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Inmunogenicidad del antígeno ESAT-6 de Mycobacterium tuberculosis en monos búho

Mycobacterium tuberculosis ESAT-6 antigen immunogenicity in Owl Monkeys



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Inmunogenicidad del antígeno ESAT-6 de Mycobacterium tuberculosis en monos búho. (2006). NOVA, 4(5). https://doi.org/10.22490/24629448.344

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NOVA por http://www.unicolmayor.edu.co/publicaciones/index.php/nova se distribuye bajo una Licencia Creative Commons Atribución-NoComercial-SinDerivar 4.0 Internacional.

Así mismo,  los autores mantienen sus derechos de propiedad intelectual sobre los artículos.  

Martha Calderon
    Carlos Parra-López
      Rosalba Alfonso
        Paola Barato
          Diana Giraldo
            Martha Pinto
              Manuel Patarroyo

                La ̇única vacuna disponible contra la tuberculosis es la cepaMycobacterium bovisBCG, que ofrece unaeficacia protectiva variable (0%-80%), siendo urgente un nuevo agente profil·ctico. Se han evaluado diversos candidatos a vacuna contra este patogeno, en los modelos animales de experimentaciÛn convencionales(murino, cobayo, conejo), obteniÈndose informaciÛn b·sica sobre el efecto de la vacuna en la carga bacterialfrente a un reto infeccioso, asÌ como tambiÈn la reducciÛn o prevenciÛn de la patologÌa en los pulmones uotros Ûrganos blanco; adem·s de los aspectos relacionados con la respuesta inmune hacia elMycobacteriumtuberculosis. Los primates no humanos tienen ventajas sobre los modelos convencionales en la evaluaciÛn devacunas, de hecho se ha verificado el comportamiento de agentes terapÈuticos en humanos despuÈs de habersido medida la capacidad protectiva de Èstos en monos con tuberculosis inducida. Los primates mas estudia-dos en la infección por micobacterias son el cynomulgus, y el rhesus, observ·ndose que estos animalesmantienen la infección en un estado subclÌnico, muy similar a la tuberculosis humana donde el 90% de lapoblación infectada mantiene la infecciÛn en un estado latente. Dado que el modelo animal debe semejar el comportamiento de las proteÌnas estudiadas en el ser humano, el monoAotuspuede representar ventajas enla investigación de tuberculosis por ser un primate con aproximadamente un 90% de similitud al humano encuanto a las molÈculas del sistema inmune estudiadas hasta hoy. La proteÌna ESAT-6 de (early secretoryantigenic target 6 kD) deMycobacterium tuberculosises un componente minoritario del filtrado de cultivode corto tiempo (CFP), ha sido genÈtica y quÌmicamente caracterizada e induce una potente respuestainmunogÈnica del tipo TH1. Este antÌgeno es secretado durante la fase inicial de crecimiento siendo fuerte-mente reconocido por animales y humanos infectados porMycobacterium tuberculosis, este hecho haceque su inclusiÛn en una futura vacuna anti-tuberculosis por subunidades y en pruebas de inmunodiagnÛstico.En este trabajo se estudiÛ la respuesta inmune del monoAotusfrente al antÌgeno micobacteriano ESAT-6 enforma recombinante (rESAT-6). El antÌgeno fue inmunizado junto con el adyuvante Montanide 720 producien-do una fuerte respuesta humoral y celular, no solo hacia rESAT-6 sino tambiÈn hacia la proteÌna nativa pre-sente en el filtrado de medio de cultivo deMycobacterium tuberculosis.La respuesta celular fue medidapor la incorporaciÛn de [3H]timidina en ensayos de linfoproliferaciÛn. La respuesta humoral se analizÛ porensayos de ELISA e inmunoblot, obteniÈndose altos tÌtulos de anticuerpos (hasta de 1:12,800) dirigidos rESAT-6. Se demostrÛ la naturaleza multiepitope de este antÌgeno ya que en general pÈptidos que mapean la proteÌnafueron especÌficamente reconocidos tanto a nivel celular como humoral. Se observÛ adem·s variaciÛn en larespuesta a la vacunación entre animal y animal, siendo una desventaja para un modelo de experimentación en cuanto a la predicción de la respuesta. Este estudio se constituye en un primer paso de evaluaciÛn delmonoAotuscomo modelo animal en tuberculosis.


