EFECTO DEL FACTOR DE CRECIMIENTO FIBROBLÁSTICO DOS EN LA REDUCCIÓN DE LA SENESCENCIA EN CÉLULAS MADRE MESENQUIMALES AISLADAS DE GELATINA DE WHARTON
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Rubio Vargas, A., Alcázar, J. P., Lozano Trujillo, L. A., Garzón Perdomo, D. K., Bonilla Porras, A. R., González Gay, O. T., & Francis Turner, L. (2022). EFECTO DEL FACTOR DE CRECIMIENTO FIBROBLÁSTICO DOS EN LA REDUCCIÓN DE LA SENESCENCIA EN CÉLULAS MADRE MESENQUIMALES AISLADAS DE GELATINA DE WHARTON. NOVA, 20(38). https://doi.org/10.22490/24629448.6188
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Autores
Andrea Catalina Rubio Vargas
https://orcid.org/0000-0001-7544-7008
https://orcid.org/0000-0001-7544-7008
Jessica Paola Alcázar
https://orcid.org/0000-0002-5598-4436
https://orcid.org/0000-0002-5598-4436
Laura Alejandra Lozano Trujillo
https://orcid.org/0000-0002-9226-2926
https://orcid.org/0000-0002-9226-2926
Diana Katherine Garzón Perdomo
https://orcid.org/0000-0002-5101-3261
https://orcid.org/0000-0002-5101-3261
Angélica Rocío Bonilla Porras
https://orcid.org/0000-0002-9580-9915
https://orcid.org/0000-0002-9580-9915
Olivia Teresa González Gay
http://orcid.org/0000-0002-5026-1191
http://orcid.org/0000-0002-5026-1191
Liliana Francis Turner
https://orcid.org/0000-0002-9189-0402
https://orcid.org/0000-0002-9189-0402
Resumen
Las células madre mesenquimales han generado interés en la ingeniería de tejidos, debido a sus propiedades proliferativas y capacidad de reparación de tejidos, sin embargo, para un trasplante exitoso, es necesario aumentar el número de células mediante un cultivo in-vitro. Durante este proceso la capacidad proliferativa disminuye, provocando cambios en la morfología y funcionalidad celular y afectando la viabilidad del cultivo, este estado se conoce como senescencia celular y como posibles causales, se ha considerado el estrés oxidativo y la falta de factores de crecimiento. Con el objetivo de evaluar el efecto de FGF-2 sobre la senescencia de un cultivo de células madre mesenquimales aisladas de gelatina de Wharton y su papel en la regulación del estrés oxidativo se añadieron dosis de 3,5 y 7,5 ng de FGF-2 al cultivo. Durante los pasajes 5 y 7, se estimó tanto la senescencia celular como la presencia de ROS (especies reactivas de oxígeno). Como resultado se obtuvo en el pasaje 5, una diferencia significativa del 99,5% entre el control (+) con respecto a los tratamientos con FGF-2, sin embargo, en el pasaje 7 se observó un aumento en la producción de la enzima β-galactosidasa y cambios morfológicos, confirmando un estado senescente en el cultivo en todos los tratamientos evaluados. En conclusión, las dosis utilizadas en este estudio contribuyeron positivamente a disminuir el proceso senescente en el cultivo celular, además se determinó, que el FGF-2 puede prolongar el tiempo de cultivo, retardando parcialmente la concentración de especies reactivas de oxígeno.
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NOVA por http://www.unicolmayor.edu.co/publicaciones/index.php/nova se distribuye bajo una Licencia Creative Commons Atribución-NoComercial-SinDerivar 4.0 Internacional.
Así mismo, los autores mantienen sus derechos de propiedad intelectual sobre los artículos.
Referencias
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https://doi.org/10.1155/2020/1321283
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https://doi.org/10.3390/ijms17071164
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https://doi.org/10.1038/s41583-020-0325-z
[22] K. Endo, N. Fujita, T. Nakagawa, and R. Nishimura, "Effect of fibroblast growth factor-2 and serum on canine mesenchymal stem cell chondrogenesis," Tissue Eng. Part A, vol. 25, no. 11-12, pp. 901-910, 2019.
https://doi.org/10.1089/ten.tea.2018.0177
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[27] M. Liu et al., "Adipose-derived mesenchymal stem cells from the elderly exhibit decreased migration and differentiation abilities with senescent properties," Cell Transplant., vol. 26, no. 9, pp. 1505-1519, 2017.
https://doi.org/10.1177/0963689717721221
[28] W. Zhai et al., "Identification of senescent cells in multipotent mesenchymal stromal cell cultures: Current methods and future directions," Cytotherapy, vol. 21, no. 8. 2019, doi: 10.1016/j.jcyt.2019.05.001.
https://doi.org/10.1016/j.jcyt.2019.05.001
[29] X. Meng, M. Xue, P. Xu, F. Hu, B. Sun, and Z. Xiao, "MicroRNA profiling analysis revealed different cellular senescence mechanisms in human mesenchymal stem cells derived from different origin," Genomics, vol. 109, no. 3-4, 2017, doi: 10.1016/j.ygeno.2017.02.003. https://doi.org/10.1016/j.ygeno.2017.02.003
[30] J. Franzen et al., "Senescence-associated DNA methylation is stochastically acquired in subpopulations of mesenchymal stem cells," Aging Cell, vol. 16, no. 1, 2017, doi: 10.1111/acel.12544. https://doi.org/10.1111/acel.12544
[31] J. A. Arévalo Romero, D. Páez Guerrero, and V. M. Rodríguez Pardo, "Evaluación de características morfológicas e inmunofenotipo de células madre mesenquimales en cultivo obtenidas a partir de sangre de cordón umbilical y médula ósea.," Nova, vol. 5, no. 8, 2007, doi: 10.22490/24629448.380.
https://doi.org/10.22490/24629448.380
[32] I. Fridlyanskaya, L. Alekseenko, and N. Nikolsky, "Senescence as a general cellular response to stress: a mini-review," Exp. Gerontol., vol. 72, pp. 124-128, 2015. https://doi.org/10.1016/j.exger.2015.09.021
[33] R. A. Avelar et al., "A multidimensional systems biology analysis of cellular senescence in aging and disease," Genome Biol., vol. 21, no. 1, 2020, doi: 10.1186/s13059-020-01990-9. https://doi.org/10.1186/s13059-020-01990-9
[34] D. N. Gala and Z. Fabian, "To Breathe or Not to Breathe: The Role of Oxygen in Bone Marrow-Derived Mesenchymal Stromal Cell Senescence," Stem Cells Int., vol. 2021, p. 8899756, 2021, doi: 10.1155/2021/8899756.
https://doi.org/10.1155/2021/8899756
[35] J. Li et al., "Down-regulation of fibroblast growth factor 2 (FGF2) contributes to the premature senescence of mouse embryonic fibroblast," Med. Sci. Monit., vol. 26, 2020, doi: 10.12659/MSM.920520.
https://doi.org/10.12659/MSM.920520
[36] U. Galderisi et al., "Efficient cultivation of neural stem cells with controlled delivery of FGF-2," Stem Cell Res., vol. 10, no. 1, 2013, doi: 10.1016/j.scr.2012.09.001. https://doi.org/10.1016/j.scr.2012.09.001
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https://doi.org/10.1155/2016/8479718
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