Effect of Mercurius-Heel®s on the cytotoxicity of human gingival fibroblasts in an in vitro model

Efecto del Mercurius-Heel®s sobre la citotoxicidad de fibroblastos gingivales humanos en un modelo in vitro

Published 2013-12-31

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Vol. 11 No. 20 (2013)
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Artículo Original Producto de Investigación
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Effect of Mercurius-Heel®s on the cytotoxicity of human gingival fibroblasts in an in vitro model. (2013). NOVA, 11(20). https://doi.org/10.22490/24629448.1027
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https://doi.org/10.22490/24629448.1027
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Gabriela Rueda
Pontificia Universidad Javeriana.
    Dabeiba Adriana García
    Pontificia Universidad Javeriana.
      Liliana Catherine Patiño
      Pontificia Universidad Javeriana.
        Angélica Cagueñas
        Pontificia Universidad Javeriana.
          Mariana García
          Pontificia Universidad Javeriana.
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            Periodontal disease is a pathology of infectious origin, characterized because it causes destructive consequences to the supporting tissue of the tooth, its treatment is aimed at the destruction of the etiologic agents and to the periodontal regeneration. An alternative is the use of homeopathic agents because they are natural and are managed to very low concentrations, one of them is the Mercurius Heel ® S as an adjunctive therapy in infectious diseases. In this work, we present the results of an investigation whose objective was to evaluate the effect of Mercurius Heel® S on the viability of human gingival fibroblasts, which were undergoing treatment with Mercurius Heel® S for 15 minutes and two hours to concentrations from 300mg to 0.00006mg. After these times, the treatment was removed and the cells were maintained for 24, 48, and 72 more hours. It was followed by a colorimetric assay for cell viability and proliferation called MTS of Promega ®.   Human gingival fibroblasts treated with Mercurius Heel® S showed an increase in cell proliferation compared with the untreated cells. Low medication concentrations of 0.0001 mg and 0.00006 mg showed a greater proliferation showed greater statistically differences. Finally, the effect of Mercurius Heel® was maintained for the first 48 hours. Considering the above, the Mercurius Heel® did not provide any cytotoxic effect on human gingival fibroblasts; on the contrary, the cells proliferated, suggesting its usefulness as supplementary treatment for periodontal disease.

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            22. DOI: http://dx.doi.org/10.22490/24629448.1027
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