Influencia de la fuente de carbono sobre la expresión de proteínas AOX1-reguladas en Pichia pastoris

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Autores

Raúl A. Poutou Balkys E. Quevedo Henrry A. Córdoba Homero Sáenz Sáenz Luis A. Barrera

Resumen

La levadura Pichia pastoris constituye un excelente modelo para la expresión de proteínas heterólogas, lo que se refleja en el gran número de proteínas que han sido obtenidas en este modelo biológico, bajo el control del promotor AOX1. Esto implica que el número de peroxisomas y la enzima alcohol oxidasa es regulada como respuesta a la inducción por metabolitos como el metanol, el glicerol, el etanol, el acetato y vías metabólica como la b-oxidación. En esta revisión, se discuten aspectos relacionados con el metabolismo de estos compuestos y su posible influencia en la producción de proteínas recombinantes, específicamente la Iduronato 2- sulfato sulfatasa humana (IDSh).

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DOI: http://dx.doi.org/10.22490/24629448.21

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