Caracterización de los procesos de obtención, transporte, procesamiento y criopreservación de las muestras de sangre de cordón umbilical, obtenidas de enero de 2006 a febrero de 2008 en el banco de células Stem de Colombia

Luz Mabel Ávila Portillo et al.

doi:10.22490/24629448.418

Characterization of the Processes of Obtaining, Transporting, Processing and Cryopreservation of Samples of Umbilical Cord Blood Obtained from January 2006 to February 2008 in Colombia Stem Cells Bank

Caracterización de los procesos de obtención, transporte, procesamiento y criopreservación de las muestras de sangre de cordón umbilical, obtenidas de enero de 2006 a febrero de 2008 en el banco de células Stem de Colombia

Published 2009-06-30

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Vol. 7 No. 11 (2009)

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Artículo Original

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Characterization of the Processes of Obtaining, Transporting, Processing and Cryopreservation of Samples of Umbilical Cord Blood Obtained from January 2006 to February 2008 in Colombia Stem Cells Bank. (2009). NOVA, 7(11). https://doi.org/10.22490/24629448.418

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https://doi.org/10.22490/24629448.418

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NOVA by http://www.unicolmayor.edu.co/publicaciones/index.php/nova is distributed under a license creative commons non comertial-atribution-withoutderive 4.0 international.

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Luz Mabel Ávila Portillo et al.

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Cell viability

Cryopreservation

Myeloid leukemia

Stem cells

Umbilical cord

Umbilical cord blood has gone from being a biological waste to being a potential source of hematopoietic stem cells with regenerative capacity, making, processing and cryopreservation after collection under conditions that ensure their use is key to a successful transplant. The storage of hematopoietic stem cells from umbilical cord blood banks is conducted in both public and private character. Our objective was to analyze the experience of a bank of umbilical cord blood in Colombia, which characterizes the processes of production, transportation, processing and cryopreservation. To do this, we designed a case-series study, which analyzed 1080 records from the database of Stem Cell Bank of Colombia SA.

This analysis showed that the time elapsed before the processing of the samples was less than 12.7 ± 10.3 hours and the average volume from 68.2 ± 27.6 mL, the initial count of nucleated cells averaged 1.03 x 109 cm / mm 3, with an absolute value of cryopreserved CD34 + of 161.7 ± 199.7 cell / uL, the percentage of CD34 + cells from the samples was 0.228%, 88.6% of the studied samples had a viability rate higher than 95%, 21 samples corresponding to 1.9% had positive blood cultures and 100% of serological tests for infectious diseases were negative.

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DOI: http://dx.doi.org/10.22490/24629448.418