Actividades hidrolíticas y caracterización isoenzimática de poblaciones microbianas aisladas del patrimonio documental del Archivo General de Colombia

Luz Stella Villalba; José Fernando Mikán; Jimena Sánchez

doi:10.22490/24629448.7

Hydrolytic activity and isoenzymatic characterization of microbial population Isolated ofdocumental Heritage of General Archive of Colombia (AGN)

Actividades hidrolíticas y caracterización isoenzimática de poblaciones microbianas aisladas del patrimonio documental del Archivo General de Colombia

Published 2004-12-31

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Hydrolytic activity and isoenzymatic characterization of microbial population Isolated ofdocumental Heritage of General Archive of Colombia (AGN). (2004). NOVA, 2(2). https://doi.org/10.22490/24629448.7

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https://doi.org/10.22490/24629448.7

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NOVA by http://www.unicolmayor.edu.co/publicaciones/index.php/nova is distributed under a license creative commons non comertial-atribution-withoutderive 4.0 international.

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Luz Stella Villalba

Laboratorio de análisis y control, Archivo de Bogotá

José Fernando Mikán

Facultad de Medicina, Universidad Militar Nueva Granada

Jimena Sánchez

Departamento de biología, Universidad Nacional de Colombia

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Biodeterioration

Microbial population

Enzymatic hydrolytic

Isoenzyme

This work shows an isolation and evaluation model of microorganisms deterioration agents of documental heritage. 28 Isolations were recovered and identified as conservation with biodeterioration as in deposit No. 15 atmosphere of AGN (Bogotá-Colombia). Of this group, 16 microbial isolations showed hydrolytic capability on vegetable fibers when they were cultivated in with cellular walls at 1% as the unique carbon source. In the recovered microbial populations were evaluated the capability to hydrolyze cellulose, xylane, almidon and protein. Taking into account degradation haloes and the number of hydrolyzed substrates were selected four isolations: two of Penicillium sp., one of Bacillus sp. and one of Actinopolyspora sp.. Polymerase activities and accessories were quantified on them and it was determined Isoenzymatic profile to cellulase and xilanase. The results suggest: filamentous fungi and Actinomycete are more efficient degradation complex polymer (cellulose), probably the bacterial population acts as a secondary settler and Isoenzymatic profile permits discard saprophyte microorganisms, specialized in degrading cellulolytic support.

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