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                1. Colditz G, Brewer T, Berkey C, Wilson M, Burdick E, Fineberg H, Mosteller F: Efficacy of BCG vaccine in the prevention of tuberculosis. Metaanalysis of the published literature. Jama 271:698-702, 1994
                2. Fine PE: Variation in protection by BCG: implications of and for heterologous immunity. Lancet 346:1339-1345, 1995
                3. Ginsberg AM: What.s new in tuberculosis vaccines? Bull World Health Organ 80:483-488, 2002
                4. . Cole ST, Brosch R, Parkhill J, Garnier T, Churcher C, Harris D, Gordon SV, Eiglmeier K, Gas S, Barry CE, 3rd, Tekaia F, Badcock K, Basham D, Brown D, Chillingworth T, Connor R, Davies R, Devlin K, Feltwell T, Gentles S, Hamlin N, Holroyd S, Hornsby T, Jagels K, Barrell BG, et al.: Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature 393:537-544, 1998
                5. . Tekaia F, Gordon SV, Garnier T, Brosch R, Barrell BG, Cole ST: Analysis of the proteome of Mycobacterium tuberculosis in silico. Tuber Lung Dis 79:329-342, 1999
                6. . Jungblut PR, Schaible UE, Mollenkopf HJ, Zimny-Arndt U, Raupach B, Mattow J, Halada P, Lamer S, Hagens K, Kaufmann SH: Comparative proteome analysis of Mycobacterium tuberculosis and Mycobacterium bovis BCG strains: towards functional genomics of microbial pathogens. Mol Microbiol 33:1103-1117, 1999
                7. . Domenech P, Barry CE, 3rd, Cole ST: Mycobacterium tuberculosis in the post-genomic age. Curr Opin Microbiol 4:28-34, 2001
                8. Covert BA, Spencer JS, Orme IM, Belisle JT: The application of proteomics in defining the T cell antigens of Mycobacterium tuberculosis. Proteomics 1:574-586, 2001
                9. Brennan PJ: Structure, function, and biogenesis of the cell wall of Mycobacterium tuberculosis. Tuberculosis (Edinb) 83:91-97, 2003
                10. Brennan PJ, Besra GS: Structure, function and biogenesis of the mycobacterial cell wall. Biochem Soc Trans 25:188-194, 1997
                11. Orme IM, Cooper AM: Cytokine/chemokine cascades in immunity to tuberculosis. Immunol Today 20:307-312, 1999
                12. Flynn JL, Chan J: Immunology of tuberculosis. Annu Rev Immunol 19:93-129, 2001
                13. Pym AS, Brodin P, Majlessi L, Brosch R, Demangel C, Williams A, Griffiths KE, Marchal G, Leclerc C, Cole ST: Recombinant BCG exporting ESAT-6 confers enhanced protection against tuberculosis. Nat Med 9:533-539, 2003
                14. Bao L, Chen W, Zhang H, Wang X: Virulence, immunogenicity, and protective efficacy of two recombinant Mycobacterium bovis bacillus Calmette-Guerin strains expressing the antigen ESAT-6 from Mycobacterium tuberculosis. Infect Immun 71:1656-1661, 2003
                15. Jackson M, Phalen SW, Lagranderie M, Ensergueix D, Chavarot P, Marchal G, McMurray DN, Gicquel B, Guilhot C: Persistence and protective efficacy of a Mycobacterium tuberculosis auxotroph vaccine. Infect Immun 67:2867-2873, 1999
                16. Kamath AT, Feng CG, Macdonald M, Briscoe H, Britton WJ: Differential protective efficacy of DNA vaccines expressing secreted proteins of Mycobacterium tuberculosis. Infect Immun 67:1702-1707, 1999
