Cell Cycle by Gram Stain and Modified Fluorescence in Bacteria Resembling Morfotintorial Neisseria Gonorrhoeae Isolated from Anal and Urethral Samples
Ciclo celular por Gram y tinción de fluorescencia modificada en bacterias con aspecto morfotintorial semejante a Neisseria gonorrhoeae aisladas de muestras perianales y uretrales
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Cell cycle was evaluated by Gram stain and modified fluorescence in bacteria isolated from anal and urethral samples with observation diplococcus smear-negative or fluorescent orange diplococcus and negative culture for Neisseria gonorrhoeae in patients attending Dr. Archimedes Fuentes Serrano’s STI-AIDS ambulatory practice in Cumana, Venezuela, with the aim of showing that the isolated bacteria, Gram characteristics and fluorescence morfotintoriales like diplococci, are associated with cell cycle. Microscopic study was made of the cell cycle from synchronous cultures at time intervals of 5 minutes for 2 hours, taking aliquots, fixing in ethanol, and extended by coloring with Gram and fluorescence.
Escherichia coli, Enterobacter aerogenes, Klebsiella pneumoniae, Citrobacter koseri, Staphylococcus epidermidis, S. saprophyticus, b-hemolytic Streptococcus group A no-no B and Enterococcus faecalis were identified. Gram-positive cocci were the ones that expressed characteristics of diplococcus morfotintorial negative rods. b-hemolytic Streptococcus of 0-15 min, E. 60-80 min f aecalis, S. saprophyticus at 10 and 35, S. epidermidis to 0,15, 20, 35, 40, 70, 90 and 95 min. Staphylococcus fluorescence , visualized as diplococcus fluorescent orange, green and yellow were observed. Color varies cyclically, starting as yellow, through orange and then green. S. saprophyticus from 40-120 min was even observed fluorescent yellow. S. epidermidis even at 50, 60 and 65 min were visualized as fluorescent orange diplococcus. In conclusion, the gram-positive cocci show gram-negative phase and diplococcus fluorescent orange on their cell cycle.
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- Donachie W. What is the minimum number of dedicated functions required for a basic cell cycle? Curr Opin Genet Dev. 1992;2:792-798.
- Donachie W. The cell cycle of Escherichia coli. Annu Rev Microbiol. 1993;47:199-230.
- Albarado L, Flores E. Evaluación de la coloración diferencial de fluorescencia modificada en Pseudomonas spp. aisladas de suelo. Kasmera. 2008;36:17-27.
- Angert E. Alternatives to binary fission in bacteria. Nat Rev Microbiol. 2005;3:214-224.
- Weart R, Levin P. Growth rate-dependent regulation of medial FtsZ ring formation. J Bacteriol. 2003;185:2826-2834.
- Michie KA, Löwe J. Dynamic filaments of the bacterial cytoskeleton. J. Annu Rev Biochem. 2006;75:467-492.
- Margolin W. FtsZ and the division of prokaryotic cells and organelles. Nat Rev Mol Cell Biol. 2005;6:862-871.
- Midigan T, Martinko J, Parker J. Brock. Biología de los Microorganismos. Ed. 8ª. Madrid: Prentice-Hall; 1998.
- Dubytska L, Godfrey H, Cabello F. Borrelia burgdorferi ftsZ Plays a Role in Cell Division. Bacteriol. 2006:188:1969–1978.
- Tobiason D, Seifert H. The obligate human pathogen, Neisseria gonorrhoeae, is polyploid. PLoS Biol. 2006;4:1069-1078.
- Manavi K, Young H, Clutterbuck D. Sensitivity of microscopy for the rapid diagnosis of gonorrhoea in men and women and the role of gonorrhea serovars. Int J STD AIDS. 2003;14:390-394.
- CDC: Centers for Disease Control and Prevention. Screening tests to detect Chlamydia trachomatis and Neisseria gonorrhoeae infections. MMWR. 2002;51(RR15):1-27.
- Unemo M, Savicheva A, Budilovskaya O, Sokolovsky E, Larsson M, Domeika M. Laboratory diagnosis of Neisseria gonorrhoeae in St Petersburg, Russia: inventory, performance characteristics and recommended optimisations. Sex Transm Infect. 2006;82:41-44.
- Lai-King Ng, Martin I. The laboratory diagnosis of Neisseria gonorrhoeae. Can J Infect Dis Med Microbiol. 2005;16:15-25.
- Flores E, Albarado L, Thomas D, Lobo A. Comparación de la tinción fluorescencia modificada y Gram, en muestras urogenitales y perianales de pacientes asistidos en el área de Infecciones de Transmisión Sexual del Ambulatorio Arquímedes Fuentes, Cumaná estado Sucre. Salus. 2008;12:29-35.
- Flores E, Albarado L. Tinción diferencial de fluorescencia modificada en el diagnóstico de gonorrea. En: XIV Congreso Panamericano de Infectología. Sao Paulo: Office Editora e Publicidade Ltda; 2009. p. 67.
- Koneman E, Allen S, Janda W, Schreckenberger P, Winn W. Diagnóstico microbiológico. Texto y atlas color. Ed. 5ª. Buenos Aires: Editorial Médica Panamericana; 1999.
- Salguero I. Replicación cromosómica en presencia de una nucleósido difosfato reductasa codificada por el alelo nrdA101 de Escherichia coli [tesis doctoral]. Badajoz: Servicio de publicaciones, Universidad de Extremadura; 2007.
- Stainer R, Igraham J, Wheelis M, Painter P. Microbiología. Ed. 2ª. Barcelona: Editorial Reverte S.A; 1991
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