Astaxanthin production under stress factors using a 5L laboratory scale bioreactor
Producción de astaxantina bajo factores de estrés utilizando un biorreactor a escala de laboratorio de 5 L
NOVA by http://www.unicolmayor.edu.co/publicaciones/index.php/nova is distributed under a license creative commons non comertial-atribution-withoutderive 4.0 international.
Furthermore, the authors keep their property intellectual rights over the articles.
Perfil Google Scholar
Show authors biography
Introduction. Haematococcus pluvialis is a microalgae that produces astaxanthin, a beta-ca-rotene and antioxidant widely used in industry. In order to obtain a higher production of astaxanthin, the objective was to use different stress factors, in a 5-liter laboratory-scale bioreactor. Methodology. The microalgae was cultivated in the RM medium, pH 6.8, temperature 20 ± 2oC, filtered air, illumination with white lamps 20h light/4h darkness, irradiance 70 μE m−2s−1, different concentrations of sodium acetate and chloride of sodium. Cell growth, morphological changes and quantification of astaxanthin and chlorophyll were determined by spectrophotometry. Statistical analysis was performed using ANOVA (95%). Results. Using 0.299 mg/L of sodium acetate a cell growth of 2.0 x 104 Cel/mL and an astaxanthin concentration of 2.530 μg/mL were obtained, while with 1.6 mg/L of sodium acetate the cell growth It was 3.5 x 104 Cel/mL and an astaxanthin concentration of 1.9 μg/mL. The treatment to which 1.6 g L of sodium acetate and 6.4 g/L of sodium chlo-ride were added showed the highest astaxanthin production, 7.3 μg/ml. Treatment with 0.320 g/L sodium acetate + 1.28 g/L sodium chloride showed the highest cell growth with 1.64x105 cells/ml. Conclusion. This research highlights the importance of initially culti-vating the microalgae using the 5-liter Tecferm bioreactor and, after its exponential phase, subjecting it to stress factors with sodium acetate and sodium chloride, thus achieving the highest production of 7.325 μg / ml astaxanthin.
Article visits 202 | PDF visits 173
Downloads
1. Jeon YC, Cho CW, Yun YS. Combined effects of light intensity and acetate concentration on the growth of unicellular microalga Haematococcus pluvialis. Enzyme and Microbial Technology. 2006 Jul 3; 39(3):490-5.
2. Wang N, Guan B, Kong Q, Sun H, Geng Z, Duan L. Enhancement of astaxanthin production from Haematococcus pluvialis mutants by three-stage mutagenesis breeding. Journal of biotechnology. 2016 Oct 20; 236:71-7.
3. Wang, Junfeng, et al. Effect of initial biomass density on growth and astaxanthin production of Haematococcus pluvialis in an outdoor photobioreactor. Journal of applied phycology 2013. 25.1: 253-260.
4. Giannelli, Luca, et al. Effects of temperature on the astaxanthin productivity and light harvesting characteristics of the green alga Haematococcus pluvialis. Journal of bioscience and bioengineering 2015.119.3: 345-350.
5. García Martin Laura. Producción biotecnológica de astaxantina a partir Haematococcus pluvialis. 2018. Universidad Colegio Mayor de Cundinamarca. 86p.
6. Cifuentes A, González M, Vargas S, Hoeneisen M, González N. Optimization of biomass, total carotenoids and astaxanthin production in Haematococcus pluvialis Flotow strain Steptoe (Nevada, USA) under laboratory conditions Biol Res. 2003. 36: 343-357.
7. Ramírez D. Evaluación del crecimiento y producción de astaxantina por Haematococcus pluvialis en un fotobiorreactor tipo airlift. Tesis. Universidad Nacional de Colombia. 2013. [Citado 2019 Febrero 25]. Disponible en: http://www.bdigital.unal.edu.co/11205/1/300061.2013.pdf
8. Tocquin P, Fratamico A, Franck F. Screening for a low-cost Haematococcus pluvialis medium reveals an unexpected impact of a low N/P ratio on vegetative growth. Journal of applied phycology. 2012 Jun 1; 24(3):365-73.
9. Wan, Minxi, et al. The effect of temperature on cell growth and astaxanthin accumulation of Haematococcus pluvialis during a light–dark cyclic cultivation. Bioresource technology 2014. 167: 276-283.
10. Niño-Castillo CM, Rodríguez-Rivera FC, Díaz LE, Lancheros-Díaz AG. Evaluation of Cell Growth Conditions for the Astaxanthin Production as of Haematococcus pluvialis Microalgae. Nova. 2017 Dec; 15(28):19-31.
11. Park, J. C., Choi, S. P., Hong, M. E., & Sim, S. J. Enhanced astaxanthin production from microalga, Haematococcus pluvialis by two-stage perfusion culture with stepwise light irradiation. Bioprocess and biosystems engineering. 2014; 37(10), 2039-2047.