                17. Li Z, Howard A, Kelley C, Delogu G, Collins F, Morris S: Immunogenicity of DNA vaccines expressing tuberculosis proteins fused to tissue plasminogen activator signal sequences. Infect Immun 67:4780-4786, 1999
                18. Huygen K: On the use of DNA vaccines for the prophylaxis of mycobacterial diseases. Infect Immun 71:1613-1621, 2003
                19. Nor NM, Musa M: Approaches towards the development of a vaccine against tuberculosis: recombinant BCG and DNA vaccine. Tuberculosis (Edinb) 84:102-109, 2004
                20. Orme IM, McMurray DN, Belisle JT: Tuberculosis vaccine development: recent progress. Trends Microbiol 9:115-118, 2001
                21. Dannenberg AM Jr, Collins FM: Progressive pulmonary tuberculosis is not due to increasing numbers of viable bacilli in rabbits, mice and guinea pigs, but is due to a continuous host response to mycobacterial products. Tuberculosis (Edinb);81(3):229-42, 2001
                22. Baldwin SL, D.Souza C, Roberts AD, Kelly BP, Frank AA, Lui MA, Ulmer JB, Huygen K, McMurray DM, Orme IM:
                23. Evaluation of new vaccines in the mouse and guinea pig model of tuberculosis. Infect Immun 66:2951-2959, 1998
                24. Scanga CA, Mohan VP, Yu K, Joseph H, Tanaka K, Chan J, Flynn JL: Depletion of CD4(+) T cells causes reactivation of murine persistent tuberculosis despite continued expression of interferon gamma and nitric oxide synthase 2. J Exp Med 192:347-358, 2000
                25. Mogues T, Goodrich ME, Ryan L, LaCourse R, North RJ: The relative importance of T cell subsets in immunity and immunopathology of airborne Mycobacterium tuberculosis infection in mice. J Exp Med 193:271-280, 2001
                26. McMurray DN: Recent progress in the development and testing of vaccines against human tuberculosis. Int J Parasitol 33:547-554, 2003
                27. McMurray DN: A nonhuman primate model for preclinical testing of new tuberculosis vaccines. Clin Infect Dis 30 Suppl 3:S210-212, 2000
                28. Good RC: Diseases in Nonhuman Primates. In The Mycobacteria. A Sourcebook. Kubica G, Wayne L (eds). New York, Marcel Dekker. Inc., 1984, pp 903-924
                29. VandeBerg JL, Williams-Blangero S: Advantages and limitations of nonhuman primates as animal models in genetic research on complex diseases. J Med Primatol 26:113-119, 1997
                30. Walsh GP, Tan EV, dela Cruz EC, Abalos RM, Villahermosa LG, Young LJ, Cellona RV, Nazareno JB, Horwitz MA: The Philippine cynomolgus monkey (Macaca fasicularis) provides a new nonhuman primate model of tuberculosis that resembles human disease. Nat Med 2:430-436, 1996
                31. Capuano SV, III, Croix DA, Pawar S, Zinovik A, Myers A, Lin PL, Bissel S, Fuhrman C, Klein E, Flynn JL: Experimental Mycobacterium tuberculosis infection of cynomolgus macaques closely resembles the various manifestations of human M. tuberculosis infection. Infect Immun 71:5831-5844, 2003
