Aislamiento de bacterias potencialmente degradadoras de petróleo en hábitats de ecosistemas costeros en la Bahía de Cartagena, Colombia
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Echeverri Jaramillo, G., Manjarrez Paba, G., & Cabrera Ospino, M. (2010). Aislamiento de bacterias potencialmente degradadoras de petróleo en hábitats de ecosistemas costeros en la Bahía de Cartagena, Colombia. NOVA, 8(13). https://doi.org/10.22490/24629448.441
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Autores
Gustavo Eugenio Echeverri Jaramillo
Ganiveth Manjarrez Paba
Melody Cabrera Ospino
Resumen
En esta investigación se aislaron bacterias de 4 hábitats en el ecosistema marino aledaño a una industria petroquímica en la Bahía de Cartagena. En el proceso, las muestras se sometieron a pre-enriquecimiento por una semana, a enriquecimiento por tres semanas y a un proceso de selección de cepas competitivas, donde se evidenciaron cambios marcados en las propiedades del crudo de petróleo, como en turbidez y agregados blancos por crecimiento bacteriano. Se aislaron diferentes morfotipos que al caracterizar bioquímicamente fueron identificados como Pseudomonas aeruginosa en todas las muestras, corroborando su gran capacidad de adaptación en ambientes contaminados de este tipo. Estos resultados permitirán la realización de pruebas de biodegradación con esta bacteria y desarrollar ensayos a nivel microcosmos para su uso potencial en procesos de biorremediación de aguas marinas contaminadas con petróleo.
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NOVA por http://www.unicolmayor.edu.co/publicaciones/index.php/nova se distribuye bajo una Licencia Creative Commons Atribución-NoComercial-SinDerivar 4.0 Internacional.
Así mismo, los autores mantienen sus derechos de propiedad intelectual sobre los artículos.
Referencias
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7. Chaerun S, Tazaki K, Asada R, Kogure K. Bioremediation of coastal areas 5 years after the Nakhodka oil spill in the Sea of Japan: isolation and characterization of hydrocarbon degrading bacteria. Environmental International. 2004. 30: 911–922.
8. Chesneau H. The silent fuel killers (stability and microbiologicals). En: Proceedings of 2000 International Joint Power Generation Con ference; 2000. P. 1–8.
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marine bacteria from puget sound sediments. Applied and Environmental Microbiology. 1996; 62 (9), 3344–3349.
13. Gallego J, Loredo J, Llamas J, Vásquez F, Sánchez J. Biorremediation of diesel – contaminated soils: Evaluation of potential in situ
techniques by study of bacterial degradation. Biodegradation. 2001; 12:325-335.
14. Gaylarde C, Bento F, Kelley J. Microbial contamination of stored hydrocarbon fuels and its control. Rev Microbiol.1999; 30:1–10.
15. Gómez M, Hurtado C, Dussan J, Parra J, Narváez S. Determinación de la capacidad de degradación de compuestos orgánicos persistentes por bacterias marinas aisladas de sedimentos en el Caribe Colombiano. Actual Biol. 2006; 28 (85):125-137.
16. Harayama S, Yuki K, Akihiro H. Microbial communities in oil-contaminated seawater. Current Opinion in Biotechnology. 2004; 15:205-214.
17. Hasanuzzaman M, Umadhay-Briones K, Zsiros S, Morita N, Nodasaka Y, Yumoto I, et al. Isolation, identification, and characterization of a novel, oil-degrading bacterium, Pseudomonas aeruginosa T1. Curr Microbiol. 2004; 49:108–114.
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23. Li H, Zhao Q, Boufadel M, Venosa A. A universal nutrient application strategy for the bioremedation of oil-polluted beaches. Marine Pollution Bulletin. 2007. 54 (8): 1146-1161.
24. Mackey A, Hodgkinson M. Assesment of the impact of Naphthalene contamination on mangrove fauna using behavioral bioassays. Bulletin of Environmental Contamination and Toxicology. 1996; 56: 279–286.
