Blattella germanica (Blattodea: Blattellidae) as potential mechanical vector of health-care associated infections in a hospital in Villavicencio (Meta-Colombia)

Blattella germánica (Blattodea: Blattellidae) como potencial vector mecánico de infecciones asociadas a la atención en salud (IAAS) en un centro hospitalario de Villavicencio (Meta-Colombia)

Objective. Identify the species of cockroaches and bacteria associated with their exoskeleton in a hospital in Villavicencio (Meta, Colombia). Methods. The cockroaches were captured manually in kitchen, emergency room, intermediate intensive care unit, outpatient service and newborn nursery. The collected individuals were subjected to a primary isolation in BHI broth, then move on to solid media (blood and MacConkey). Identification and sensitivity testing was performed by automated method. Results. 24 nymphs and adults of Blattella germanica were collected. It was isolated and identified Klebsiella pneumoniae with intermediate susceptibility to meropenem (MIC 4) and resistance to cephalosporins (cefepime and cefuroxime) (> 16), Proteus vulgaris with cephalosporin resistance (Ceftriaxone, Cefuroxime) (MIC > 16), Enterobacter cloacae resistant to cephalosporins (cefoxitin, cefuroxime, ceftriaxone) (MIC > 16). Enterococcus faecium, Enterococcus rafinosus, Staphylococcus xylosus and Enterococcus faecalis. 80% of the isolated bacteria showed some degree of resistance to antibiotics. Results. These insects could play an important role in the transmission of health care associated infections (HCAI). Due to the presence of cockroaches in hospitals and the report of HCAI and bacterial resistance in several health centers in the Department of Meta, it is necessary to establish the relationship of these insects with these events.

