Influence of a-Factor over the expression of hrIDS inPichia pastoris: Literature review andcomputational analysis.

Influencia del a-Factor sobre la expresión de IDShr en Pichia pastoris: Revisión sistemática de literatura y análisis computacional

In searching of alternatives to get the efficient secretions of the recombinant enzymeIduronate Sulfatase (IDSh) inPichia pastoris,we made a systematic review of the literature so that we couldcompile information to relate the expression level of human heterologous proteins, with secretion signal, and the characteristics of the molecules. 349 publications were found of which only 7 (2%) reported the expression ofproteins that met find inclusion criteria. With the information obtained in these articles, we made a hypothesistest using the data compiled as samples and a qualitative analysis of the information. It was found that replacementof native secretion signal bya-Factor as leader peptide increases the level of expression of human heterologous proteins inP. pastoris(p=0.053). Moreover, the elimination of native signal peptide in the protein cDNA is 125 indispensable for the function of a-Factor in the secretion of recombinant proteins and for to increase the expression level. In the construct, GS115/pPIC9-IDS were simultaneously founded two sequences in the signal peptide, the native and thea-Factor. However, the activity found ~30 mmol/h mg of protein raises the questionof a possible conflict between the two recognition sites. This may be the cause given the similarity in the hidrophobicity of those two sites.

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60. DOI: http://dx.doi.org/10.22490/24629448.339