Polimorfismos de nucleótido simple en hormonas asociadas al crecimiento muscular en ovinos criollos colombianos

Polimorfismos de nucleótido simple en hormonas asociadas al crecimiento muscular en ovinos criollos colombianos

Objective. To determine the Single Nucleotide Polymorphisms (SNPs) present in growth hormone (GH) and insulin like growth factor 1 (IGF-1) genes and their association with muscle growth in Colombian Creole hair sheep. Materials and methods. A population of 100 sheep was selected, from three different regions: Andean valleys, Piedemonte Llanero and Córdoba department, subjected to different production systems. Polymorphisms identification was determined by the Polymerase Chain Reaction (PCR) and Single Chain Conformation Polymorphism (SSCP) techniques. Results. AA, AB and BB genotypes were identified for these genes. Allele frequencies were defined for the GH and IGF-1 (IGFov-1, IGF1ov-2 and IGF1ov-3) markers of 58.9, 36.87, 53.76 and 56.81% for allele A, respectively, and 41.41, 63.13, 46.24 and 43.18% for allele B,
respectively. Genotypic frequencies were also determined for each marker at the population level, calculated from the Hardy-Weinberg equilibrium with a Fis correlation analysis. Conclusion. The selected markers present a high level of homology in the selected population, and it was determined that there is a high percentage of heterozygous individuals based on the markers evaluated. 

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