Comparison of cell culture HeLa and HEp-2: Studies prospects Chlamydia trachomatis

Comparación del cultivo celular de HeLa y HEp-2: Perspectivas de estudios con Chlamydia trachomatis

Published 2015-06-30

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Vol. 13 No. 23 (2015)
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Artículo Original Producto de Investigación
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Carrera Páez, L. C., Pirajan Quintero, I. D., Urrea Suarez, M. C., Sanchez Mora, R. M., Gómez Jiménez, M., & Monroy Cano, L. A. (2015). Comparison of cell culture HeLa and HEp-2: Studies prospects Chlamydia trachomatis. NOVA, 13(23). https://doi.org/10.22490/24629448.1371
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https://doi.org/10.22490/24629448.1371
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Laura Camila Carrera Páez
Universidad Colegio Mayor de Cundinamarca
    Ingrid Daniela Pirajan Quintero
    Universidad Colegio Mayor de Cundinamarca
      María Camila Urrea Suarez
      Universidad Colegio Mayor de Cundinamarca
        Ruth Melida Sanchez Mora
        Universidad Colegio Mayor de Cundinamarca
          Martha Gómez Jiménez
          Universidad Colegio Mayor de Cundinamarca
            Luz Adriana Monroy Cano
            Universidad Colegio Mayor de Cundinamarca
              Show authors biography
              Objective. The goal of this study was to standardize the cultivation of HeLa cells under different conditions, to be used in Chlamydia trachomatis serovar L2 infection’s protocols. Materials and Methods. This study was conducted in four phases that are: 1.Cell Viabilidad by trypan blue technique and his subsequent remark, 2. Standardization HeLa cell culture, 3. Giemsa, 4. Cultivation of Hep-2 cells. Results. As a result of standardization it is determined that the HeLa cell line should be cultured in DMEM, 0.1% L-glutamine, 10% FBS.It was determined that the Giemsa perform better over time of 40 minutes that a clear definition of nucleus and cytoplasm is evident. Comparing against both cell lines HeLa was obtained that the line from day growing well and quickly reaches the expected confluence, the opposed line HEp-2 has a slower growth but achieving the desired confluence the last day.

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              34. DOI: http://dx.doi.org/10.22490/24629448.1371
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