Evaluación de características morfológicas e inmunofenotipo de células madre mesenquimales en cultivo obtenidas a partir de sangre de cordón umbilical y médula ósea.
Evaluación de características morfológicas e inmunofenotipo de células madre mesenquimales en cultivo obtenidas a partir de sangre de cordón umbilical y médula ósea.
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Páez Guerrero, D., Arévalo Romero, J., & Rodríguez Pardo, V. M. (2007). Evaluación de características morfológicas e inmunofenotipo de células madre mesenquimales en cultivo obtenidas a partir de sangre de cordón umbilical y médula ósea. NOVA, 5(8). https://doi.org/10.22490/24629448.380
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Bone marrow and umbilical cord blood are sources of mesenchymal stem cells. They are important for tissue regeneration and immunomodulation. The object of this work was to isolate and culture mesenchymal stem cells from bone marrow and umbilical cord blood cells. Bone marrow samples were obtained from patients at the Orthopedic Service of the San Ignacio Hospital and cord blood samples were obtained from women with normal pregnancy at the Gineco-Obstetrics Service of the Occidente de Kennedy Hospital in Bogotá, Colombia. We determined cellular rate generation, morphologic characteristics and antigens expression for cytometry flow.
Efficiency isolation was 30% for mesenchymal stem cells from umbilical cord blood and 100% for bone marrow, with cellular rate generation between 20 and 50 minutes and 16 and 39 minutes respectively. Morphologic differences were determined with microscopy and Wright coloration. During primary culture, 3.54% of CD34+/CD45+ was observed, but after 10 days, they were at 0.19% (CD34+/CD45+). Isolation of mesenchymal stem cells from bone marrow is efficient. There are differences between mesenchymal stem cells from umbilical cord blood and bone marrow. CD105 antigen is an important marker for mesenchymal cells lineage.
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- Körbling M, Estrov Z. Adult stem cells for tissue repair – a new therapeutic concept?. N Engl J Med. 2003;349:570-582.
- Rodríguez V. Células Madre:Conceptos Generales y Perspectivas de Investigación. Universitas Scientiarum. 2005;10:5-14.
- Szilvassy S. The biology of hematopoietic stem cells. Arch Med Res. 2003; 34: 446-60.
- Kang X, Zang W, Bao L, Li D, Xu X, Yu X. Differentiating characterization of human umbilical cord blood derived mesenchymal stem cells in vitro. Cell Biol Int. 2006;30:569-575.
- Beyer N, Da Silva L. Mesenchymal Stem Cells: Isolation in vitro expansion and characterization. Handb Exp Pharmacol. 2006;174:249-282.
- Gregory C, Prockop D, Spees J. Non-hematopoietic bone marrow stem cells: Molecular control of expansion and differentiation. Exp Cell Res. 2005; 306:330-335.
- Bianco P, Riminucci M, Gronthos S, Robey P. Bone Marrow Stromal Cells: Nature, biology, and potencial applications. Stem Cells. 2001; 19: 180-92.
- Rasmusson I. Immune modulation by mesenchymal stem cells. Exp Cell Res. 2006;312:2169-2179.
- Deans RJ, Moseley AB. Mesenchymal Stem Cells: Biology and potential clinical uses. Exp Hematol. 2000;28:875-884.
- Lakshmipathy U, Verfaille C. Stem Cell Plasticity. Blood Rev. 2005;19:29-38.
- Wexler S, Donaldson C, Denning P, Rice C, Bradley B, Hows J. Adult bone marrow is a rich source of human mesenchymal «stem» cells but umbilical cord and mobilized adult blood are not. Br J Haematol. 2003;121:368-374.
- Baksh D, Song L, Tuan RS. Adult mesenchymal stem cells: characterization, differentiation, and application in cell and gene therapy. J Cell Mol Med. 2004;8:301-316.
- Wagner W, Wein F, Seckinger A, Frankhauser M, Wirkner U et al. Comparative characteristics of mesenchymal stem cells from human bone marrow, adipose tissue, and umbilical cord blood. Exp Hematol. 2005; 33:1402-1416.
- Kern S, Eichler H, Stoeve J, Klüter H, Bieback K. Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue. Stem Cells. 2006;24:1294-1301.
