Aislamiento e identificación de diez cepas bacterianas desnitrificantes a partir de un suelo agrícola contaminado con abonos nitrogenados proveniente de una finca productora de cebolla en la Laguna de Tota, Boyacá, Colombia

Aislamiento e identificación de diez cepas bacterianas desnitrificantes a partir de un suelo agrícola contaminado con abonos nitrogenados proveniente de una finca productora de cebolla en la Laguna de Tota, Boyacá, Colombia

This article presents the results of the adaptative versatility and tolerance of a bacterial consortium madeup of 10 bacterial stocks endemic to a contaminated soil coming from an onion farm located near Laguna deTota, Aquitania, Boyacá, Colombia. This soil has been exposed to the uncontrolled use of nitrogen rich fertilizers.The bacterial consortium from the soil is made up of Bacillus megaterium, Bacillus licheniformis,Pseudomonas stutzeri, Acinetobacter sp, Propionibacterium sp, Peptoestreptococcus sp, coagulasanegative sthaphylococci, Corynebacterium sp, Clostridium sp, and Actinomyces sp. It was demonstratedthat the isolated microorganisms have in vitro denitrifying capabilities, thus transforming nitrates to molecularnitrogen. The isolated bacterial association constitutes a bioremediation alternative to recover the soil that hasbeen contaminated by the excess of nitrogen rich fertilizers.

1. Çelen E, Akif M. Isolation and characterization of aerobic denitrifiers from agricultural soil. Turk Journal Biology 2003; 29: 9 -14.
2. Eweis J, Schroeder, E., Chang, D., Scow, K., Morton, R., and Caballero, R. Principios de Biorecuperación. Tratamientos para la descontaminación y regeneración de suelos y aguas subterráneas mediante procesos biológicos y fisicoquímicos. McGraw Hill. España. 1999. p. 131-147.
3. Rich J,Heichen P, Bottomley J, Cromack J. Community Composition and Functioning of Denitrifying Bacteria from Adjacent Meadow and Forest Soils. Applied and Environmental Microbiology. 2003;69(10): 5974-82.
4. Rösh C, Mergel A, Bothe H. Biodiversity of denitrifying and dinitrogen-fixing bacteria in an acid forest soil. Appl and Environmental Microbiol. 2002;3818-29.
5. Harting, E., and W. G. Zumft. Kinetics of nirS expression (cytochrome cd1 nitrite reductase) in Pseudomonas stutzeri during the transition from aerobic respiration to denitrification: evidence for a denitrification-specific nitrateand nitrite-responsive regulatory system. J. Bacteriol 2002; 181:161–166.
6. Sachiko Y, Naohiro N, Satoshi T, Akira H, Yubei H. Aerobic Denitrifyng Bacteria That Produce Low Levels of Nitrous Oxide. Applied and Enviromental Microbiology 2004;69(6):3152-3157.
7. Naoki T, Bernard M, Sakairi C, Sakaguchi Y, Kato I, Zhemin Z, Shoun H. Aerobic Denitrifying Bacteria That Produce Low Levels of Nitrous Oxide. Applied and Environmental Microbiology. 2003;69(6): 3152-57.
8. Kim H, Vanparys B, Wittebolle L, Verstraete W, Boon N, De Vos P. Cultivation of Denitrifying Bacteria: Optimization of isolation Conditions and Diversity Study. Applied an Enviromental Microbiology.2006.72(4)2637-2643.
9. Koneman EW. Diagnóstico microbiológico: texto y atlas color. 5 th ed Buenos Aires; 2001.
10. Denariaz G, Dayne W, Gall J. A halophilic denitrifier. Bacillus halophilic denitrifier sp. J.Syst.Bacteriol; 1989:39:145-151.
11. Sawer C. Chemestry For Enviromental Engineering. 1978.Mc.Graw-Hill.534p.
12. Braker W, Feseldt A, Witzel K. Develoment of PCR primer system amplification of nitrite reductase genes (NirK and nirS) to detect denitrifyng bacteria in environmental samples. Appl and Environ Microbiol. 1998; 3769-75.
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14. DOI: http://dx.doi.org/10.22490/24629448.360