1. Shukla P, Garg R, Dahiya A. Role of technology to combat nosocomial infections. Apollo Medicine. 2015; 338: 1-3.
2. Secretaria Distrital de Salud de Bogotá. Sistema de Vigilancia epidemiológica de infecciones intrahospitalarias.
3. Kassiri H, Kassiri A, Kazemi S. Investigaction on American cockroaches medically important bacteria in Khorramshahr hospital, Iran. Asian Pacific Journal of tropical Disease. 2014; 4(3): 201-203.
4. Rivera SM. Informe final infecciones asociadas a dispositivos, Colombia 2013. Informe evento. Bogotá: Instituto Nacional de Salud; 2013. Report No.: FOR-R02.4000-001.
5. Oliveira P, Souza S, Campos G, Da Silva D, Sousa D, Araujo S, et al. Isolation, pathogenicity and disinfection of Staphylococcus aureus carried by insects in two public hospitals in Vitoria da Conquista, Bahia, Brazil. Brazilian Journal Infection Diseases. 2014; 18(2): 129-136.
6. RENOVA. Informe microbiolópgico 2010-2012. 2012.
7. Instituto Nacional de Salud, Dirección de Redes en Salud Pública. Vigilancia de Resistencia Bacteriana a través de las bases de datos whonet. Año 2013.
8. Instituto Nacional de Salud. Vigilancia de Resistencia bacteriana Whonet - I semestre 2015. 2015 Diciembre.
9. Olaya LA, Chacón de Ulloa P, Payán A. Hormigas (Hymenoptera: Formicidae) en centros hospitalarios del Valle del Cauca como vectores patógenos nosocomiales. Revista Colombiana de Entomología. 2005; 31(2): 183-187.
10. Trasmonte A, García Y, Humbria L, García de Humbría G, Cazorla D. Aislamiento de enterobacterias en la mosca común (Musca domestica) en Coro, estado de Falcón, Venezuela. Boletín de Malariología y Salud Ambiental. 2009; XLIX(2): 275-281.
11. Jalil N, Amir K, Hasan MK, Mahdi M, Monireh M, Atefeh B. Cockroaches bacterial infections in wards of hospitals, Hamedan city, west of Iran. Asian Pacific Journal of Tropical Disease. 2012; 381-384.
12. Ghasemi_Dehkordi P, Doosti A, Doosti E, Noshadi E, Arshi A. Antimicrobial susceptibility patterns of Escherichia coli isolates from cockroaches in southwestern Iran. Bulgarian Journal of Veterinary Medicine. 2015; 1-9.
13. Tetteh-Quarcoo P, Donkor E, Attah S, Duedu K, Afutu E, Boamah I, et al. Microbial carriage of cockroaches at a tertiary care hospital in Ghana. Environmental Health Insights. 2013; 7: 59-66.
14. Tilahun B, Worku B, Tachbele E, Terefe S, Kloos H, Legesse W. High load of multu-drug resistant nosocomial neonatal pathogens carried by cockroach in a neonatal intensive care unit at Tikur Anbessa specialized hospital, Addis Ababa, Ethiopia. Antimicrobial Resistance and Infection Control. 2012; 1(12): 1-7.
15. Menasria T, Tine S, Hamza S, Mahcene D, Moussa F, Benammar L, et al. A survey of the possible role of german cockroaches as a source for bacterial pathogens. Journal of Advanced Sciences & Applied Engineering. 2014; 1(1): 67-70.
16. Adeleke MA, Akatah HA, Hassan AO, Sam-Wobo SO, Famodimu TM, Olatunde GO, et al. Implication of cockroaches as vectors of gastrointestinal parasites in parts os osogobo, southwestern Nigeria. Munis Entomology & Zoology. 2012; 7(2): 1106-1110.
17. Handool H, Al.Aridhi H. Antibiotic resistant bacteria associated with the cockroach Periplaneta americana in Al-Diwaniya city /Iraq. International Journal of Advnaced Research. 2014; 2(2): 709-7014.
18. Salehzadeh A, Tavacol P, Mahjub H. Bacterial, fungal and parasitic contamination of cockaroaches in public hospitals of Hamadan, Iran. Journal of Vector Borne Disease. 2007; 44: 105-110.
19. Menasria T, Moussa F, El-Hamza S, Tine S, Megri R, Chenchouni H. Bacterial load of german cockroach (Blattella germanica) found in hospital environment. Pathogens and Global Health. 2014; 108(3): 141-147.
20. Piazuelo MM, Jaramillo I, Gonzalez R. Resistencia a deltametrina de cepas de Blattella germanica (Dyctioptera: Blattellidae) en la ciudad de Cali, Colombia. Revista Cubana de Medicina Tropical. 2009; 61(3): 213-219.
21. Xue FU, Lefu YE, Feng GE. Habitat influences on diversity of bacteria found on german cockroach in Beijing. Journal of Environmentla Sciences. 2009; 21: 249-254.
22. Jaramillo I, Cardenas H, Gonzalez R, Rosero C. Genetic variability of five Periplaneta americana l. (Dyctiopteria: Blattellidae) populations in southwestern Colombia using the AFLP molecular marker technique. Neotropical Entomology. 2010; 39(3): 371-378.
23. Akinjogunla OJ, Odeyemi AT, Udoinyang EP. Cockroaches (Periplaneta americana and blattella germanica): reservoirs of multi drug resistant (MDR) bacteria in Uyo, Akwa Ibom State. Scientific Journal of Biological Sciences. 2012; 1(2): 19-30.
24. Kassiri H, Kazemi S. Cockroaches (Periplaneta americana (L.), Dyctioptera; Blattellidae) as carriers of bacterial pathogens, Khorramshahr County, Iran. Jundishapur Journal of Microbiology. 2012; 5(1): 320-322.
25. Cercenado E, Saavedra J. El antibiograma. Interpretación del antibiograma, conceptos generales. Annales de Pediatria Continuada. 2009; 7(4): 214-217.
26. Instituto Nacional de Salud. Resistencia bacteriana a los antimicrobianos en el ámbito hospitalario. protocolo de Vigilancia en Salud Pública. Bogotá: Instituto Nacional de Salud; 2014. Report No.: PRO-R02-043.
27. Galvez, Z. Y. A. and V. E. M. Burbano (2015). “Bacillus: género bacteriano que demuestra ser un importante solubilizador de fosfato.” NOVA Publicación en Ciencias Biomédicas.
28. Ramírez, L. C. C., et al. (2014). “Determinación de la presencia de bacterias patógenas para el humano en aguas de riego en la cuenca alta de la sabana de Bogotá; DC Colombia.” Nova 12(22).
29. Campuzano, S., et al. (2015). “Determinación de la calidad microbiológica y sanitaria de alimentos preparados vendidos en la vía pública de la ciudad de Bogotá DC.” Nova.
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31. DOI: http://dx.doi.org/10.22490/24629448.1723