Determination of adhesion factors associated with biofilm formation in clinical isolates of Staphylococcus aureus and Staphylococcus epidermidis

Determinación de factores de adhesión asociados a la formación de biopelícula en aislamientos clínicos de Staphylococcus aureus y Staphylococcus epidermidis

Published 2017-11-03

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Vol. 15 No. 27 (2017)
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Pinilla, G., Bautista, A., Cruz, C., Chavarro, B., Navarrete, J., Muñoz, L., & Gutiérrez, J. (2017). Determination of adhesion factors associated with biofilm formation in clinical isolates of Staphylococcus aureus and Staphylococcus epidermidis. NOVA, 15(27), 67-75. https://doi.org/10.22490/24629448.1959
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https://doi.org/10.22490/24629448.1959
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Gladys Pinilla
    Angela Bautista
      Claudia Cruz
        Bibiana Chavarro
          Jeannette Navarrete
            Liliana Muñoz
              Jennifer Gutiérrez
                Show authors biography
                Adhesion factors are virulence determinants that are expressed in microorganisms with the ability to form biofilms, contributing to the severity of nosocomial infections. Among these microbial surface components recognizing adhesive matrix molecules (MSCRAMMs), are fibronectin binding A and B (fnbA and B) clumping A and B (ClfA and B) and fibrinogen binding (Fib) factors. All of these have been described in Staphylococcus aureus and react with human extracellular matrix proteins. The goal of this study was to determine adhesion factors related to the biofilm formation in Staphylococcus. Method. For these purpose, 30 clinical isolates of Staphylococcus aureus and Staphylococcus epidermidis, from immunocompromised patients in three hospitals in Bogotá were characterized both, genotypically and phenotypically. The biofilm formation was determined through Congo Red and Violet Crystal and the genes FnbA, B, ClfA, B, Fib and operon ica ADBC were amplified through conventional and multiplex PCR. Results. Every clinical isolate were genotypically and phenotypically positive for the biofilm formation, being found the presence of the whole ica ADBC operon in 88,9%. The ClfA and ClfB were found by 70%; Fib 60%, fnbB 23% and 17% of fnbA. Conclusions. This study proved the presence of these virulence factorsin S. epidermidis, which so far have only been reported in S. aureus. This finding is important because it suggestes the relationship with horizontal gene transfer between these species, being the S. epidermidis an important genetic reservoir and a causal patobiont of nosocomial infections associated with medical devices.

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