Detection of chlamydia trachomatis in men that have sex with men in Bogotá: a pilot study

Detección de Chlamydia trachomatis en hombres que tienen sexo con hombres en Bogotá: un estudio piloto

Published 2016-12-31

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Vol. 14 No. 26 (2016)
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Detection of chlamydia trachomatis in men that have sex with men in Bogotá: a pilot study. (2016). NOVA, 14(26), 17-27. https://doi.org/10.22490/24629448.1747
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Fredy Alberto Rodríguez Rojas
Universidad Colegio Mayor de Cundinamaca
    Paula Steffany Barreto Ordoñez
      Ruth Mélida Sánchez Mora
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        Objective. optimize a PCR technique to evaluate the presence of C. trachomatis in anorectal swabs from MSM. In Colombia there are reported each year more than 70,000 new cases of STIs, of which it is estimated that approximately 9.3% is urethritis among which are those caused by C. trachomatis. Methods. DNA extraction is one of the problems in the method of detecting C. trachomatis by PCR anorectal swab samples. Besides, the use of automated equipment arranged on the market is expensive and in many cases the samples are not available in the clinical laboratory routine. In this study it was performed PCR for detection of C. trachomatis protocol establishing the sampling and DNA extraction from anorectal swabs. Results. 27 samples were  processed corresponding HSH volunteers belonging to the Support group and study of Sexual Diversity (GAEDS) of the National University of Colombia. 5 samples positive for C. trachomatis associated with both symptomatic and asymptomatic men at high risk of acquiring infection because of their sexual practices were found.

