Evaluación de las condiciones de crecimiento celular para la producción de astaxantina a patir de la microalga Haematococcus pluvialis

Clara Milena Niño Castillo; Francis Carolina Rodríguez Rivera; Luis Eduardo Díaz; Ana Graciela Lancheros Díaz

doi:10.22490/24629448.2073

Evaluation of Cell Growth Conditions for the Astaxanthin Production as of Haematococcus pluvialis Microalgae

Evaluación de las condiciones de crecimiento celular para la producción de astaxantina a patir de la microalga Haematococcus pluvialis

Published 2017-11-02

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Vol. 15 No. 28 (2017)

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Artículo Original

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Evaluation of Cell Growth Conditions for the Astaxanthin Production as of Haematococcus pluvialis Microalgae. (2017). NOVA, 15(28), 19-31. https://doi.org/10.22490/24629448.2073

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https://doi.org/10.22490/24629448.2073

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NOVA by http://www.unicolmayor.edu.co/publicaciones/index.php/nova is distributed under a license creative commons non comertial-atribution-withoutderive 4.0 international.

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Clara Milena Niño Castillo

Francis Carolina Rodríguez Rivera

Luis Eduardo Díaz

Ana Graciela Lancheros Díaz

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Haematococcus pluvialis

Astaxanthin

Cell growth

Culture media

Currently astaxanthin of natural origin is one of the carotenoids with important applications in the food, pharmaceutical and cosmetics, due to their large properties within its powerful antioxidant, cancer preventive effect, increase the immune response, inhibiting free radicals among many others. Haematococcus pluvialis is a freshwater green microalgae and is one of the largest natural sources of astaxanthin production as it is able to accumulate up to 3% astaxanthin by dry weight. The aim of this study was to determine the culture medium and the optimal conditions for growth and production of astaxanthin from Haematococcus pluvialis. The influence of different factors such as pH, temperature, agitation, aeration and lighting, CO2, promote cell growth, by giving an optimal environment to microalgae.

To determine the optimal nutritional conditions for cell growth the effect of different culture media (BBM, OHM, RM) in bioreactors of 500 ml with 350mL of medium and 1x104 cells/ml inoculum was evaluated in exponential phase, the culture conditions employed they were: pH. 6.7 to 7, 5% CO2, 16 hours photoperiod Light 8 dark, irradiance 70 μE / m2s; The results showed that the highest growth and cell reproduction was obtained in the middle RM with 7.5 x 105cells/ml on day 36, and increased production of astaxanthin was obtained in the middle RM with a concentration of 8.3 ug / ml in the treatment 4.

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DOI: http://dx.doi.org/10.22490/24629448.2073