25. Martínez A, Gaju N. El papel de los tapetes microbianos en la bio recuperación de zonas litorales sometidas a la contaminación por
vertidos de petróleo. Revista Ecosistemas [revista en Internet] 2005 Mayo. [acceso 19 de octubre de 2010]; 14 (2). Disponible en: http://www.revistaecosistemas.net/revista_frame.asp?pagina=%2Farticulo.asp%3Fid%3D122
26. Mashreghi M, Marialigeti K. Characterization of bacteria degrading petroleum derivates isolated from contaminated soil and water. Journal of Sciences. 2005; 16(4): 317-320.
27. Matsumiya Y, Wakita D, Kimura A, Sanpa S, Kubo M. Isolation and characterization of a lipid-degrading bacterium and its application to lipidcontaining wastewater treatment. J Biosci Bioeng. 2007; 103: 325–330.
28. Murzaev N. About microbiological methods of serum removal from oils. Mikrobiologiia. 1964; 33(6):1082–1091.
29. Narváez M, Martínez M. Selección de bacterias con capacidad degradadora de hidrocarburos, aisladas a partir de sedimentos del Caribe Colombiano. Bol Invest Mar Cost Colombia. 2008; 37 (1): 63-77.
30. Pérez R, Camacho M, Gómez J, Ábalos A, Viñas M, Cantero D. Aislamiento y selección de una cepa bacteriana degradadora de hidrocarburos a partir de suelos contaminados con petróleo. Revista CENIC Ciencias Biológicas. 2008. 39: 44-51.
31. Rolling W, Head I, Larter S. The microbiology of hydrocarbon degradation in subsurface petroleum reservoirs: perspectives and prospects. Res Microbiol. 2003; 154(5):321–328.
32. Rozanova E. Distribution of microflora in oil strata operated over long periods of time.Mikrobiologiia. 1971; 40(2):363–369.
33. Roy S, Hens D, Biswas D, Biswas D, Kumar R. Survey of petroleumdegrading bacteria in coastal waters of Sunderban Biosphere Reserve. World Journal of Microbiology & Biotechnology. 2002; 18: 575–581.
34. Ruberto L, Vazquez S, Mac Cormack W. Efectivennes of the natural becterial flora: Biostimulation and bioaugmentation on the bioremediation of a hydrocarbon contaminated Antartic soil. International Biodeterioration & Biodegradation. 2003; 52:115-125.
35. Samuel R, Stack A, y Weston L. Biosurfactant Production by Crude Oil-Degrading Microorganisms. Proc. 96th Meeting of Gen. Am. Soc. on Microbiology. 1996; p. 439.
36. Shriadah M. Impacts of an oil spill on the marine environment of the United Arab Emirates along the Gulf of Oman. Marine Pollution Bulletin. 1998. 36: 876–879.
37. Shtina, E, Nekrasova K. Vosstanovlenie neftezagryaznennykh pochvennykhekosistem (Reduction of Oil-Polluted Soil Ecosystems). Moscow: Nauka. 1998; 57–81.
38. Sikkema J, Bont J, Poolman B. Mechanisms of membrane toxicity of hydrocarbons. Microbiological Reviews. 1995; 59:201 – 222.
39. Tanaka D, Takashima M, Mizuta A, Tanaka S, Sakatoku A, Nishikawa A. Acinetobacter sp. Ud-4 Efficiently degrades both edible and mineral oils: Isolation and characterization. Curr Microbiol. 2010; 60: 203 – 209.
40. Ueno A, Hasanuzzaman H, Yumoto I, Okuyama H. Verification of Degradation of n-Alkanes in Diesel Oil by Pseudomonas aeruginosa Strain WatG in Soil Microcosms. Current microbiology. 2006; 52: 182 – 185.
41. Vargas P, Cuellar R, Dussan J. Biorremediación de residuos de petróleo. Hipótesis – apuntes científicos Uniandinos. 2004; 4:44-49.
42. Vishnyakova T, Vlasova I, Grechyushkina N, Krylov I, Paushkin Y, Litvinenko S, et al. Biological contamination of oil and oil products and their protaction during transport and storage. Review. En: Vishnyakova TP, editor. CNIITEneftehim, M, Russian; 1970.