12. González MA, Cifuentes AS, Gómez PI. Growth and total carotenoid content in four Chilean strains of Haematococcus pluvialis Flotow, under laboratory conditions. Gayana. Botanica 2009. 66(1), 58.
13. Ranga R, Sarada A, Baskaran V, Ravishankar G. Identification of Carotenoids from Green Alga Haematococcus pluvialis by HPLC and LC-MS (APCI) and Their Antioxidant Properties. J. Microbiol. Biotechnol. 2009. 19:1333–1341
14. Shakhmatov AS, Pavlovskiy EV, Paukov AG. Desmid algae (Charophyta: Conjugatophyceae) of Ekaterinburg, Middle Urals, Russia. Folia Cryptogamica Estonica. 2018 May 29; 55:7-15.
15. Woong C,Jeon Y, Sang Y. Combined effects of light intensity and acetate concentration on the growth of unicellular microalga Haematococcus pluvialis. Rev Enzyme and Microbial Technology. Corea del Sur.2006 Julio. [accessed Mar 01 2018]. Disponible en:
https://www.researchgate.net/publication/228353480_Combined_effects_of_light_intensity_and_acetate_concentration_on_the_growth_of_unicellular_microalga_Haematococcus_pluvialis.
16. Infant Santhose B, Elumalai S, Rajesh Kanna G. Airlift photobioreactor cultivation of a new strain of Haematococcus pluvialis collected from high altitude regions of Himalayas. International Journal of Science and Research. 2014; 3(10):2289-92.
17. Camacho Kurmen, J. E., González, G., & Klotz, B. Astaxanthin Production in Haematococcus pluvialis under different stress conditions. Nova, 2013. 11(19), 94-104.
18. Imamoglu E, Sukan FV. Effect of Different Culture Media and Light Intensities on Growth of Haematococcus pluvialis. International journal of natural and engineering sciences. 2007; 1(3):5–9 doi: 10.1007/s11738-002-0058-9
19. Leiton Arcos Y. A. Producción de Haematococcus pluvialis en el biorreactor Tecferm de 5 L en medios de cultivo RM y BBM. 2018. Universidad Colegio Mayor de Cundinamarca. 91 p.
20. Goksan T, Ak I, Gokpinar S. An alternative approach to the traditional mixotrophic cultures of Haematococcus pluvialis Flotow (Chlorophyceae). Journal of microbiology and biotechnology. 2010 Sep; 20(9):1276-82.
21. Infant Santhose B, Elumalai S, Rajesh Kanna G. Airlift photobioreactor cultivation of a new strain of Haematococcus pluvialis collected from high altitude regions of Himalayas. International Journal of Science and Research. 2014. 3(10):2289-92.
22. Su Y, Wang J, Shi M, Niu X, Yu X, Gao L, Zhang X, Chen L, Zhang W. Metabolomic and network analysis of astaxanthin-producing Haematococcus pluvialis under various stress conditions. Bioresource technology. 2014 Oct 1; 170:522-9.
23. Vidhyavathi R, Venkatachalam L. Regulation of carotenoid byosinthetic genes expression and carotenoid accumulation in the green alga Haematococcus pluvialis under nutrient stress conditions. Journal of Experimental Botany. 2008. 59:1409-1418
24. Wayama M, Ota S, Matsuura H, Nango N, Hirata A, Kawano S. Three-dimensional ultrastructural study of oil and astaxanthin accumulation during encystment in the green alga Haematococcus pluvialis. PloS one. 2013 Jan 11; 8(1):e53618.
25. Domínguez-Bocanegra AR, Legarreta IG, Jeronimo FM, Campocosio AT. Influence of environmental and nutritional factors in the production of astaxanthin from Haematococcus pluvialis. Bioresource technology. 2004 Apr 1; 92(2):209-14.
26. Vidhyavathi, R., Sarada, R., & Ravishankar, G. A. Expression of carotenogenic genes and carotenoid production in Haematococcus pluvialis under the influence of carotenoid and fatty acid synthesis inhibitors. Enzyme and Microbial Technology. 2009; 45(2), 88-93.
27. Amos R. Handboook of Microalga. Culture Biotechnology and applied Phycology. Blackwell publishing. India. 2005
28. Vásquez Perea, Y., Villamil Poveda, J., Sánchez Leal, L., & Lancheros Díaz, A. Evaluación de un sistema de medio fijo como soporte para una película microbiana capaz de reducir Cr (VI) de lodos residuales de curtiembres. Nova. 2014. 12(21). https://doi.org/10.22490/24629448.996
29. Culture Colletion of Algae at The University at Austin (UTEX). Composición de medios para microalgas. [Internet]. . [Citado 2019 septiembre 20]. Disponible en: https://utex.org/products/volvox-medium
Information
Keywords
