate choice in humans. Am J. Hum. Genet. 1997;61:497-504.
5. Halpern S, Ubel P, Caplan A. Solid-organ trasplantation in HIV-infected patients. N Engl J Med. 2002;347:284-287.
6. www.ncbi.nlm.nih.gov/projects/dbmhc
7. Stefan S, Scheneider D, Excoffier L. Arlequin 2.000: a Software for population genetics data analysis. 2000. Genetics and Biometry Labs, University of Genera, Switzerland.
8. Matsuura M, Eguchi S. Estimation of gene frequency and test for Hardy-Weinberg Equilibrium in the HLA system. Environ Health Perspect. 1990;87:149-155.
9. Robinson J, Waller M, Parham P, Groot N, Bontrop R, Kennedy L, Stoehr P, Marsh S. E.IMGT/HLA and IMGT MHC: Sequence databases for the study of the major histocompatibility complex. Nucleic Acids Research. 2003;31:311-314.
10. Ossa H, Ramos O, Yunis E. Estudios genéticos de las comunidades indígenas del nororiente colombiano. Rev. Facultad de Medicina Universidad Nacional. 1994;42:9-16.
11. Olerup O, Zetterquist H. HLA-DR Typing by PCR amplification with sequence specific primer (PCR-SSP) In 2 hours: an alternative to serological DR typing in clinical practice including donor-recipient matching in cadaveric transplantations. Tissue Antigens. 1992;39:225-235.
12. Crespí C, Milà J, Martínez-Pomar N, Etxagibel A, Muñoz- Saa I, Priego D, Luque A, Pons J, Picornell A, Ramon M, Castro J, Matamoros N. HLA polymorphism in a Majorcan Population of Jewish Descent: Comparision with Majorca, Minorca, Ibiza (Balearic Islands) and other jewish communities. Tissue Antigens. 2002;60:282-291.
13. Garrigan D, Hendrick P. Perspective: Detecting adaptative molecular polymorphism: Lessons from de MHC. Evolution Int J Org Evolution. 2003;57: 1701-1722.
14. Bera, O, Cesaire R, Quelvennec E, HLA class I and class II allele and haplotype diversity in Martinicans, Tissue Antigens. 2001;57:200-207
15. De Pablo R, Beraun Y, Nieto A., Calzada J, Rementeria M, Sanz L, López-Nevot M, Martín J. - HLA class I and class II allele distribution in the Peruvian population. Tissue Antigens, 2000;56:507-514.
16. Cao K, Hollenbach J, Shi X, Shi W, Chopek M, Fernández-Viña MAAnalysis of the frequencies of HLA-A, B, and C alleles and haplotypes in the five major ethnic groups of the United States reveals high levels of diversity in these loci and contrasting distribution patterns in these populations. Hum Immunol. 200;62:1009-1030.
17. -----------------------------------------------------------------------------------
18. DOI: http://dx.doi.org/10.22490/24629448.369