Determination of genes, 16 S A DNr, p ol A, and Tp N 4 7, in the detection of Treponema pallidum subsp. pallidum in the diagnosis of congenital syphilis

Determinación de los genes, 16S ADNr, polA, y TpN47, en la detección de Treponema pallidum subsp. pallidum para el diagnóstico de sífilis congénita

Published 2015-12-30

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Vol. 13 No. 24 (2015)
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Artículo Original Producto de Investigación
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Pinilla B., G., Chavarro, B., Moreno, N., Navarrete, J., & Muñoz, L. (2015). Determination of genes, 16 S A DNr, p ol A, and Tp N 4 7, in the detection of Treponema pallidum subsp. pallidum in the diagnosis of congenital syphilis. NOVA, 13(24), 17-26. https://doi.org/10.22490/24629448.1713
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https://doi.org/10.22490/24629448.1713
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Gladys Pinilla B.
Universidad Colegio Mayor de Cundinamarca
    Bibiana Chavarro
    Universidad Colegio Mayor de Cundinamarca
      Natalia Moreno
      Universidad Colegio Mayor de Cundinamarca
        Jeannette Navarrete
        Universidad Colegio Mayor de Cundinamarca
          Liliana Muñoz
          Universidad Colegio Mayor de Cundinamarca
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            Objective. To compare the behavior of the three target genes (16SrDNA, polA, and Tp N47) for the detection of T. pallidum subsp. Pallidum. Methods. Molecular techniques such as polymerase chain reaction were used on samples of an umbilical cord. Molecular targets of the microorganism were amplified by means of conventional PCR, nested PCR and real-time PCR. Results. Similar results for the three genes were obtained by conventional PCR; but in the case of nested PCR and real-time PCR, the gen TpN47 exhibited higher sensitivity in comparison to the genes polA and 16S rDNA. In conclusion, the genTp N47 can be used as a molecular target for the prompt diagnosis of congenital syphilis through nested PCR and real-time PCR due to its high sensitivity and specificity shown in this study.

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            37. DOI: http://dx.doi.org/10.22490/24629448.1713
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