- Bieback K, Kern S, Klüter H, Eichler H. Critical parameters for the isolation of mesenchymal stem cells from umbilical cord blood. Stem Cells. 2004; 22:625-634.
- Sabatini F, Petecchia I, Tavian M, Jodon V, Rossi G, Brouty-Boye D. Human bronchial fibroblast exhibit a mesenchymal stem cell phenotype and multilineage differentiating potentialities. Lab Invest. 2005;85:962-971.
- Shi S, Bartold P, Miura M, Seo B, Robey P, Gronthos S. The efficacy of mesenchymal stem cells to regenerate and repair dental structures. Orthod Craniofac Res. 2005;8:191-199.
- Krampera Ma, Pizzolo G, Aprili G, Franchini M. Mesenchymal stem cells for bone, cartilage, tendon and skeletal repair. Bone. 2006;39:678-683.
- Krampera Mb, Pasini A, Pizzolo G, Cosmi L, Romagni S, Annunziato. Regenerative and immunomodulatory potential of mesenchymal stem cells. Curr Opin Pharmacol. 2006;6:435-441.
- Kassem M, Kristiansen M, Abdallah B. Mesenchymal Stem Cells:Cell biology and potential use in therapy. Basic Clin Pharmacol Toxicol. 2004; 95:209-214.
- Mankani MH, Kuznetsov SA, Fowler B, Kingman A, Robey PG. In vivo bone formation by human bone marrow stromal cells: effect of carrier particle size and shape. Biotechnol Bioeng. 2001;72:96-107.
- Tsuchida H, Hashimoto J, Crawford E, Manske P, Lou J. Engineered allogeneic mesenchymal stem cells repair femoral segmental defect in rats. J Orthop Res. 2003;21:44-53.
- Mazhari R, Hare JM. Mechanisms of action of mesenchymal stem cells in cardiac repair: potential influences on the cardiac stem cell niche. Nat Clin Pract Cardiovasc Med. 2007;4:S21-S26.
- De Bari C, Dell’ accio F, Vandenabeele F, Vermeesch IR, Raymackers JM, Luyten FP. Skeletal muscle repair by adult human mesenchymal stem cells from synovial membrane. Blood Rev. 2006;200:161-171.
- Tse WT, Pendleton JD, Beyer WM, Egalka MC, Guinan EC. Suppresion of allogenic T-cell proliferation by human marrow stromal cells: implications in transplantation. Transplantation. 2003;75:389-397.
- Le Blanc K, Tammik I, Sundberg B, Haynesworth SE, Ringden O. Mesenchymal stem cells inhibit and stimulate mixed lymphocyte cultures and mitogenic responses independently of the major histocompatibility complex. Scand J Immunol. 2003;57:11-20.
- Aggarwal S, Pittenger MF. Human mesenchymal stem cells modulate allogenic immune cell responses. Blood. 2005;105:1815-1822.
- Zhang W, Ge W, Li C, You S, Liao I, Han Q et al. Effects of mesenchymal stem cells on differentiation, maduration, and function of human monocyte-derived dendritic cells. Stem Cells Dev. 2004;13:263-271.
- Maurillo L, Del Poeta G, Venditti A, Bucciana F, Battaglia A, Santinelli S et al. Quantitative analisis of Fas and Bcl-2 expression in haematopoietic precursors. Haematologica. 2001;86:237-243.
- Chang Y, Tseng C, Hsu I, Hsieh T, Hwang S. Characterization of two populations of mesenchymal progenitor cells in umbilical cord blood. Cell Biol Int. 2006;30:495-499.
- Kortesidis A, Zannettino A, Isenmann S, Shi S, Lapidot T, Gronthos S. Stromal-derived factor-1 promotes the growth, survival, and development of human bone marrow stromal cells. Blood. 2005;105:3793-3801.
- Gonçalves R, Da Silva C, Cabra J, Zanjani E, Almeida-Porada G. A Stro-1+ human universal stromal feeder layer to expand/maintain human bone marrow hematopoietic stem/progenitor cells in a serum-free culture system. Exp Hematol. 2006;34:1353-1359.
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- DOI: http://dx.doi.org/10.22490/24629448.380
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