Vitamin D estimate in women with osteopenia and osteoporosis inCundinamarca, Colombia, by means of extraction in solid phase, liquid chromatography ofhigh resolution and multivaried analysis

Estimación de Vitamina D en mujeres con osteopenia y osteoporosis en Cundinamarca-Colombia, por medio de extracción en fase sólida, cromatografía líquida de alta resolución y análisis multivariado

Open | Download
PDF (Spanish) FLIP HTML (Spanish)
Issue
Vol. 1 No. 1 (2003)
Section
Artículo Original
How to Cite
Vitamin D estimate in women with osteopenia and osteoporosis inCundinamarca, Colombia, by means of extraction in solid phase, liquid chromatography ofhigh resolution and multivaried analysis. (2014). NOVA, 1(1). https://doi.org/10.22490/24629448.1055
doi

https://doi.org/10.22490/24629448.1055
Dimensions
PlumX
license

Licencia Creative Commons

NOVA by http://www.unicolmayor.edu.co/publicaciones/index.php/nova is distributed under a license creative commons non comertial-atribution-withoutderive 4.0 international.

Furthermore, the authors keep their property intellectual rights over the articles.

 

Magda Guevara et al.
Universidad Colegio Mayor de Cundinamarca, Bogotá, Colombia.
    Show authors biography
    At present great interest in the mineral metabolism exists and the role of the vitamin D 3, by its importance in the understanding of the pathogenesis of the Osteoporosis and consequently by its preventive and therapeutic applications. The quantification of the Vitamin D implies various previous steps: separation, extraction and  division that require the development of diverse methods seeking accuracy, precision, rapidity and versatility. The present study had as an objective to quantify the concentrations from serum of the main metabolites of the vitamin D 3 : 24-hidroxicolecalciferol, 24,25-dihidroxicolecalciferol and 1,25 dihidroxicolecalciferol aforesaid as vitamin D3 total in 50 patients with osteopenia or osteoporosis. The trial was carried out for a system of HPLC. The percent recovery post-extraction went of the 85%, the sensibility for the 1,25 dihidroxicolecalciferol went of 12.37 pg/ml and of the colecalciferol of 25 pg/ml. The average of Vitamin D 3 total went of 2072 ng/ ml with an interval from 6.3 to 10600 ng/ml and was found differentiates significant in the Vitamin D concentration averages among women with DMO normal and the ones that had al less osteopenia in hip (p<0.04), nevertheless this difference was not significant in column. A significant difference of average concentrations of vitamin was found D among cases with and without antecedent of fracture (p=0.12). The relation of the concentrations of vitamin is discussed D with the bony mineral values of density and sociodemographic, biological, and clinical variables.

