Genotyfication and philogenetics analysis of Colombian isolated, the Denge Virus Type 2

Genotipificación y análisis filogenético de cepas colombianas del virus Dengue Tipo 2

Dengue virus is a flavivirus transmitted by the mosquito Aedes spp.and is the causative agent for an increasing public health problem in tropical areas over the world, with almost 3.000 million people at risk of infection. Dengue virus produces a broad spectrum of symptoms, varying from a mild flu-like illness called dengue fever, to a severe and some times fulminating haemorrhagic fever disease with shock. Genetic caracterization of distinct virus serotypes allow us to understand the epidemiological patterns of distribution as well as to demonstrate the presence of specific haemorrhagic strains causing very severe disease and death. In this report, we have determined the evolutionary origins of Dengue Virus Type 2 circulating in Colombia before and after the first  reported haemorrhagic fever case at the ends of 1989, by means of sequence and further analysis of a 240 pb PCR amplified fragment from the E/NS1 joining region; with the sequences obtained from 5 strains isolated before 1989 and 10 from strains isolated years after, a phylogenetic tree was constructed which showed the presence of 2 different genotypes in our country; comparison with strains previously isolated from around the world determined that one of the genotypes found corresponds to the native American genotype circulating before the appearance of haemorrhagic fever, while the later isolated strains of the second genotype belonged to a Southeast Asian genotype, suggesting displacement of the native strains by more virulent genotypes.

1. Organización Panamericana de la Salud. Dengue y Dengue Hemorrágico en las Américas: Guías para su Prevención y Control. Publicación científica 548; 1995.
2. Gubler DJ, Clark GG. Dengue/Dengue Haemorrhagic Fever: The Emergence of a Global Health Problem. Emerging Infect 1995;Dis 1:55-7.
3. Pan American Health Organization. Dengue and Dengue Haemorrhagic Fever in the Americas: An Overview of the Problem. Epidemiol Bull 1992;13:9-10.
4. Groot H. The Reinvasion of Colombia by Aedes aegypti: Aspects to Remember. Am J Trop Med Hyg 1980;29(3):330- 8.
5. Boshell J. El Dengue. Innovación y Ciencia 1995;4(5):46-50.
6. Halstead S. Antibody, Macrophages, Dengue Virus Infection, Shock and Haemorrhage:APathogenetic cascade. Rev Infect Dis 1989;11(s4):830-9.
7. Sabin AB. The Dengue Group of Viruses and its Family Relationships. Bacteriol Rev 1950;14:225-35.
8. HamonW, et al. Virus AssociatedWith Epidemic Hemorrhagic Fevers of the Philippines and Thailand. Science 1960;131:1102-3.
9. Rosen L. The Emperor‘s New Clothes Revisited, or Reflections on the Pathogenesis of Dengue Hemorrhagic Fever. Am J Trop Med Hyg 1977;26:337-43.
10. Rico-Hesse R. Molecular Evolution and Distribution of Dengue Viruses Type 1 and 2 in Nature. Virol 1990;174:479-93.
11. Rico-Hesse R, et al. Origins of Dengue Type 2 Viruses Associated With Increased Pathogenicity in the Americas. Virol 1997;230:244-51.
12. Chungue E, et al. Molecular Epidemiology of Dengue 3 Viruses and Genetic Relatedness Among Dengue 3 Strains Isolated From Patiens With Mild or Severe Form of Dengue Fever in Frensh Polynesia. J Gen Virol 1993;74:2765-70.
13. Chungue E, et al. Molecular Epidemiology of Dengue-1 and Dengue-4 Viruses. J Gen Virol 1995;76:1877-84.
14. Lanciotti R, et al. Molecular Evolution and Phylogeny of Dengue-4 Viruses. J Gen Virol 1997;78:2279-86.
15. Instituto Nacional de Salud, Subdirección de Epidemiología, 1991. Comunicación personal.
16. Ministerio de Salud e Instituto Nacional de Salud. Informe Quincenal Epidemiológico Nacional (IQEN). Bogotá: 1997 Oct 15; 2(19).
17. Ministerio de Salud e Instituto Nacional de Salud. Informe Quincenal Epidemiológico Nacional (IQEN). Bogotá: 1998
18. Oct 30; 3(20).
19. World Health Organization. Dengue in the Americas -An Up Date.Weekly Epidemiol Rec 1994;69(24):177-84.
20. Sariol CA, et al. Detection and genetic relationship of dengue virus sequences in seventeen-year-old paraffin-embedded samples from Cuba.Am J Trop Med Hyg 1999;61:994-1000.
21. Lanciotti R, et al. Rapid Detection and Typing of Dengue Viruses from Clinical Samples by Using Reverse Transcriptase-Polymerase Chain Reaction. J Clin Microbiol 1992;30:545-51.
22. Chomcczyski P, Sacci N. Single Step Method of RNA Isolation by Acid Guanidium-Thiocyanate-Phenol-Cloroform Extraction. Anal Bichem 1987;162:156-9.
23. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406-25.
24. RobinY, et al. Isolement Du Virus de Dengue au Senegal. Ann Virol (Inst. Pasteur) 1980;131 E:149-154.
25. World Health Organization. Dengue Haemorragic Fever in the Democratic Republic of Viet Nam. Dengue News l. SE AsianW. Fac. Regions, 1976;2:1-6.
26. Rudnick A. Studies of the ecology of Dengue in Malaysia: A Preliminary Report. J Med Ent 1965;2:203-8.
27. Zanotto PM, et al. Population Dynamics of Flaviviruses Revealed by Molecular Phylogenesis. Proc Nat Acad Sci USA 1996;93:548-53.
28. Trent DW, et al. Genetic Variation Among Dengue 2 Viruses of Different Geographic Origin. Virol 1983;128:271-84.
29. Rico-Hesse R, et al. Geographic Distribution of Wild Poliovirus Type 1 Genotipes. Virol 1987;160:311-2.
30. Oberste M, et al. Identification and Genetic Analysis of Panama-Genotype Venezuelan Equine Encephalitis Virus Subtype ID in Peru. Am J Trop Med Hyg 1998;58:41-6.
31. Rota P, et al. Molecular Epidemiology of Measles Virus. Virol 1995;6:379-86.
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33. DOI: http://dx.doi.org/10.22490/24629448.1057