43. Watanabe K, Kodama Y, Kaku N. Diversity and abundance of bacteria in an anderground oil-storage cavity. BMC Microbiol. 2002; 2:23.
44. Wilhelms A, Larter S, Head I, Farrimond P, di-Primio R, Zwach C. Biodegradation of oil in uplifted basins prevented by deep-burial
sterilization. Nature. 2001; 411(6841):1034–1037.
45. Yang S, Chen C, Sung Y, Lin Y. Effect of moisture content on the microbial activity in JP-5 fuel oil. Chinese J Microbiol Immunol. 1992; 25(4):223–231.
46. Yi-Chen Liu, Ling-Zhi Li, Ying Wua, Wei Tian, Li-Ping Zhang, Lian Xu, et al. Isolation of an alkane-degrading Alcanivorax sp. strain 2B5 and cloning of the alkB gene. Bioresource Technology. 2010; 101: 310–316.
47. Zahed M, Aziz H, Isa M, Mohajeri L, Mohajeri S. Optimal conditions for bioremediation of oily seawater. Bioresource Technology. (2010) 10: 9455-9460.
48. Zhengzhi Z, Zhaowei H, Chunyu Y, Cuiqing M, Fei T, Ping X. Degradation of n-alkanes and polycyclic aromatic hydrocarbons in petroleum by a newly isolated Pseudomonas aeruginosa DQ8. Bioresource Technology. 2011;102: 4111–4116.
-------------------------------------------------------------------------------
DOI: http://dx.doi.org/10.22490/24629448.441
2. Al-Mailem D, Sorkhoh N, Salamah S, Eliyas M, Radwan S. Oilbioremediation potential of Arabian Gulf mud flats rich in diazotrophic
hydrocarbon-utilizing bacteria. International Biodeterioration & Biodegradation. 2010; 64: 218-225.
3. Allsopp D, Seal K, Gaylarde C. Introduction to biodeterioration, 2a ed. Cambridge University Press, UK; 2004.
4. AL-Saleh E, Drobiova H, Obuekwe C. Predominant culturable crude oil-degrading bacteria in the coast of Kuwait. International Biodeter Biodegr . 2009; 63: 400-406.
5. Bacosa H, Suto K, Inoue C. Preferential degradation of aromatic hydrocarbons in kerosene by a microbial consortium. International Biodeterioration & Biodegradation. 2010; 64:702 - 710.
6. Bracho M, Díaz L, Soto L. M. Biodegradación de hidrocarburos aromáticos policíclicos y heterocíclicos por Pseudomonas spp. Ciencia. 2004; 12:269-275.
7. Chaerun S, Tazaki K, Asada R, Kogure K. Bioremediation of coastal areas 5 years after the Nakhodka oil spill in the Sea of Japan: isolation and characterization of hydrocarbon degrading bacteria. Environmental International. 2004. 30: 911–922.
8. Chesneau H. The silent fuel killers (stability and microbiologicals). En: Proceedings of 2000 International Joint Power Generation Con ference; 2000. P. 1–8.
9. Cho B H, Chino H, Tsuji H, Kunito T, Nagaoka K, Otsuka S, et al. Analysis of oil components and hydrocarbon-utilizing microorganisms during laboratory scale bioremediation of oil-contaminated soil of Kuwait. Chemosphere. 1997; 35 (7): 1613-1622.
10. Cohen Y. Bioremediation of oil by marine microbial mats. International Microbiology. 2002. 5:189–193.
11. Ferrari M, Neirotti E, Albornoz C. Occurrence of heterotrophic bacteria and fungi in an aviation fuel handling system and its relationship with fuel fouling. Rev Argent Microbiol.1998; 30(3):105–114.
12. Geiselbrecht A, Herwig R, Deming J, Staley J,.Enumeration and phylogenetic analysis of polycyclic aromatic hydrocarbon-degrading
marine bacteria from puget sound sediments. Applied and Environmental Microbiology. 1996; 62 (9), 3344–3349.