    Article visits 325 | PDF visits 169

    Downloads

    Download data is not yet available.
    1. URL:http://www.medspain.com/ant/n14_jul00/vit.htm
    2. Ilczyszyn GR, Gurí JC. Las virtudes de la vitamina D contra la osteoporosis. 1999. Disponible en: URL: http://www.healthig.com/vitaminas/vitaminas4.html
    3. Rubio LE, Morán M, Talbot JR, ZanchettA JR. Concentración sérica de 25 (ho) vitamina D en sujetos jóvenes y añosos residentes en buenos aires Trabajo Original. The Official Journal of the Pan American Osteoporosis Foundation, Instituto de Investigaciones Metabólicas (IDIM), Universidad del Salvador. Disponible en: URL: http://www.osteored.com.ar/web/osteored.nsf
    4. La vitamina D y sus principales funciones. 1999. Disponible en: URL: http://www.forever-life.com/vitaminaD.htm
    5. Koller T. La Vitamina D: el «beso del sol» para nuestra salud, bloqueada por la computadora!. 2000. Disponible en: URL: http://cuerpodiet.com/tusvitaminas/vitaminaD.htm
    6. En buenas manos. Vitamina D. Portal de salud y terapias naturales. Disponible en: URL: http://www.enbuenasmanos.com/ARTICULOS/ muestra.asp?art=351&unic=1
    7. La importancia de la vitamina D en los recién nacidos. Salud. Disponible en: URL: http://www.zonadiet.com/salud/vit-dlactancia.htm
    8. University of Glasgow. 1,25 Dihydroxy Vitamin D. 1996. Disponible en: URL: http://www.pdg.cnb.uam.es/cursos/Barcelona2002/pages/Farmac/Immunoassays/125DWeb.htm
    9. Timothy A, et al. A Microassay for 1,25-Dihydroxyvitamin D Not Requiring High Performancen Liquid Chromatography: Application to Clinical Studies. Journal of Clinical Endocrinology and Metabolismo 1984;58(1):91-8.
    10. Díaz L, Cariño C, Méndez I. La vitamina D: implicaciones en la salud y el embarazo. Revista de Investigación Clínica 2001;53(1):77-85.
    11. Ferrero M, Fernández S. Vitamina D y terapias contra las enfermedades neurodegenerativas químicos del principado. 1994;(34). Colegio Oficial de Químicos de Asturias y León 2000. Disponible en: URL: http://www.alquimicos.com/quimprin/34/vitamina.html
    12. Beyond discovery. Para aclarar el enigma de la vitamina D. Vínculo de la vitamina D con el control del calcio. Disponible en: URL: http://www7.nationalacademies.org/spanishbeyonddiscovery/bio_008721-06.html
    13. Cravioto MdC. Vitamina D placentaria: síntesis, regulación e implicaciones clínicas. Revista de Investigación Clínica 2000;52(6):692-4.
    14. Mouriño Mosquera JA, et al. Diseño, síntesis e implicaciones biológicas y terapéuticas de nuevos análogos de la vitamina D. Facultad de Química, Santiago de Compostela. 15706 Disponible
    15. en: URL: http://qoweb.usc.es/investigacion/grupos/AM/indice.html
    16. Mangelsdorf DJ, Evans R. La vitamina D sería fundamental para prevenir el cáncer de colon. Instituto Médico Howard Hughes (HHMI). Science 2002. Disponible en: URL: http://www.hhmi.org/news/mangelsdorf2-esp.html.
    17. Derivados vitamina D. Psoriasis. Disponible en: URL: http://www.humano.ya.com/psoriasis/VitaminaD.htm 11.Noticias sobre la vitamina D.
    18. Taverna MJ, et al. Taq I polymorphism of the vitamin D receptor and risk of severe diabetic retinopathy. Diabetología 2002;45:436-42.
    19. Noticias sobre la vitamina D. Disponible en: URL: http://www.dietasalamedida.com/todito/noticias/noticias_sobre_la_vitamina_d.asp.
    20. Faes farma. Área de comunicación desarrollo de noticia. Symposium satélite sobre actualizaciones en la clínica geriátrica: depresión/osteoporosis. Disponible en: URL: http://www.faes.es/area_ comunicación/desarrollo_noticia/010200.html .
    21. Fonendo. Nutrición.Ala vejez... nueces!!! Disponible en: URL: http://www.fonendo.com/noticias/40/2000/09/5.shtm
    22. Asociación Colombiana de Osteología y Metabolismo Mineral. Asociación Colombiana de Osteoporosis (ACOMM). Conferencia de consenso diagnóstico y manejo de la osteoporosis.
    23. Congreso Colombiano de Osteoporosis; 2001. Disponible en: URL: http://www.paof.org/consensos11.pdf
    24. Ardila E. Características de la Osteoporosis en Colombia. Revista de la Asociación colombiana de Gerontología y Geriatría 2002;16(3).
    25. Ching Pontón RB, et al. Factores de riesgo para osteoporosis y osteopenia en 707 mujeres seleccionadas en Bogotá. Reemo 2001;10(3).
    26. Prockop D. The genetic trail of osteoporosis. The New England Journal of Medicine 1996;338(15):1061-2.
    27. Hollis BW, Kamerud JQ, Kurkowski A, Beaulieu J, Napoli JL. Quantification of circulating 1,25-dihydroxivitamin D by radioinmunoassay with an I 25I-labeled tracer. Clinical Chemistry
    28. ;42(4):586-92
    29. Koyama H, Prahl JM, Uhland A, Nanjo M, Inaba M, et al. A New, Highly Sensitive Assay for 1,25-Dihyroxyvitamin D Not Requiring High-Performance Liquid chromatography: Application of Monoclonal Antibody against Vitamin D Receptor to Radioreceptor Assay. Analytical Biochemistry 1992;205:213-9
    30. Hollis BW. Assay of Circulating 1,25-Dihydroxyvitamin D Involving a Novel Single- Cartridge Extraction and Purification Procedure. Clinical Chemistry 1986;32(11):2060-3.
    31. P. Universidad Católica de Chile. Osteomalacia. Apuntes de reumatología. Disponible en: URL: http://escuela.med.puc.cl/ publ/reumatologia/Apuntes/16Osteomalacia.html
    32. Hollis, B y Frank, N. Solid phase extraction system for d and its major metabolites in human plasma. Journal of Chromatography. Biomedical Applications 1985;343:43-9.
    33. Bolliger, H.R. En: Stahl E. Mun-layre Chromatography. Springen Verlag, Berlín, Heidelberg, NewYork, Londres:Academic Press. Inc.; 1965. p. 227-8
    34. Favus MJ. Primer on the Metabolic Bone Diseases and Disorders of Mineral Metabolism. 2nd ed. New York: Raven Press; 1993.
    35. Heaney Rp. Bone Mass, Nutrition and other lifestyle factors. The American Journal of Medicine 1993;95(Suppl 5ª):29s-31s.
    36. Delmas PD. Biochemical Markers of Bone turnover I: Theoretical Considerations and Clinical Use in Osteoporosis. TheAmerican Journal of Medicine 1993;95(Suppl 5ª):11-6.
    37. -------------------------------------------------------------------------------
    38. DOI: http://dx.doi.org/10.22490/24629448.1055
    Tutorials

    Autors:

    How send article?

    Evaluator pair

    How to evaluate an article?

    Information

    Keywords

    Google Scholar profile

    Is part of
     

    Current Issue

    Sistema OJS 3.4.0.5 - Metabiblioteca |