Cultivation of the cell line hep-2: dubbing of population and coloring with Giemsa

Cultivo de la línea celular HEp-2: doblaje poblacional y coloración con Giemsa. Perspectivas para el estudio de la infección con Chlamydia trachomatis

Published 2013-12-31

Open | Download
PDF (Spanish) FLIP HTML (Spanish)
How to Cite
Cultivation of the cell line hep-2: dubbing of population and coloring with Giemsa. (2013). NOVA, 11(20). https://doi.org/10.22490/24629448.1836
doi

https://doi.org/10.22490/24629448.1836
Dimensions
PlumX
license

Licencia Creative Commons

NOVA by http://www.unicolmayor.edu.co/publicaciones/index.php/nova is distributed under a license creative commons non comertial-atribution-withoutderive 4.0 international.

Furthermore, the authors keep their property intellectual rights over the articles.

 

AP Jutinico Shubach
Universidad Colegio Mayor de Cundinamarca.
    J Malagón Garzón.
    Universidad Colegio Mayor de Cundinamarca.
      J N Manrique Chacón
      Universidad Colegio Mayor de Cundinamarca.
        M Gómez
        Universidad Colegio Mayor de Cundinamarca.
          RM Sánchez Mora
          Universidad Colegio Mayor de Cundinamarca.
            Show authors biography
            Cell cultures have become essential tools for basic research. They are applied in immunology, virology, molecular biology, genetic engineering and pharmacology, among other areas. They are also used in pharmaceutical industrial processes, in techniques of clinical diagnostic, and to study tissue transplantation. In bacteriology, these crops allow us to confirm an infection, assess the efficiency of antimicrobials, carry out studies of infectivity, investigate on new species, obtaining a large number of microorganisms non-arable to optimize techniques, and to examine the relationship between the host cell and intracellular microorganisms (bacteria, viruses, and parasites). The HEp-2 cell line (Human epidermoid cancer cells) is used in studies of infection with different bacteria, including Chlamydia trachomatis (CT), in order to determine the mechanisms by which the pathogen survives in the host cell. It is also used to observe the action of antimicrobial peptides and extracts to combat the infection caused by the bacterium. For this study, growth curves of the HEp-2 cell line were carried out with DMEM-F12 and MEM media. In addition, the staining with Giemsa was standardized, and the population dubbing was calculated with differentinocula for assessing the development of the cultured cell line and select the optimal conditions for future tests of infection with intracellular parasites, in particular with CT serovar L2.

            Article visits 179 | PDF visits 134

            Downloads

            Download data is not yet available.
            1. Draganov M, Fransazov S, Draganov D, Murdjeva M, Popov N. Two new serum free and protein free cell strains, derived from HEp-2 cell line: cultural conditions and proliferation activity. Journal of Culture Collections 2009;6(1):112-21.
            2. Hotez PJ, Bottazzi ME, Franco-Paredes C, Ault SK, Periago MR. The neglected tropical diseases of Latin America and the Caribbean: a review of disease burden and distribution and a roadmap for control and elimination. PLoS Negl Trop Dis 2008;2(9):e300.
            3. Cervantes E. Infecciones causadas por Chlamydia trachomatis. Rev Fac Med UNAM 2009;52(1):18-22.
            4. Kubo T, Ishida K, Matsuo J, Nakamura S, Hayashi Y, Sakai H, et al. Chlamydia trachomatis serovar L2 infection model using human lymphoid Jurkat cells. Microb Pathog 2012 Jul;53(1):1-11.
            5. López E. Estudio de las características del ciclo de desarrollo de Chlamydia trachomatis relacionadas a la presencia del plásmido 7.5 Kb Instituto Politecnico Nacional; 2009.
            6. Kameswaran TR, Ramanibai R. Indirubin-3-monooxime induced cell cycle arrest and apoptosis in Hep-2 human laryngea carcinoma cells. Biomed Pharmacother 2009 Feb;63(2):146-54.
            7. Wiedeman JA, Kaul R, Heuer LS, Thao NN, Pinkerton KE, Wenman WM. Tobacco smoke induces persistent infection of Chlamydophila pneumoniae in HEp-2 cells. Microb Pathog 2004 Sep;37(3):141-8.
            8. Hao H, Aixia Y, Dan L, Lei F, Nancai Y, Wen S. Baicalin suppresses expression of Chlamydia protease-like activity factor in Hep-2 cells infected by Chlamydia trachomatis. Fitoterapia 2009 Oct;80(7):448-52.
            9. Life Technologies Corporation. PrFont34Bin0BinSub0Frac-
            10. Def1Margin0Margin0Jc1Indent1440Lim0Lim11.http://www.invitrogen.com/site/us/en/home/support/Product-Technical-Resources/media_formulation.1.html. 20-2-2013. Ref Type: Online Source
            11. Sigma. ttp://www.sigmaaldrich.com/etc/medialib/docs/Sigma/ Formulation/m4655for.Par.0001.File.tmp/m4655for.pdf. 20-2- 2013. Ref Type: Online Source.
            12. Invitrogen.http://www.jbc.org/content/suppl/2009/02/26/ M900773200.DC1/Schmidetal_Supplements.pdf. 20-2-2013. Ref Type: Online Source.
            13. Skinner MC, Kiselev AO, Isaacs CE, Mietzner TA, Montelaro RC, Lampe MF. Evaluation of WLBU2 peptide and 3-O-octylsn-glycerol lipid as active ingredients for a topical microbicide formulation targeting Chlamydia trachomatis. Antimicrob Agents Chemother 2010 Feb;54(2):627-36.
            14. Lazarev VN, Polina NF, Shkarupeta MM, Kostrjukova ES, Vassilevski AA, Kozlov SA, et al. Spider venom peptides for gene therapy of Chlamydia infection. Antimicrob Agents Chemother 2011; Nov;55(11):5367-9.
            15. Life Technologies Corporation. HYPERLINK "http://www. invitrogen.com/site/us/en" http://www.invitrogen.com/site/ us/en home/support/Product-Technical-Resources/media_ formulation.1.html. 20-2-2013. Ref Type: Online Source.
            16. Invitrogen. HYPERLINK "http://www.jbc.org/content/ suppl/2009/02/26" http://www.jbc.org/content/ suppl/2009/02/26 M900773200. / Schmid etal _Supplements. pdf. 20-2-2013. Ref Type: Online Source.
            17. López E. Estudio de las características del ciclo de desarrollo de Chlamydia trachomatis relacionadas a la presencia del plásmido 7.5 Kb Instituto Politecnico Nacional; 2009.
            18. Sasiaín J, Instituto Nacional de Salud (Colombia). Reseña de "cien años del colorante Giemsa". Biomédica 2003 Mar;23:5-18.
            19. Lazarev VN, Polina NF, Shkarupeta MM, Kostrjukova ES, Vassilevski AA, Kozlov SA, et al. Spider venom peptides for gene therapy of Chlamydia infection. Antimicrob Agents Chemother 2011 Nov;55(11):5367-9.
            20. Yasin B, Pang M, Lehrer RI, Wagar EA. Activity of Novispirin G-10, a novel antimicrobial peptide against Chlamydia trachomatis and vaginosis-associated bacteria. Exp Mol Pathol 2003; Apr;74(2):190-5
            21. -----------------------------------------------------------------------------------
            22. DOI: http://dx.doi.org/10.22490/24629448.1836
            Tutorials

            Autors:

            How send article?

            Evaluator pair

            How to evaluate an article?

            Information

            Keywords

            Google Scholar profile

            Is part of
             

            Current Issue

            Sistema OJS 3.4.0.5 - Metabiblioteca |