                32. Langermans JA, Andersen P, van Soolingen D, Vervenne RA, Frost PA, van der Laan T, van Pinxteren LA, van den Hombergh J, Kroon S, Peekel I, Florquin S, Thomas AW: Divergent effect of bacillus Calmette-Guerin (BCG) vaccination on Mycobacterium tuberculosis infection in highly related macaque species: implications for primate models in tuberculosis vaccine research. Proc Natl Acad Sci U S A 98:11497-11502, 2001
                33. Baer JF: Husbandry and medical management of the owl monkey. In Aotus: The owl monkey Baer JF, Weller RE, Kakoma I (eds). New York, Academic Press Inc., 1994, pp 133-164
                34. Attanasio R, Pehler K, McClure HM: Immunogenicity and safety of Mycobacterium tuberculosis culture filtrate proteins in nonhuman primates. Clin Exp Immunol 119:84-91, 2000
                35. Pehler K, Brasky KM, Butler TM, Attanasio R: Mycobacterium tuberculosis-secreted protein antigens: immunogenicity in baboons. J Clin Immunol 20:306-316, 2000
                36. Berthet FX, Rasmussen PB, Rosenkrands I, Andersen P, Gicquel B: A Mycobacterium tuberculosis operon encoding ESAT-6 and a novel low-molecular-mass culture filtrate protein (CFP-10). Microbiology 144 ( Pt 11):3195-3203, 1998
                37. Gey Van Pittius NC, Gamieldien J, Hide W, Brown GD, Siezen RJ, Beyers AD: The ESAT-6 gene cluster of Mycobacterium tuberculosis and other high G+C Gram-positive bacteria. Genome Biol 2:RESEARCH0044, 2001
                38. Sorensen AL, Nagai S, Houen G, Andersen P, Andersen AB: Purification and characterization of a low-molecular-mass T-cell antigen secreted by Mycobacterium tuberculosis. Infect Immun 63:1710-1717, 1995
                39. Harboe M, Oettinger T, Wiker HG, Rosenkrands I, Andersen P: Evidence for occurrence of the ESAT-6 protein in Mycobacterium tuberculosis and virulent Mycobacterium bovis and for its absence in Mycobacterium bovis BCG. Infect Immun 64:16-22, 1996
                40. Renshaw PS, Panagiotidou P, Whelan A, Gordon SV, Hewinson RG, Williamson RA, Carr MD: Conclusive evidence that the major Tcell antigens of the Mycobacterium tuberculosis complex ESAT-6 and CFP-10 form a tight, 1:1 complex and characterization of the structural properties of ESAT-6, CFP-10, and the ESAT-6*CFP-10 complex. Implications for pathogenesis and virulence. J Biol Chem 277:21598-21603, 2002
                41. Skjot RL, Oettinger T, Rosenkrands I, Ravn P, Brock I, Jacobsen S, Andersen P: Comparative evaluation of low-molecular-mass proteins from Mycobacterium tuberculosis identifies members of the ESAT-6 family as immunodominant T-cell antigens. Infect Immun 68:214-220, 2000
                42. Mustafa AS, Oftung F, Amoudy HA, Madi NM, Abal AT, Shaban F, Rosen Krands I, Andersen P: Multiple epitopes from the Mycobacterium tuberculosis ESAT-6 antigen are recognized by antigen-specific human T cell lines. Clin Infect Dis 30 Suppl 3:S201-205, 2000
                43. Brandt L, Elhay M, Rosenkrands I, Lindblad EB, Andersen P: ESAT-6 subunit vaccination against Mycobacterium tuberculosis. Infect Immun 68:791-795, 2000
                44. Olsen AW, Hansen PR, Holm A, Andersen P: Efficient protection against Mycobacterium tuberculosis by vaccination with a single subdominant epitope from the ESAT-6 antigen. Eur J Immunol 30:1724-1732, 2000