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        1. World Health Organization. Baseline report on global sexually transmitted infection surveillance 2012. WHO Libr. Cat. 2012;52.
        2. Crickx E, Meignin V, Gérard L, Plantier-Colcher I. Rectal Lymphogranuloma Venereum in HIV-infected Patients Can Mimic Lymphoma. Journal of clinical gastroenterology, 2016; 50(5): 414-416.
        3. Ortiz OL, Sánchez Mora RM. Chlamydia trachomatis: avances y perspectivas. Nova, 2003; 1(1): 14-27.
        4. Coy G, Ostos OL, Pinzón J, Sánchez Mora, RM.
        5. Determinación de la asociación entre Chlamydia trachomatis y Neisseria gonorrhoeae, en muestras de orina mediante técnicas de biología molecular. Nova. 2004; 2(2): 28-32
        6. Paredes M, Gómez Y, Torres M, Fernández M & Tovar M. Prevalence of Chlamydia Trachomatis and Neisseria Gonorrhoeae in High School Students Between 14 and 19 Years-Old Using a Non Invasive Technique. Sexually Transmitted Infections. 2013; 8(1): A169-A169.
        7. Martínez T, Angélica M. Diagnóstico microbiológico de las infecciones de transmisión sexual (ITS): Parte 1. ITS no virales. Revista chilena de infectología. 2009; 26(6): 529-539.
        8. Carrera Páez LC, Pirajan Quintero ID, Urrea Suarez MC, Sánchez Mora RM, Gómez Jiménez M, Monroy Cano
        9. LA. Comparación del cultivo celular de HeLa y HEp2: Perspectivas de estudios con Chlamydia trachomatis. Nova.2015; 13(23):17-29.
        10. Martin DH, Nsuami M, Schachter J, Hook EW, Ferrero D, Quinn TC. Use of multiple nucleic acid amplification tests to define the infected-patient “gold standard” in clinical trials of new diagnostic tests for Chlamydia trachomatis infections. Journal of clinical microbiology. 2004; 42(10): 4749-4758.
        11. Jiang Y, Liu Y, Wang M, You C, Liu Q. Sensitivity of the Standard Chlamydia trachomatis Culture Method Is Improved After One Additional In Vitro Passage. Journal of clinical laboratory analysis. 2016.
        12. Waaij DJ, Dubbink JH, Peters RP, Ouburg S & Morré SA. Comparison of GMT presto assay and Roche cobas® 4800 CT/NG assay for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in dry swabs. Journal of microbiological methods. 2015; (118): 70-74.
        13. Villegas A, Tamayo L. Prevalence of sexually transmitted infections, and risk factor for sexual health of adolescents, Medellín, Colombia 2013. Iatreia. 2016; 29(1): 5-17.
        14. Tamayo L, López M, Villegas A, Arrubla M, Muñoz J. Determinantes de salud sexual e ITS en adolescentes rurales, escolarizados, Medellín Colombia 2011. Rev Salud Pública Medellín. 2011;(5):7-24.
        15. Paredes MC, Gómez YM, Torres AM, Fernández M, Tovar MB. Prevalence of infections by Chlamydia trachomatis and Neisseria gonorrhoeae among high school students in the Sabana Central area of Cundinamarca, Colombia. Biomédica. 2015; 35(3): 314-324.
        16. Occhionero M, Paniccia L, Pedersen D, Rossi G, Mazzucchini H, Entrocassi A et al. Prevalencia de la infección por Chlamydia trachomatis y factores de riesgo de infecciones transmisibles sexualmente en estudiantes universitarios. Revista argentina de microbiología. 2015;47(1): 9-16.
        17. Lengen C, Jäger S, Kistemann T. The knowledge, education and behaviour of young people with regard to Chlamydia trachomatis in Aarhus, Denmark and Bonn, Germany: do prevention concepts matter?. Soc Sci Med. 2010;70(11):1789–98.
        18. Vivancos R, AbubakarI, Phillips Howard P, Hunter PR. School-based sex education is associated with reduced risky sexual behaviour and sexually transmitted infections in young adults. Public Health.2013; 127(1): 53-57.
        19. Booth, AR, Norman P, Harris PR, Goyder E. Using the theory of planned behaviour and self‐identity to explain chlamydia testing intentions in young people living in deprived areas. British journal of health psychology. 2014; 19(1): 101-112.
        20. Peuchant O, Le Roy C, Herrmann B, Clerc M, Bébéar C, Barbeyrac B. MLVA subtyping of genovar E Chlamydia trachomatis individualizes the Swedish variant and anorectal isolates from men who have sex with men. PloS one. 2012; 7(2): 31-38.
        21. Williams D, Churchill D. Ulcerative proctitis in men who have sex with men: an emerging outbreak. Bmj. 2006; 332(7533): 99-100.
        22. Vaca Diana J. “ Identificacion Del Virus De Papiloma Humano Mediante Pcr-Rflp Y Posterior Genotipificacion En Muestras De Tejido Cervical Parafinado , Con Diagnóstico Histopatológico De Displasia Severa O Cáncer In Situ , Procedentes Del Hospital De Solca Núcleo Quito. Escuela Politécnica Del Ejército Departamento. 2012.
        23. H Smith F, Tenover CW. Detection of Chlamydia trachomatis. in Diagnostic molecular microbiology. Am Soc Microbiol. 1993;37.
        24. Markowicz M, Grilnberger E, Huber F, Leibl G, Abrahamian H, Gartner M, et al. Case report: lymphogranuloma venereum proctitis-from rapid screening to molecular confirmation of a masked sexually transmitted disease. El sevier. 2013; 76(4):516–7.
        25. Monteiro L, Bonnemaison D, Vekris A, Petry KG, Bonnet J, Vidal R, et al. Complex polysaccharides as PCR inhibitors in feces: Helicobacter pylori model. J Clin Microbiol. 1997; 35(4): 995-998.
        26. Roosendaal R, Walboomers JMM, Veltman, OR, Melgers I, Burger C, Bleker OP. Comparison of different primer sets for detection of Chlamydia trachomatis by the polymerase chain reaction. Journal of medical microbiology.1993; 38(6):426-433
        27. Ministerio de salud. Situación de las infecciones de transmisión sexual diferentes al VIH:2009-2011[internet] [consultado 2016 MAR 20]. Disponible en:https://www.minsalud.gov.co/ salud/Documents/observatorio_vih/documentos/monitoreo_ evaluacion/1_vigilancia_salud_publica/a_situacion_ epidimiologica/SITUACION%20DE%20LAS%20 INFECCIONES%20DE%20TRANSMISION1.pdf
        28. Cáceres CF, Konda K, Segura ER, Lyerla R. HSH casados, que hacen sexo con mujeres, transgéneros y trabajadores sexuales. Sex Transm Infect.2008; (84):49-56.
        29. Sanchez J, Lama JR, Kusunoki L, Manrique H, Goicochea P, Lucchetti et al. HIV-1, sexually transmitted infections, and sexual behavior trends among men who have sex with men in Lima, Peru. JAIDS Journal of Acquired Immune Deficiency Syndromes.2007; 4(5); 578-585.
        30. Páez LCC, Quintero IDP, Suarez MCU, Mora RMS, Jiménez MG, Cano LAM. Comparación del cultivo celular de HeLa y HEp-2: Perspectivas de estudios con Chlamydia trachomatis.
        31. Nova. 2015;13(23).
        32. ==========================================
        33. DOI: http://dx.doi.org/10.22490/24629448.1747
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