Tamizaje serológico y con PCR para determinar la prevalencia de Chlamydia trachomatis en pacientes con vaginosis y vaginitis inespecífica que asisten a hospitales de la Secretaría de Salud de Bogotá

Tamizaje serológico y con PCR para determinar la prevalencia de Chlamydia trachomatis en pacientes con vaginosis y vaginitis inespecífica que asisten a hospitales de la Secretaría de Salud de Bogotá

Published 2005-07-01

Open | Download
PDF (Spanish) FLIP HTML (Spanish)
Issue
Vol. 3 No. 3 (2005)
Section
Artículo Original
How to Cite
Tamizaje serológico y con PCR para determinar la prevalencia de Chlamydia trachomatis en pacientes con vaginosis y vaginitis inespecífica que asisten a hospitales de la Secretaría de Salud de Bogotá. (2005). NOVA, 3(3). https://doi.org/10.22490/24629448.20
doi

https://doi.org/10.22490/24629448.20
Dimensions
PlumX
license

Licencia Creative Commons

NOVA by http://www.unicolmayor.edu.co/publicaciones/index.php/nova is distributed under a license creative commons non comertial-atribution-withoutderive 4.0 international.

Furthermore, the authors keep their property intellectual rights over the articles.

 

Maria Teresa Herrera Mendoza
Universidad Colegio Mayor de Cundinamarca
    Ruth Melida Sanchez
    Universidad Colegio Mayor de Cundinamarca
      Ariel Iván Ruiz Parra
      Hospital Materno Infantil
        Olga Lucia Ostos
        Universidad Colegio Mayor de Cundinamarca Universidad Antonio Nariño
          Show authors biography
          Serologic and PCR screening to determine the prevalence of chlamydia trachomatis in patients with vaginitis and inespecific vaginitis in two public hospitals in Bogota: Chlamydia trachomatis is one of the most important agents in the aetiology of sexually transmitted diseases (STD). In women CT causes cervicitis,  urethritis and pelvic inflammatory disease. The objectives of this investigation were to determine the prevalence of chlamydial infection and the concordance of the diagnostic tests in patients with vaginitis and inespecific vaginitis who assist to two public hospitals of Bogota (Hospital Pablo VI de Bosa and Hospital del Sur), during June and July 2004. IgG, IgM and IgA in serum and PCR in urine samples were practiced in 180 patients. C. trachomatis antibodies detection was done by ELISA (VIRCEL) and DNA detection by AMPLICOR CT PCR (ROCHE) test. The prevalence of chlamydial infection was 31% (56 cases). The median age of women with recent or active infection was 23 years. 37 patients (66%) with positive results in at least one marker had active infection. DNA was not detected in urine samples in 24 patients (42.8 %) with active infection diagnosed by serologic criteria. PCR was positive in urine samples of 14 women (25 % of the patients with positive serologic tests). A prevalence of 31% in symptomatic women indicates a mayor public health problem. It is necessary to implement a screening programme to detect the cases, as well as, to find the sexual contacts in order to reduce the burden of chlamydial infection. A urine sample mixed with a cervical simple will be useful for the simultaneous diagnosis of cervicitis and urethritis.     

          Article visits 274 | PDF visits 273

          Downloads

          Download data is not yet available.
          1. Basuado JA, Cuto C, De Torres R. Microbiología Biomédica. Ed. Atlante. Buenos Aires, Argentina 1996; 38:420-426.
          2. Bauwens JE, Clark AM, Stamm WE, Diagnosis of Chlamydia trachomatis endocervical infections by a commercial polymerase chain reaction assay. J. Clin. Microbiology.1993; 31:3023-3027.
          3. Black C. Current Methods of Laboratory Diagnosis of Chlamydia trachomatis Infections. Clinical Microbiology Reviews. 1997; (18):160 . 184.
          4. Centers for Disease Control and Prevention. Guidelines for treatment of sexually transmitted diseases. MMWR. 1998; 47: 1-116.
          5. Chan EL, Brandt K, Stoneham B, Antonishyn N, Horsman G, Archivos de Patología y la Medicina del Laboratorio. Northfield. 2000; 124 (6): 840p.
          6. Chavez M, Vargas J, Pueyo I, Valverde A, Serrano M, Claro R, Manzuelos ME. Incidencia de la infección genitourinaria por Chlamydia trachomatis en un centro de ETS, estimada mediante detección directa de antígeno. Enferm. Infec. 2000; 18:392-395.
          7. Dean D, Suchland R, Stam W. Evidence for Long . term cervical persistence of Chlamydia trachomatis by omp I genotyping. J Infect Dis. 2000; 182:909- 916.
          8. Eng T, Butler W. The Hidden Epidemic: confronting sexually transmited diseases. Washington, D.C. National Academy Press.2003.
          9. Fauci AS, Braunwald E, Isselbucher K. Principios de medicina Interna. McGrawHill. 1998; 181:1209-1216
          10. KFF- Kaiser Family Foundation. Sexually Transmited Diseases in America. How many Cases and what Cost ? Mela Park, CA. Kaiser Family Foundation and American Social Health Association. 1998.
          11. Labay E, Henry S, Bennet P, Massip P, Chabanon G. Direct Diagnosis of Chlamydia trachomatis Genital Infections: Culture or PCR? Pathol Biol Paris. 1998; 46(10):813-818.
          12. Madico G, Quinn TC, Boman J, Gaydos CA. Touch down enzyme time release- PCR for detection and identification of Chlamydia trachomatis, Chlamydia pnemoniae and Chlamydia psittacci using the 16S and 16 S 23 C Spacer rRNA genes. J Clin Microbiol. 2000; 38:1085 -1093.
          13. Morris R, Legault I, and Baker C. Prevalence of isolated uretral asymptomatic Chlamydia trachomatis infection in the absence of cervical infection of in carcelated adolescent girls. Sex Transm Dis. 1993;20:198-200.
          14. Pimienta J, Frento K, Tendencias Recientes de las Infecciones por Chlamydia trachomatis en el Reino Unido y Perspectivas de un programa de Detección Nacional. Public. Health Laboratory Service Comunicable Disease Surveillance Centre. Londres Inglaterra. Comm. Dis. Public Health.2003.
          15. Sellor JWI, Mahony B, Jang D, et al. Comparison of cervical, urethral, and urine specimens for the detection of Chlamydia trachomatis in women. J Infect Dis. 1998;164:205-208.
          16. Servass AM, Spaargaren J, Schmid G, Pena AS, et. al. Persistence of Chlamydia trachomatis infections: bacterium and hort bast? /.Reply. J Infec Dis. 2001; Vol:184:12:1631.
          17. Watson E, Templeton A, Paavonen J, Mardh P, Stary A, Pedeson BS. The accuracy and efficacy of screening test for Chlamydia trachomatis: a systematic. Review. J Med Microbiol. 2002; (51): 1021 . 1031.
          18. -------------------------------------------------------------------------------
          19. DOI: http://dx.doi.org/10.22490/24629448.20
          Tutorials

          Autors:

          How send article?

          Evaluator pair

          How to evaluate an article?

          Information

          Keywords

          Google Scholar profile

          Is part of
           

          Current Issue

          Sistema OJS 3.4.0.5 - Metabiblioteca |