                45. Mori T, Sakatani M, Yamagishi F, Takashima T, Kawabe Y, Nagao K, Shigeto E, Harada N, Mitarai S, Okada M, Suzuki K, Inoue Y, Tsuyuguchi K, Sasaki Y, Mazurek GH, Tsuyuguchi I: Specific Detection of Tuberculosis Infection with an Interferongamma Based Assay Using New Antigens. Am J Respir Crit Care Med, 2004
                46. Goodman M, Porter CA, Czelusniak J, Page SL, Schneider H, Shoshani J, Gunnell G, Groves CP: Toward a phylogenetic classification of Primates based on DNA evidence complemented by fossil evidence. Mol Phylogenet Evol 9:585-598, 1998
                47. Nino-Vasquez JJ, Vogel D, Rodriguez R, Moreno A, Patarroyo ME, Pluschke G, Daubenberger CA: Sequence and diversity of DRB genes of Aotus nancymaae, a primate model for human malaria parasites. Immunogenetics 51:219-230, 2000
                48. Daubenberger CA, Salomon M, Vecino W, Hubner B, Troll H, Rodriques R, Patarroyo ME, Pluschke G: Functional and structural similarity of V gamma 9V delta 2 T cells in humans and Aotus monkeys, a primate infection model for Plasmodium falciparum malaria. J Immunol 167:6421-6430, 2001
                49. Montoya GE, Vernot JP, Patarroyo ME: Partial characterization of the CD45 phosphatase cDNA in the owl monkey (Aotus vociferans). Am J Primatol 57:1-11, 2002
                50. Guerrero JE, Pacheco DP, Suarez CF, Martinez P, Aristizabal F, Moncada CA, Patarroyo ME, Patarroyo MA: Characterizing Tcell receptor gamma-variable gene in Aotus nancymaae owl monkey peripheral blood. Tissue Antigens 62:472-482, 2003
                51. Webster D, Hill AV: Progress with new malaria vaccines. Bull World Health Organ 81:902-909, 2003
                52. Espejo F, Bermudez A, Torres E, Urquiza M, Rodriguez R, Lopez Y, Patarroyo ME: Shortening and modifying the 1513 MSP-1 peptide.s alpha-helical region induces protection against malaria. Biochem Biophys Res Commun 315:418-427, 2004
                53. Rodriguez R, Moreno A, Guzman F, Calvo M, Patarroyo ME: Studies in owl monkeys leading to the development of a synthetic vaccine against the asexual blood stages of Plasmodium falciparum. Am J Trop Med Hyg 43:339-354, 1990
                54. Houghten RA: General method for the rapid solid-phase synthesis of large numbers of peptides: specificity of antigenantibody interaction at the level of individual amino acids. Proc Natl Acad Sci U S A 82:5131-5135, 1985
                55. Alfonso R, Romero RE, Diaz A, Calderon MN, Urdaneta G, Arce J, Patarroyo ME, Patarroyo MA: Isolation and
                56. identification of mycobacteria in New World primates maintained in captivity. Vet Microbiol 98:285-295, 2004
                57. Palomino JC, Portaels F: Effects of decontamination methods and culture conditions on viability of Mycobacterium ulcerans in the BACTEC system. J Clin Microbiol 36:402-408, 1998
                58. Espinosa AM, Sierra AY, Barrero CA, Cepeda LA, Cantor EM, Lombo TB, Guzman F, Avila SJ, Patarroyo MA: Expression, polymorphism analysis, reticulocyte binding and serological reactivity of two Plasmodium vivax MSP-1 protein recombinant fragments. Vaccine 21:1033-1043, 2003
