Implementación y evaluación de dos métodos de conservación y generación de la base de datos del banco de cepas y genes del Instituto de Biotecnología de la Universidad Nacional de Colombia (IBUN)

Implementación y evaluación de dos métodos de conservación y generación de la base de datos del banco de cepas y genes del Instituto de Biotecnología de la Universidad Nacional de Colombia (IBUN)

76 strains of the familyEnterobacteriaceaeandPseudomonadaceaewere conserved by filter paper semistockmethod and 68 strains by Cryopreservation stock method in the bank of strains and genes of BiotechnologyInstitute of the Universidad Nacional de Colombia (IBUN). The effectiveness of methods was tested for theviability at 24 hours after preservation and was calculated the recovery percentage. Thus in conclusion, thepreservation in paper filter and cryopreservation are suitable semistock and Stock methods, respectively; because73.7% of familyEnterobacteriaceaemicroorganisms and 82.3% of family Pseudomonadaceae microorganisms,presented a high recovery percentage greater 50%. In addition, was implemented a strain bank data base madein software Access Æ, in Biotechnology Institute and Universidad Nacional de Colombia Web pagewww.ibun.unal.edu.co/instituto/cepario. It is available for general public.

1. Meza R, Monroy A, Mercado M, Poutou R, Rodríguez P, Pedroza A. Study of the stability in real time of cryopreserved strain banks. Universitas Scientiarum. 2004; 9(2):35-42
2. Espitia S, García Y. 1999. Caracterización y sistematización del banco de genes y cepas del Instituto de Biotecnología de la Universidad Nacional. Bacteriología y Laboratorio Clínico. Colombia, Universidad Colegio Mayor de Cundinamarca, Facultad de Ciencias de la Salud, 120 p.
3. Díaz M. 2004. Manual de procedimientos banco de cepas y genes. Bacteriología y Laboratorio Clínico. Colombia, Universidad Colegio Mayor de Cundinamarca, Facultad de Ciencias de la Salud, 50 p.
4. Kirsop B, Doyle A. 1991. Maintenance of microorganisms and cultured cells; A manual of laboratory methods. 2nd ed. London, Academic Press. 308p
5. Uruburu F. La sociedad española de microbiología y el aseguramiento de la calidad microbiológica. Actualidad SEM. 1997; 28: 12-13.
6. Frank P, Simione M. 1998. Cryopreservation Manual Nalge Nunc International. INTERNET: http://www.nalgenelabware.com/techdata/technical/cryo.pdf (Marzo,27, 2006)
7. MacFaddin J. 1985. Media for isolation-cultivationidentification maintenance of medical bacteria. USA. Williams & Wilkins. v. 1. 524p.
8. Gherna R. 1994. In Gerhardt, Murray, Wood and Krieg (ed.). Methods for general and molecular bacteriology. American society for microbiology. Washington, D.C. pp.278.292.
9. Sharp R. The preservation of genetically unstable microorganisms and the cryopreservation of fermentation seed cultures. Adv Biotechnol Processes. 1984; 3: 81.109
10. Silva J. TLA-1: A new plasmid-mediated extended-spectrum ß-lactamase from Escherichia coli. Antimicrob Agents Chemother. 2000; 44(4): 997.1003
11. Antheunisse J. Survival of microorganisms after drying and storage. Antonie van Leeuwenhoek. 1981; 47(6): 539-545
12. Nagai T. Evaluation of preservation techniques of microorganism resources in the maff genebank. Jarq. 2005; 39:19-27
13. Poutou R. 2002. Estudio de Estabilidad a Tiempo Real de Bancos de Cepas Criopreservados en Glicerol al 10% v/v. In: III Congreso Internacional de Microbiología Industrial. Bogotá, Colombia.
14. Fomin D, Alycheva I, Veselovskaya L, Tatchin S. Antibacterial properties of dimethylsulphoxide (in Russian), In: Khimioterapia infektsiy i lekarstvennoy ustoychivosti patogennych mikroorganizmov. Rusia, Moskva; 1983: pp.
15. 64
16. Hubàlek Z. Review: Protectans used in the cryopreservation of microorganisms. Cryobiology, 2003; 46 (3):205-229
17. Marcin A, Gyulai F, Arady J, Sorokov M. A Simple Technique for Cryopreservation of the rumen protozoa Entodinium simplex. Cryo-Letters. 1989; 10: 89.104
18. Daily W, Higgens C. Preservation and storage of microorganisms in the gas phase of liquid nitrogen.
19. Cryobiology. 1973;10: 364.367
20. Takahashi M. Effects of freezing and thawing and storage by freezing on the biological and morphological properties of strain K-9 of Klebsiella pneumoniae and its variants. Z Allg Mikrobiol. 1982; 22(9):649-60.
21. Centraalbureau voor Schimmelcultures. 2006. Kinds of microorganisms that may be deposited. INTERNET: http:// www.cbs.knaw.nl/patents/index.htm (Julio, 17, 2006)
22. -----------------------------------------------------------------------
23. DOI: http://dx.doi.org/10.22490/24629448.346