Mycoses for Pythium insidiosum. First case with definitive diagnosis in Colombia

Micosis por Pythium Insidiosum primer caso con diagnóstico definitivo en Colombia

Pythium insidiosum is a globally distributed fungus that causes a fungal infection called Pythiosis is considered an emerging disease in humans and animals. A definitive diagnosis involves clinical, epidemiological, histopathological and molecular correlations. There are no reports of Pythiosis diagnosis involving all these correlations in Colombia. In this study were confirmed Phytiosis in two horses in rural area of Puerto Boyacá (Boyacá, Colombia) presenting single,  itching, ulcerated, granulomatous lesions on their limbs with serosanguineous suppuration. Biopsies were analyzed pathologically and histopathologically. Polymerase Chain Reaction (PCR) was carried out from DNA extracted of paraffin blocks. This study report the first definitive diagnosis of phytiosis in Colombia based on the correlation between bio-geography area, the pathological and histopathological findings which included patterns like Splendore-Hoeplii and hyphae with rare septae (2.5 to 6.2 microns diameters of branching angled) along with sequenced DNA that showed 100% homology with the Pythium insidiosum sequence at the GenBank.

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