                59. Bollag DM, Edelstein SJ: Immunoblotting. In Protein Methods.
                60. (ed). New York, John Wiley and sons, Inc, 1993, pp 181-211
                61. Andersen P, Andersen AB, Sorensen AL, Nagai S: Recall of longlived immunity to Mycobacterium tuberculosis infection in mice. J Immunol 154:3359-3372, 1995
                62. Ulrichs T, Munk ME, Mollenkopf H, Behr-Perst S, Colangeli R, Gennaro ML, Kaufmann SH: Differential T cell responses to Mycobacterium tuberculosis ESAT6 in tuberculosis patients and healthy donors. Eur J Immunol 28:3949-3958, 1998
                63. Brock I, Weldingh K, Lillebaek T, Follmann F, Andersen P: Comparison of a New Specific Blood Test and the Skin Test in Tuberculosis Contacts. Am J Respir Crit Care Med, 2004
                64. Brandt L, Oettinger T, Holm A, Andersen AB, Andersen P: Key epitopes on the ESAT-6 antigen recognized in mice during the recall of protective immunity to Mycobacterium tuberculosis. J Immunol 157:3527-3533, 1996
                65. Pollock JM, Andersen P: Predominant recognition of the ESAT-6 protein in the first phase of interferon with Mycobacterium bovis in cattle. Infect Immun 65:2587-2592, 1997
                66. Kanaujia GV, Motzel S, Garcia MA, Andersen P, Gennaro ML: Recognition of ESAT-6 sequences by antibodies in sera of tuberculous nonhuman primates. Clin Diagn Lab Immunol 11:222-226, 2004
                67. Morris S, Kelley C, Howard A, Li Z, Collins F: The immunogenicity of single and combination DNA vaccines against tuberculosis. Vaccine 18(20):2155-63, 2000
                68. Minion FC, Menon SA, Mahairas GG, Wannemuehler MJ: Enhanced murine antigen-specific gamma interferon and immunoglobulin G2a responses by using mycobacterial ESAT-6 sequences in DNA vaccines. Infect Immun 71:2239-2243, 2003
                69. Ravn P, Demissie A, Eguale T, Wondwosson H, Lein D, Amoudy HA, Mustafa AS, Jensen AK, Holm A, Rosenkrands I, Oftung F, Olobo J, von Reyn F, Andersen P: Human T cell responses to the ESAT-6 antigen from Mycobacterium tuberculosis. J Infect Dis 179:637-645, 1999
                70. Vincenti D, Carrara S, De Mori P, Pucillo LP, Petrosillo N, Palmieri F, Armignacco O, Ippolito G, Girardi E, Amicosante M, Goletti D: Identification of early secretory antigen target-6 epitopes for the immunodiagnosis of active tuberculosis. Mol Med 9:105-111, 2003
                71. Mustafa AS, Shaban FA, Al-Attiyah R, Abal AT, El-Shamy AM, Andersen P, Oftung F: Human Th1 cell lines recognize the Mycobacterium tuberculosis ESAT-6 antigen and its peptides in association with frequently expressed HLA class II molecules. Scand J Immunol 57:125-134, 2003
                72. Patarroyo ME, Romero P, Torres ML, Clavijo P, Moreno A, Martinez A, Rodriguez R, Guzman F, Cabezas E: Induction of protective immunity against experimental infection with malaria using synthetic peptides. Nature 328:629-632, 1987
                73. Collins WE, Galland GG, Sullivan JS, Morris CL: Selection of different strains of Plasmodium falciparum for testing bloodstage vaccines in Aotus nancymai monkeys. Am J Trop Med Hyg 51:224-232, 1994
                74. Diaz D, Naegeli M, Rodriguez R, Nino-Vasquez JJ, Moreno A, Patarroyo ME, Pluschke G, Daubenberger CA: Sequence and diversity of MHC DQA and DQB genes of the owl monkey Aotus nancymaae. Immunogenetics 51:528-537, 2000
                75. Pinzon-Charry A, Vernot JP, Rodriguez R, Patarroyo ME: Proliferative response of peripheral blood lymphocytes to mitogens in the owl monkey Aotus nancymae. J Med Primatol 32:31-38, 2003
                76. Chaparro PE, García I, Guerrero MI, León CI: Situación de la tuberculosis en Colombia. Biomédica 2004;24(Supl.):102-14, 2002.
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                78. DOI: http://dx.doi.org/10.22490/24629448.344
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