13. Gallego J, Loredo J, Llamas J, Vásquez F, Sánchez J. Biorremediation of diesel – contaminated soils: Evaluation of potential in situ
techniques by study of bacterial degradation. Biodegradation. 2001; 12:325-335.
14. Gaylarde C, Bento F, Kelley J. Microbial contamination of stored hydrocarbon fuels and its control. Rev Microbiol.1999; 30:1–10.
15. Gómez M, Hurtado C, Dussan J, Parra J, Narváez S. Determinación de la capacidad de degradación de compuestos orgánicos persistentes por bacterias marinas aisladas de sedimentos en el Caribe Colombiano. Actual Biol. 2006; 28 (85):125-137.
16. Harayama S, Yuki K, Akihiro H. Microbial communities in oil-contaminated seawater. Current Opinion in Biotechnology. 2004; 15:205-214.
17. Hasanuzzaman M, Umadhay-Briones K, Zsiros S, Morita N, Nodasaka Y, Yumoto I, et al. Isolation, identification, and characterization of a novel, oil-degrading bacterium, Pseudomonas aeruginosa T1. Curr Microbiol. 2004; 49:108–114.
18. Hill E. Biodeterioration of petroleum products. En: Proc. of IX Symposium Microbiology Inst. of Petrol; 1967. P.19-20.
19. Holloway B. Pseudomonas in the late twentieth century. En: Galli E, Silver S, Witholt B, edición. Pseudomonas molecular biology and biotechnology. Washington, DC: American Society for Microbiology. 1992.
20. Instituto de Investigaciones Marinas INVEMAR. Informe del estado de los ambientes marinos y costeros en Colombia. Serie de Publicaciones periódicas. 2006; 8.
21. Harayama K, Robert A, Shigeaki harayama k. Biodegradation of High- Molecular-Weight Polycyclic Aromatic Hydrocarbons by Bacteria. Journal of Bacteriology. 2000182( 8): 2059–2067
22. Kaplan C, Kitts C. Bacterial Succession in a petroleum land treatment unit. Applied and Environmental Microbiology. 2004; 70 (3): 1777 –1786.
23. Li H, Zhao Q, Boufadel M, Venosa A. A universal nutrient application strategy for the bioremedation of oil-polluted beaches. Marine Pollution Bulletin. 2007. 54 (8): 1146-1161.
24. Mackey A, Hodgkinson M. Assesment of the impact of Naphthalene contamination on mangrove fauna using behavioral bioassays. Bulletin of Environmental Contamination and Toxicology. 1996; 56: 279–286.
25. Martínez A, Gaju N. El papel de los tapetes microbianos en la bio recuperación de zonas litorales sometidas a la contaminación por
vertidos de petróleo. Revista Ecosistemas [revista en Internet] 2005 Mayo. [acceso 19 de octubre de 2010]; 14 (2). Disponible en: http://www.revistaecosistemas.net/revista_frame.asp?pagina=%2Farticulo.asp%3Fid%3D122
26. Mashreghi M, Marialigeti K. Characterization of bacteria degrading petroleum derivates isolated from contaminated soil and water. Journal of Sciences. 2005; 16(4): 317-320.
27. Matsumiya Y, Wakita D, Kimura A, Sanpa S, Kubo M. Isolation and characterization of a lipid-degrading bacterium and its application to lipidcontaining wastewater treatment. J Biosci Bioeng. 2007; 103: 325–330.
28. Murzaev N. About microbiological methods of serum removal from oils. Mikrobiologiia. 1964; 33(6):1082–1091.
29. Narváez M, Martínez M. Selección de bacterias con capacidad degradadora de hidrocarburos, aisladas a partir de sedimentos del Caribe Colombiano. Bol Invest Mar Cost Colombia. 2008; 37 (1): 63-77.
30. Pérez R, Camacho M, Gómez J, Ábalos A, Viñas M, Cantero D. Aislamiento y selección de una cepa bacteriana degradadora de hidrocarburos a partir de suelos contaminados con petróleo. Revista CENIC Ciencias Biológicas. 2008. 39: 44-51.
31. Rolling W, Head I, Larter S. The microbiology of hydrocarbon degradation in subsurface petroleum reservoirs: perspectives and prospects. Res Microbiol. 2003; 154(5):321–328.
32. Rozanova E. Distribution of microflora in oil strata operated over long periods of time.Mikrobiologiia. 1971; 40(2):363–369.
33. Roy S, Hens D, Biswas D, Biswas D, Kumar R. Survey of petroleumdegrading bacteria in coastal waters of Sunderban Biosphere Reserve. World Journal of Microbiology & Biotechnology. 2002; 18: 575–581.
34. Ruberto L, Vazquez S, Mac Cormack W. Efectivennes of the natural becterial flora: Biostimulation and bioaugmentation on the bioremediation of a hydrocarbon contaminated Antartic soil. International Biodeterioration & Biodegradation. 2003; 52:115-125.
35. Samuel R, Stack A, y Weston L. Biosurfactant Production by Crude Oil-Degrading Microorganisms. Proc. 96th Meeting of Gen. Am. Soc. on Microbiology. 1996; p. 439.
36. Shriadah M. Impacts of an oil spill on the marine environment of the United Arab Emirates along the Gulf of Oman. Marine Pollution Bulletin. 1998. 36: 876–879.
37. Shtina, E, Nekrasova K. Vosstanovlenie neftezagryaznennykh pochvennykhekosistem (Reduction of Oil-Polluted Soil Ecosystems). Moscow: Nauka. 1998; 57–81.
38. Sikkema J, Bont J, Poolman B. Mechanisms of membrane toxicity of hydrocarbons. Microbiological Reviews. 1995; 59:201 – 222.
39. Tanaka D, Takashima M, Mizuta A, Tanaka S, Sakatoku A, Nishikawa A. Acinetobacter sp. Ud-4 Efficiently degrades both edible and mineral oils: Isolation and characterization. Curr Microbiol. 2010; 60: 203 – 209.
40. Ueno A, Hasanuzzaman H, Yumoto I, Okuyama H. Verification of Degradation of n-Alkanes in Diesel Oil by Pseudomonas aeruginosa Strain WatG in Soil Microcosms. Current microbiology. 2006; 52: 182 – 185.
41. Vargas P, Cuellar R, Dussan J. Biorremediación de residuos de petróleo. Hipótesis – apuntes científicos Uniandinos. 2004; 4:44-49.
42. Vishnyakova T, Vlasova I, Grechyushkina N, Krylov I, Paushkin Y, Litvinenko S, et al. Biological contamination of oil and oil products and their protaction during transport and storage. Review. En: Vishnyakova TP, editor. CNIITEneftehim, M, Russian; 1970.
43. Watanabe K, Kodama Y, Kaku N. Diversity and abundance of bacteria in an anderground oil-storage cavity. BMC Microbiol. 2002; 2:23.
44. Wilhelms A, Larter S, Head I, Farrimond P, di-Primio R, Zwach C. Biodegradation of oil in uplifted basins prevented by deep-burial
sterilization. Nature. 2001; 411(6841):1034–1037.
45. Yang S, Chen C, Sung Y, Lin Y. Effect of moisture content on the microbial activity in JP-5 fuel oil. Chinese J Microbiol Immunol. 1992; 25(4):223–231.
46. Yi-Chen Liu, Ling-Zhi Li, Ying Wua, Wei Tian, Li-Ping Zhang, Lian Xu, et al. Isolation of an alkane-degrading Alcanivorax sp. strain 2B5 and cloning of the alkB gene. Bioresource Technology. 2010; 101: 310–316.
47. Zahed M, Aziz H, Isa M, Mohajeri L, Mohajeri S. Optimal conditions for bioremediation of oily seawater. Bioresource Technology. (2010) 10: 9455-9460.
48. Zhengzhi Z, Zhaowei H, Chunyu Y, Cuiqing M, Fei T, Ping X. Degradation of n-alkanes and polycyclic aromatic hydrocarbons in petroleum by a newly isolated Pseudomonas aeruginosa DQ8. Bioresource Technology. 2011;102: 4111–4116.
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DOI: http://dx.doi.org/10.22490/24629448.441
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